A SERS-based immunochromatographic assay for ultrasensitive and quantitative detection of florfenicol using long wavelength absorption of Au nanocubes

IF 1.7 3区农林科学 Q3 CHEMISTRY, APPLIED Food and Agricultural Immunology Pub Date : 2022-10-04 DOI:10.1080/09540105.2022.2120852

Ying Liu, Ting Zhou, Penghui Zhou, Hanwen Liu, Jianguo Li, Anping Deng

引用次数: 0

Abstract

ABSTRACT An immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for ultrasensitive and quantitative detection of florfenicol (FF) in food samples was developed. Au nanocubers (AuNCs) with long wavelength absorption were synthesised, characterised and used as the substrate in SERS-ICA. Immunoprobe was prepared by linking Raman reporter and the antibody against FF on AuNCs. The SERS-ICA was completed in 15 min. By measuring the Raman intensities on T lines, quantitative detection of FF was achieved. The IC50 and limit of detection (LOD) of the assay for FF were 0.027 ng mL−1 and 0.12 pg mL−1, respectively. The cross-reactivity (CR) of the assay with thiamphenicol, chloramphenicol and other four drugs were examined. The recoveries of FF in spiked samples were 88.5% ∼ 102.4% with RSD of 2.3% ∼ 7.4%. It was proven that SERS-ICA was able to rapidly detect FF in food samples with high sensitivity, accuracy and precision.

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基于SERS的金纳米立方体长波长吸收免疫色谱法超灵敏定量检测氟苯尼考

摘要建立了一种基于表面增强拉曼散射（SERS）的免疫色谱法（ICA），用于食品样品中氟苯尼考（FF）的超灵敏定量检测。合成了具有长波长吸收的Au纳米立方体（AuNCs），对其进行了表征，并将其用作SERS-ICA的基底。通过在AuNCs上连接拉曼报告子和针对FF的抗体来制备免疫探针。SERS-ICA于15年完成 min。通过测量T线上的拉曼强度，实现了FF的定量检测。FF测定的IC50和检测限（LOD）为0.027 ng mL−1和0.12 pg mL−1。检测了该方法与氯霉素、硫酚等4种药物的交叉反应性（CR）。加标样品中FF的回收率为88.5%～102.4%，RSD为2.3%～7.4%。SERS-ICA能够快速检测食品样品中的FF，具有较高的灵敏度、准确性和精密度。

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来源期刊

Food and Agricultural Immunology 农林科学-毒理学

CiteScore

5.30

自引率

6.70%

发文量

审稿时长

2 months

期刊介绍： Food and Agricultural Immunology is an international open access journal publishing original immunological research with applications in food, agricultural, environmental and veterinary science. Submissions describing the use of immunological techniques and methods are particularly welcomed. The journal aims to expand our understanding of the interactions at the interface of food and immune systems including studies on: -Development of diagnostic systems – all types of ligand-based assays, e.g. antibody, aptamer -Application of ligand-based assays for the detection or identification of molecules of interest in food science, agricultural research, veterinary investigations and clinical systems relating to food allergy or sensitivity to agricultural chemicals -Effects of food on the immune system -Studies on allergy and allergic reactions -Investigations into food allergies -Development of allergen-free food systems -Development of novel assay formats -Applications of assay systems to the monitoring of food items in relation to safety and labelling -Food quality issues, e.g. speciation, adulteration and contamination -Comparisons between different analytical techniques The journal publishes research and review articles and is essential reading for food scientists, immunologists and all those concerned with the interaction between food and immune systems.