The parasitism of Persian jird by immature stages of Hyalomma asiaticum (Acari: Ixodidae) and its identification using molecular approaches in Iran

Abstract

In the present study, occurrence of Hyalomma asiaticum on wild rodents was explored. Rodents were trapped using Sherman traps. The tick specimens were collected by forceps from the rodents. Overall, one larva and 59 nymphs of immature ticks were collected on 23 Meriones persicus from three different locations in western Iran. A 408 bp length fragment of nuclear 5.8S /internal transcribed spacer 2 ( ITS2 ) genes was amplified in 60 examined tick specimens using PCR, of which one sample was sequenced, successfully. The BLAST results showed 99% similarity between a new haplotype from this study and two sequences of H. asiaticum from GenBank and. Therefore, we conclude that immature stages of H. asiaticum live on M. persicus and/ or their burrows. This finding helps us to better understand tick's ecology and control tick borne diseases.