Effect of acupuncture at Renying (ST 9) on gene expression profile of hypothalamus in spontaneously hypertensive rats

IF 2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional Chinese Medicine Pub Date : 2018-04-01 DOI:10.1016/j.jtcm.2018.04.008

Guo Yan , Lu Juan , Liang Jingrong , Zhao Ruili , Xu Jing , Zhang Wei , Park Kibeum , Zhu Shipeng , Chen Huan , Ma Liangxiao

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引用次数: 3

Abstract

OBJECTIVE

To investigate changes in gene expression profiles in the hypothalamus related to the effects of acupuncture at the Renying (ST 9) acupoint in spontaneously hypertensive (SH) rats.

METHODS

We randomly divided 18 SH rats into Renying (ST 9) group and model control group, 9 body weight-matched Wistar-Kyoto rats were used as blank controls. Acupuncture was performed manually for 20-min daily over 28 d in the Renying (ST 9) group. Rat Gene 2.0 array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time quantitative polymerase chain reaction (RT-PCR) analyses.

RESULTS

The different groups exhibited differential gene expression: compared with the blank control group, 48 genes were up-regulated and 91 genes were down-regulated in the model group; compared with the model group, 79 genes were up-regulated and 80 genes were down-regulated in Renying (ST 9) group. The RT-PCR results of the key genes including Chi3l1, Ephx2, Klk1, 5-HT1A and Cbs were consistent with that of gene chip analysis.

CONCLUTION

Acupuncture at Renying (ST 9) could significantly lower the blood pressure of SH rats and affect their hypothalamic gene expression profile. Genes associated with the contraction of vascular smooth muscle and the regulation of inflammation, neurotransmitters may be involved in acupuncture's antihypertensive mechanism.

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针刺仁阴穴对自发性高血压大鼠下丘脑基因表达谱的影响

目的探讨针刺仁应穴对自发性高血压(SH)大鼠下丘脑基因表达谱的影响。方法将18只SH大鼠随机分为仁应组和模型对照组，9只体重匹配的Wistar-Kyoto大鼠作为空白对照。仁英(ST 9)组每日手工针刺20分钟，持续28 d。采用大鼠基因2.0阵列技术检测基因表达谱，通过实时定量聚合酶链反应(RT-PCR)对筛选出的关键基因进行验证。结果各组间基因表达存在差异:与空白对照组相比，模型组有48个基因上调，91个基因下调;与模型组比较，仁颖(ST 9)组有79个基因上调，80个基因下调。关键基因Chi3l1、Ephx2、Klk1、5-HT1A和Cbs的RT-PCR结果与基因芯片分析结果一致。结论针刺人营(ST 9)可显著降低SH大鼠血压，影响下丘脑基因表达谱。与血管平滑肌收缩和炎症调节相关的基因、神经递质可能参与了针灸的降压机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

2.40

自引率

3.80%

发文量

32269

审稿时长

2 months

期刊介绍： Journal of Traditional Chinese Medicine（JTCM） is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.