Serum of coronary atherosclerotic heart disease patients induces oxidative stress injury on endothelial cells

IF 0.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pteridines Pub Date : 2018-10-01 DOI:10.1515/pteridines-2018-0009
Huichao Pan, Min Zhang
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引用次数: 1

Abstract

Abstract Endothelial cell (EC) dysfunction has a fundamental role in the development of atherosclerosis, which leads to myocardial infarction and stroke. The aim of this study is to investigate the effect of serum from patients with coronary atherosclerotic heart disease (CAD) on endothelial cells and investigate the possible mechanism underlying these effects. Serum from 35 patients with CAD and 35 healthy volunteers was collected. Human umbilical vein endothelial cell (HUVEC) proliferation and apoptosis were assessed by a CCK‑8 assay and a flow cytometry assay, respectively. The synthesis of nitric oxide (NO) and reactive oxygen species (ROS) was measured using the nitrate reduction method and DCFH2-DA staining, respectively. The proliferation of HUVECs was inhibited by treatment with serum from CAD patients (P<0.05). Suppression of HUVEC proliferation by CAD serum occurred in a concentration-dependent manner. The synthesis of NO was also reduced in the CAD serum-treated group. Furthermore, the serum from CAD patients increased both apoptosis and intracellular ROS production in HUVECs. Moreover, treatment with tempol antagonized CAD serum-meditated HUVEC injuries. Taken together, these results suggest that HUVEC injury via CAD serum treatment is mediated by ROS production. Tempol may partly reverse this effect by abolishing HUVEC apoptosis.
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冠状动脉粥样硬化性心脏病患者血清诱导内皮细胞氧化应激损伤
内皮细胞(EC)功能障碍在动脉粥样硬化的发展中起着重要作用,动脉粥样硬化可导致心肌梗死和脑卒中。本研究的目的是探讨冠状动脉粥样硬化性心脏病(CAD)患者血清对内皮细胞的影响,并探讨这些影响的可能机制。收集了35例冠心病患者和35名健康志愿者的血清。采用CCK‑8法和流式细胞术分别评估人脐静脉内皮细胞(HUVEC)的增殖和凋亡。采用硝酸还原法和DCFH2-DA染色分别测定一氧化氮(NO)和活性氧(ROS)的合成。CAD患者血清对HUVECs增殖有抑制作用(P<0.05)。CAD血清对HUVEC增殖的抑制呈浓度依赖性。CAD血清处理组一氧化氮合成也减少。此外,CAD患者的血清增加了HUVECs的凋亡和细胞内ROS的产生。此外,用抗CAD血清治疗HUVEC损伤。综上所述,这些结果表明,通过CAD血清处理HUVEC损伤是由ROS产生介导的。Tempol可能通过消除HUVEC细胞凋亡而部分逆转这种作用。
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来源期刊
Pteridines
Pteridines 生物-生化与分子生物学
CiteScore
1.20
自引率
25.00%
发文量
6
审稿时长
>12 weeks
期刊介绍: Pteridines is an open acess international quarterly journal dealing with all aspects of pteridine research. Pteridines are heterocyclic fused ring compounds involved in a wide range of biological functions from the color on butterfly wings to cofactors in enzyme catalysis to essential vitamins. Of the pteridines, 5,6,7,8-tetrahydrobiopterin is the necessary cofactor of several aromatic amino acid monoxygenases, the nitric oxide synthases and glyceryl ether monoxygenase (GEMO). Neopterin plays an essential role in the immune system and is an important biomarker in laboratory medicine for diseases such as HIV, cardiovascular disease, malignant tumors, among others. Topics: -Neopterin, dihydroneopterin, monapterin- Biopterin, tetrahydrobiopterin- Folates, antifolates, riboflavin- Phenylalanine, tyrosine, phenylketonuria, serotonin, adrenalin, noradrenalin, L-DOPA, dopamine, related biogenic amines- Phenylalanine hydroxylase, tyrosine hydroxylase, tryptophan hydroxylase, nitric oxide synthases (iNOS), alkylglycerol monooxygenase (AGMO), dihydropterin reductase, sepiapterin reductase- Homocysteine, mediators of inflammation, redox systems, iron.
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