Improving Uniformity of Cell Distribution in Post-Inkjet-Based Bioprinting

IF 2.4 3区 工程技术 Q3 ENGINEERING, MANUFACTURING Journal of Manufacturing Science and Engineering-transactions of The Asme Pub Date : 2023-08-08 DOI:10.1115/1.4063134
Jiachen Liu, Changxue Xu
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Abstract

Advancements of additive manufacturing enable fabrication of in vitro biomimetic grafts leveraging biological materials and cells for various biomedical applications. The realization of such biofabrication typically requires time from minutes to hours depending on the scale and complexity of the models. During direct biofabrication, cell sedimentation with the resultant aggregation is extensively deemed to be one of the acute problems for precise and reliable inkjet-based bioprinting. It often results in highly unstable droplet formation, nozzle clogging, and non-uniformity of post-printing cell distribution. Our previous study has implemented active bioink circulation to mitigate cell sedimentation and aggregation within the bioink reservoir. This study focuses on the comparison of post-printing cell distribution within formed microspheres and one-layer sheets with and without active circulation. The experimental results have demonstrated the significant improvement in post-printing cell distribution under implemented active circulation. Moreover, the printed sheet samples are subject to 3-day incubation to investigate the effect of cell distribution on cell viability and proliferation. It shows that compared to non-uniform cell distribution the uniform cell distribution significantly improves cell viability (92% vs 77% at Day 3) and cell proliferation (3.3 times vs 1.7 times at Day 3). The preliminary results in this letter have demonstrated not only the high effectiveness of the active bioink circulation to improve post-printing cell distribution within microspheres and one-layer sheets, but also the critical role of the uniform post-printing cell distribution in promoting cell viability and proliferation.
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提高喷墨后生物打印中细胞分布的均匀性
增材制造的进步使得能够利用生物材料和细胞制造用于各种生物医学应用的体外仿生移植物。这种生物制造的实现通常需要几分钟到几个小时的时间,这取决于模型的规模和复杂性。在直接生物制造过程中,细胞沉淀和由此产生的聚集被广泛认为是精确可靠的基于喷墨的生物打印的严重问题之一。它经常导致高度不稳定的液滴形成、喷嘴堵塞和印刷后细胞分布的不均匀。我们之前的研究已经实施了活性生物墨水循环,以减轻生物墨水库中的细胞沉积和聚集。本研究的重点是比较在有和没有活性循环的情况下,打印后细胞在形成的微球和单层片中的分布。实验结果表明,在实施主动循环的情况下,印刷后细胞分布显著改善。此外,对印刷的薄片样品进行3天的孵育,以研究细胞分布对细胞活力和增殖的影响。结果表明,与不均匀的细胞分布相比,均匀的细胞分配显著提高了细胞活力(92%对第3天的77%)和细胞增殖(3.3倍对第3天达1.7倍)。这封信中的初步结果不仅证明了活性生物墨水循环在改善微球和单层片中打印后细胞分布方面的高效性,而且还证明了打印后细胞均匀分布在促进细胞活力和增殖方面的关键作用。
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来源期刊
CiteScore
6.80
自引率
20.00%
发文量
126
审稿时长
12 months
期刊介绍: Areas of interest including, but not limited to: Additive manufacturing; Advanced materials and processing; Assembly; Biomedical manufacturing; Bulk deformation processes (e.g., extrusion, forging, wire drawing, etc.); CAD/CAM/CAE; Computer-integrated manufacturing; Control and automation; Cyber-physical systems in manufacturing; Data science-enhanced manufacturing; Design for manufacturing; Electrical and electrochemical machining; Grinding and abrasive processes; Injection molding and other polymer fabrication processes; Inspection and quality control; Laser processes; Machine tool dynamics; Machining processes; Materials handling; Metrology; Micro- and nano-machining and processing; Modeling and simulation; Nontraditional manufacturing processes; Plant engineering and maintenance; Powder processing; Precision and ultra-precision machining; Process engineering; Process planning; Production systems optimization; Rapid prototyping and solid freeform fabrication; Robotics and flexible tooling; Sensing, monitoring, and diagnostics; Sheet and tube metal forming; Sustainable manufacturing; Tribology in manufacturing; Welding and joining
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