Jikken dobutsu. Experimental animals最新文献

{"title":"LAS Seminar 3","authors":"","doi":"10.1538/expanim.71suppl-LAS3","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-LAS3","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S64 - S64"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47868142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symposium 6","authors":"","doi":"10.1538/expanim.71suppl-S6","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-S6","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S31 - S36"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45076147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Presentation (Best Presentation Award)","authors":"Shingo Kawano, J. Bai, G. Sashida, Imari Furukawa, K. Yoshinobu, Yuri Kitamoto, Satoru Takahashi, T. Kaname, K. Araki, M. Araki","doi":"10.1538/expanim.71suppl-OP","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-OP","url":null,"abstract":"Sperm...have...a...characteristic...structure...called...the...“mitochondrial...sheath,”... in...which...mitochondria...are... tightly... coiled...around...the...flagellum....However,... the...molecular...mechanisms... involved... in...the... formation...of... this...structure... have...not...been...well... studied.... In... this... study,...we... investigated... the... function...of...ARMC12... that... is... specifically... expressed... in... the...testis... to...clarify... the...molecular...mechanisms... involved... in... the... formation...of... the...mitochondrial... sheath.... We...generated...several... testis-enriched...gene...KO...mice...using... the...CRISPR/Cas9...system...and...examined... their... fertility...and...sperm...morphology....Armc12...KO...male...mice...are...infertile,...and...KO...spermatozoa...showed...abnormal... morphology...and...reduced...motility....Scanning...electron...microscopy...revealed...that...sperm...mitochondria...in...KO...mice... failed... to...elongate...properly...around... the... flagellum....ARMC12... is... localized...on... the...outer...membrane...of... sperm... mitochondria...as...a...peripheral...membrane...protein.... Immunoprecipitation...revealed...that...ARMC12... interacts...with... several... proteins,... including...VDAC2... and...VDAC3,...which... are... channel... proteins... localized... on... the... outer... mitochondrial...membrane,...and...TBC1D21,...which...is...expressed...specifically...in...the...testis. Tbc1d21...KO...mice...showed...the...same...abnormalities...in...mitochondrial...sheath...formation...as...Armc12...KO...mice,...and... the...protein... interaction...between...ARMC12...and...VDAC...was...not...observed... in...Tbc1d21...KO...testis.... In...addition,... experiments...using...cultured...cells...showed...that...mitochondria... in...ARMC12-expressing...cells...aggregate,... indicating... that...ARMC12...acts...as...an...adherence...factor...between...mitochondria. These...results...suggest...that...ARMC12...acts...as...an...adherence...factor...between...mitochondria...by...cooperating...with... TBC1D21,...and...uses...VDAC...proteins...as...a...scaffold....This...suggests...that...ARMC12...enables...sperm...mitochondria...to... correctly...elongate...around...the...flagellum...during...spermatogenesis,...resulting...in...the...formation...of...a...finely...organized... mitochondrial...sheath. ARMC12 enables mitochondrial sheath formation in cooperation with TBC1D21","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S65 - S76"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43386416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Encouragement Award","authors":"","doi":"10.1538/expanim.71suppl-L2","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-L2","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S2 - S3"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42950827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ando-Tajima Award","authors":"","doi":"10.1538/expanim.71suppl-L1","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-L1","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S1 - S1"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47540386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symposium 4","authors":"","doi":"10.1538/expanim.71suppl-S4","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-S4","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S22 - S24"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42003235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LAS Seminar 2","authors":"K. Mominoki","doi":"10.1538/expanim.71suppl-LAS2","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-LAS2","url":null,"abstract":"The...Japanese...Association...of...Laboratory...Animal...Facilities...of...National...University...Corporations...(JALAN)...aims... to... improve... the...accuracy...and...standard...of...animal...experiments...at...national...university...corporations,... and... to... promote...the...refinement...of...care...and...use...for...laboratory...animals....JALAN...has...been...organized...about...50...years...ago... for...the...purpose...of...establishing...a...communication...system...and...cooperation...system...between...the...animal...facilities... in...order...to...contribute...to...the...promotion...of...education...and...research...in...the...life...science....Since...its...establishment,... the...JALAN...has...been...working...with...the...recognition...that...“people”...are...important...to...support...the...animal...facilities... and... the...scientific... fields... that... form...the...basis...of... their...activities.... In...particular,... in...1971-1995,...when... the... initial... membership... facilities...were...established... in... the...national...universities,... there...were...no... institutions... for... training... “people”...such...as...experimental...animal...technologists,...so...it...is...important...to...train...those....Therefore,...we...have...been... holding...workshops...for...experimental...animal...technologists...every...year...since...1995. By...the...way,... the...workshop... in...2020...was...planned...to...be...held... jointly...by...the...two...animal... facilities... in...Hokkaido... University.... In...January,...2020,... the...first...person... infected...with...COVID-19...was...confirmed... in...Japan,...and... in...April,... 2020,... an... “emergency...declaration”...based...on... the...Act... for...Pandemic... Influenza...and...New...Infection...Diseases... Preparedness...and...Response...was...nationally...issued....Although...the...declarations...of...emergency...were...lifted...by...May... 25,...2020,... the...workshop... in...2020...was...canceled...due...to... the...strong...demand... for... thorough...measures...to...prevent... infection....The... secretariat... of... JALAN...had...decided... to...produce... the... training...video... for... animal... experiment... technologists...instead...of...the...workshop...in...2020....The...produced...video...was...distributed...to...the...JALAN...members...at... the...JALAN...on-lined...meeting...in...2021....At...this...LAS...seminar,...I...had...the...opportunity...to...introduce...this...vide,...so...I... will...explain...the...background...and... intention...of... the...video...production,...and...the...way...of...thinking...when...using...this... video. Production of Education and Training Video -Basic Techniques for Rabbits, Dogs, and Pigs Uses-","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S56 - S63"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42584455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symposium 3","authors":"","doi":"10.1538/expanim.71suppl-S3","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-S3","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S19 - S21"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42612480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Education Seminar","authors":"","doi":"10.1538/expanim.71suppl-ES","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-ES","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S47 - S50"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49091998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Award","authors":"","doi":"10.1538/expanim.71suppl-IA","DOIUrl":"https://doi.org/10.1538/expanim.71suppl-IA","url":null,"abstract":"","PeriodicalId":75961,"journal":{"name":"Jikken dobutsu. Experimental animals","volume":"71 1","pages":"S4 - S7"},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43867684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}