Oral Presentation (Best Presentation Award)

Jikken dobutsu. Experimental animals Pub Date : 2022-06-16 DOI:10.1538/expanim.71suppl-OP

Shingo Kawano, J. Bai, G. Sashida, Imari Furukawa, K. Yoshinobu, Yuri Kitamoto, Satoru Takahashi, T. Kaname, K. Araki, M. Araki

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Abstract

Sperm...have...a...characteristic...structure...called...the...“mitochondrial...sheath,”... in...which...mitochondria...are... tightly... coiled...around...the...flagellum....However,... the...molecular...mechanisms... involved... in...the... formation...of... this...structure... have...not...been...well... studied.... In... this... study,...we... investigated... the... function...of...ARMC12... that... is... specifically... expressed... in... the...testis... to...clarify... the...molecular...mechanisms... involved... in... the... formation...of... the...mitochondrial... sheath.... We...generated...several... testis-enriched...gene...KO...mice...using... the...CRISPR/Cas9...system...and...examined... their... fertility...and...sperm...morphology....Armc12...KO...male...mice...are...infertile,...and...KO...spermatozoa...showed...abnormal... morphology...and...reduced...motility....Scanning...electron...microscopy...revealed...that...sperm...mitochondria...in...KO...mice... failed... to...elongate...properly...around... the... flagellum....ARMC12... is... localized...on... the...outer...membrane...of... sperm... mitochondria...as...a...peripheral...membrane...protein.... Immunoprecipitation...revealed...that...ARMC12... interacts...with... several... proteins,... including...VDAC2... and...VDAC3,...which... are... channel... proteins... localized... on... the... outer... mitochondrial...membrane,...and...TBC1D21,...which...is...expressed...specifically...in...the...testis. Tbc1d21...KO...mice...showed...the...same...abnormalities...in...mitochondrial...sheath...formation...as...Armc12...KO...mice,...and... the...protein... interaction...between...ARMC12...and...VDAC...was...not...observed... in...Tbc1d21...KO...testis.... In...addition,... experiments...using...cultured...cells...showed...that...mitochondria... in...ARMC12-expressing...cells...aggregate,... indicating... that...ARMC12...acts...as...an...adherence...factor...between...mitochondria. These...results...suggest...that...ARMC12...acts...as...an...adherence...factor...between...mitochondria...by...cooperating...with... TBC1D21,...and...uses...VDAC...proteins...as...a...scaffold....This...suggests...that...ARMC12...enables...sperm...mitochondria...to... correctly...elongate...around...the...flagellum...during...spermatogenesis,...resulting...in...the...formation...of...a...finely...organized... mitochondrial...sheath. ARMC12 enables mitochondrial sheath formation in cooperation with TBC1D21

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口头演讲(最佳演讲奖)

精子……有……一点……。调整的特征结构…叫……“线粒体鞘,”…在线粒体…这…………紧……盘绕…在……鞭毛…然而,…分子机制……涉及到……在………形成……这个结构……不…一直…研究了…在…这个…研究中,…我们…调查………函数……ARMC12…那是……特别是……表达了……在…睪丸……为了澄清…分子机制……涉及到……在……形成……线粒体……鞘……我们生成…一些……testis-enriched基因…KO…老鼠…用…CRISPR / Cas9…系统…和…了…他们……生育…和…精子形态学……Armc12 KO……雄性老鼠…………不育,…和…KO…精子异常显示……形态学…和…减少运动性…扫描电子显微镜…透露……在…KO小鼠精子线粒体……失败了……正确地拉长……在……鞭毛ARMC12…是……本地化…在…膜外……………精子……线粒体………………外围膜蛋白质…免疫沉淀反应…了……ARMC12…交互…与…几个……蛋白质,…包括VDAC2…VDAC3…,……是……通道……蛋白质…本地化……在……外……线粒体膜,…和…TBC1D21,……这……是……表示特别………………睾丸。Tbc1d21……KO小鼠相同显示………异常…在…形成线粒体鞘………Armc12…KO…老鼠,…和…的蛋白质…互动……ARMC12…和…之间VDAC……是……不是……观察……在……Tbc1d21 KO睾丸…在…,…实验使用……培养细胞线粒体…了…………在总体…ARMC12-expressing…细胞……表明…………ARMC12……徒……是……一个……依从性的因素之间……线粒体。这些…结果…建议……ARMC12…行为…之间……一个……依从性的因素………线粒体…通过合作……TBC1D21,…和…用…VDAC…蛋白质……一个…脚手架…这表明……ARMC12…使精子线粒体……正确……拉长……在………鞭毛…在…精子形成,导致形成…………………精心组织…线粒体鞘。ARMC12与TBC1D21共同促进线粒体鞘的形成

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