Protective Effects of Oleoylethanolamine on Diabetic Nephropathy: Role of AMP-Activated Protein Kinase/Nuclear Factor-E2-Related Factor 2 Pathway

IF 0.9 4区材料科学 Science of Advanced Materials Pub Date : 2023-04-01 DOI:10.1166/sam.2023.4460

Yong Wu, Si-ruo Li, Y. Liu, Liwen Yang, Weiping Lin

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Abstract

This study aimed to investigate the protective effect of Oleoylethanolamine (OEA) on kidney injury in diabetic nephropathy (DN) mice. Sixty C57BL/6 mice were used, and 45 mice were induced with diabetes. Thirty diabetic mice were divided into OEA low-dose and high-dose treatment groups, receiving 5 or 20 mg/kg OEA by gavage daily for 4 weeks. OEA treatment significantly improved general conditions, increased body weight, and reduced 24 h urine protein, urinary albumin, serum creatinine, and blood urea nitrogen levels. Inflammatory factors and renal inflammation were dramatically reduced after OEA intervention. OEA also increased antioxidant enzyme activity and reduced the increase of reactive oxygen species and malondialdehyde content in kidney tissues. Western blot detection revealed that OEA intervention upregulated the expression of p-AMPK and n-Nrf2 proteins in the AMPK/Nrf2 signaling pathway in renal tissue, effectively preventing the progression of DN. The study suggests that OEA’s protective effect on kidney injury in DN mice is related to the regulation of the AMPK/Nrf2 signaling pathway.

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油基乙醇胺对糖尿病肾病的保护作用:amp活化的蛋白激酶/核因子e2相关因子2通路的作用

本研究旨在探讨油酰乙醇胺（OEA）对糖尿病肾病（DN）小鼠肾损伤的保护作用。使用60只C57BL/6小鼠，并用糖尿病诱导45只小鼠。将30只糖尿病小鼠分为OEA低剂量和高剂量治疗组，每天灌胃给予5或20mg/kg OEA，持续4周。OEA治疗显著改善了一般情况，增加了体重，并降低了24小时尿蛋白、尿白蛋白、血清肌酐和血尿素氮水平。OEA干预后，炎症因子和肾脏炎症显著减少。OEA还增加了抗氧化酶活性，减少了肾组织中活性氧和丙二醛含量的增加。Western印迹检测显示，OEA干预上调了肾组织中AMPK/Nrf2信号通路中p-AMPK和n-Nrf2蛋白的表达，有效预防了DN的进展。研究表明，OEA对DN小鼠肾损伤的保护作用与AMPK/Nrf2信号通路的调节有关。

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