Neomycin's immunomodulatory effect on the gene expression, some hematologic parameters, and intestinal histology in two rabbit lines

Q4 Biochemistry, Genetics and Molecular Biology Animal Gene Pub Date : 2022-08-01 DOI:10.1016/j.angen.2022.200130

Sarah A. Aggag, Menna-Allah E. Abdelkader, Mohamed M. Yacout

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引用次数: 2

Abstract

Understanding the effects of antibiotics on immunogen expression and the genetic consequences of antibiotic overuse in rabbit farms are becoming increasingly important as customers become more interested in healthy and safe products. The present study was destined to evaluate the effect of Neomycin, as a common antibiotic used in rabbit farms, on immune genes, some hematological parameters, and small intestinal histology in two rabbit lines: V and Alexandria. A total of 160 rabbits were orally provided 50 mg of Neomycin in 5 ml of water daily. The immunity genes have been studied using IL-2, IL-4 and CD1. The results showed an independent relationship between Neomycin-treated groups. At 21 days of age, V-line rabbits had the lowest levels of hemoglobin (Hb), red blood cells (RBCs), and lymphocytes compared to the Alexandria line. The responder rabbits showed the highest value in Hb, RBCs, and lymphocytes while the non-responder rabbits showed the highest value in WBCs. Several cytokines were tested, and no significant differences were found between the two lines. While CD1A1 (T cell surface glycoprotein CD1) increased the gene expression in the V-line innate-immune group and the responder group to the Neomycin. Based on the findings, Neomycin enhanced rabbits' immune responses.

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新霉素对两种家兔基因表达、部分血液学参数和肠道组织学的免疫调节作用

随着消费者对健康和安全的产品越来越感兴趣，了解抗生素对免疫原表达的影响以及养兔场过度使用抗生素的遗传后果变得越来越重要。本研究旨在评价新霉素作为一种常见的抗生素在家兔养殖场对两个家兔品系:V和Alexandria的免疫基因、一些血液学参数和小肠组织学的影响。160只家兔每日口服新霉素50 mg，加入5 ml水中。利用IL-2、IL-4和CD1对免疫基因进行了研究。结果显示新霉素治疗组之间存在独立关系。在21日龄时，v系家兔的血红蛋白(Hb)、红细胞(rbc)和淋巴细胞水平较亚历山大系最低。反应兔的Hb、红细胞和淋巴细胞含量最高，无反应兔的白细胞含量最高。对几种细胞因子进行了测试，发现两系之间没有明显差异。而CD1A1 (T细胞表面糖蛋白CD1)在v系先天免疫组和新霉素应答组的基因表达增加。根据研究结果，新霉素增强了兔子的免疫反应。

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Animal Gene Agricultural and Biological Sciences-Insect Science

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期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.