EVALUATION OF FRIESIAN HOLSTEIN GRADE COWS FERTILITY BASED THE LEVEL OF MILK UREA

D. T. Widayati, Y. Suranindyah, L. Rahmah, B. P. Widyobroto
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Abstract

This study was conducted to evaluate fertility grade of Friesian Holstein cows based on milk urea levels. A number of eight cows which fulfill the criteria of not pregnant and in second to sixth lactation, produced milk of 2.5 liters/day, and body weight ranging from 400-450 kg were used in this research. The cows were given fodder that consisted of 30 kg of forage and 10 kg of a mixture of concentrate (pollard, pulp out, and molasses), in the morning and afternoon. Milk and blood samples were taken once a week for five weeks in the morning before fed. Milk urea levels were measured using the urea FS (DiaSys) and blood samples were tested using enzyme linked immuno-sorbent assay (ELISA) with the progesterone hormone kit (DRG, Germany). The variables measured were pregnancy rate, service per conception (S/C), days open, and progesterone levels. Reproduction and milk urea levels data were analyzed by linear regression to determine the relationship between two variables. Progesterone data were analyzed descriptively. The results showed that the average calving interval, days open, and S/C were 607.5±170.27 days; 341.25±187.65 days; and 2.41±2.32 days, respectively. The average of milk urea levels was 14.16±2.55 mg/dL. Regression analysis showed a significant effect (P<0.05) and negative correlation between milk urea level and S/C. The average of non-pregnant progesterone hormone level was 12.04±7.13 ng/mL. In conclusion, the fertility grade of Friesian Holstein cows decreased when the milk urea level at 14.16 mg/dL and non-pregnant progesterone at 12.04±7.13 ng/mL.
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基于乳尿素水平评价荷斯坦奶牛育肥力
本研究以乳尿素水平为基础,评价弗里西亚荷斯坦奶牛的生育等级。本研究选用了8头符合未怀孕、泌乳2 ~ 6次标准、产奶量2.5升/天、体重400 ~ 450公斤的奶牛。在上午和下午给奶牛喂饲料,饲料由30公斤的牧草和10公斤的混合精料(pollard, pulp out和糖蜜)组成。每周1次,连续5周,在饲喂前的早晨采集牛奶和血液样本。使用尿素FS (DiaSys)检测牛奶尿素水平,使用酶联免疫吸附试验(ELISA)检测血液样本,使用孕激素试剂盒(DRG,德国)。测量的变量包括妊娠率、每次受孕服务(S/C)、开放天数和黄体酮水平。采用线性回归分析繁殖和乳尿素水平数据,确定两个变量之间的关系。对孕激素数据进行描述性分析。结果表明:平均产犊间隔、开犊天数和S/C为607.5±170.27 d;341.25±187.65天;分别为2.41±2.32天。牛奶尿素水平平均值为14.16±2.55 mg/dL。回归分析显示,乳尿素水平对S/C有显著影响(P<0.05),且呈负相关。非妊娠期黄体酮平均水平为12.04±7.13 ng/mL。综上所述,乳尿素水平为14.16 mg/dL,非孕酮水平为12.04±7.13 ng/mL时,荷斯坦奶牛的生育等级下降。
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