The Anti-angiogenic Activity of Phoenix dactylifera seeds Methanol Extract in vivo study

Abstract

The Anti-angiogenic Activity of Phoenix dactylifera seeds methanol extract in vivo study Abstract: The aim of the study was to identify the antiangiogenic activity of Phoenix dactylifera seeds methanolic extract in vivo and the probable anti-angiogenic mechanism of action. The date seeds were extracted with methanol using cold method, the crude extract was tested on chick chorioallantoic membrane (CAM) as an in vivo anti-angiogenic assay. The phytochemical analysis was carried out using Fourier transform infrared spectroscopy FT-IR and Gas chromatography mass spectrometry GC-MS analysis. The results showed significant inhibition of blood vessels growth, while the phytochemical analysis showed the presence of different chemicals including phenolic compounds, terpene, fatty acids, aliphatic alcohols and others. From the data above the mechanism of action may relate to the presence of the above named compounds, as they have direct perturbing action on blood vessels and prevent cell proliferation of endothelial cells growth via their potent antioxidant and anti-inflammatory effects.