{"title":"Spatially resolved mapping of hydrogel stiffness during enzymatic degradation","authors":"Nathan Strong, A. Pungor, V. Hlady","doi":"10.1680/jsuin.22.01029","DOIUrl":null,"url":null,"abstract":"Atomic force microscopy force-volume technique was used to investigate the surface properties of two hydrogels during their enzymatic degradation. Agarose gels with concentrations of 0.5%, 1.0%, and 1.5% w/v were selected for the degradation study to show that the mechanical effects of degradation could be measured in a spatially resolved way on hydrogel surface. Agarase enzyme was used to degrade the agarose gels. The agarose gels were found to be heterogeneous with multiple stiffness domains that were degraded with different erosion rates. It was inferred that agarose gels contained both tightly and loosely packed regions. The loosely packed regions degraded first, followed by the regions that were packed more tightly. Second hydrogel containing hyaluronic acid and gelatin with 5% w/v hyaluronic acid and 5% w/v gelatin cross-linked in equal amounts were also studied. These hybrid gels were degraded either by using hyaluronidase or collagenase type IV. With hyaluronidase, gelatin was left on the surface as a homogenous layer. With collagenase, cross-linked hyaluronic acid remained on the surface. Post-degradation the hyaluronic acid rich surface had a stiffness of ∼20 kPa, while the gelatin rich surface stiffness was only ∼15 kPa.","PeriodicalId":22032,"journal":{"name":"Surface Innovations","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Surface Innovations","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1680/jsuin.22.01029","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 1
Abstract
Atomic force microscopy force-volume technique was used to investigate the surface properties of two hydrogels during their enzymatic degradation. Agarose gels with concentrations of 0.5%, 1.0%, and 1.5% w/v were selected for the degradation study to show that the mechanical effects of degradation could be measured in a spatially resolved way on hydrogel surface. Agarase enzyme was used to degrade the agarose gels. The agarose gels were found to be heterogeneous with multiple stiffness domains that were degraded with different erosion rates. It was inferred that agarose gels contained both tightly and loosely packed regions. The loosely packed regions degraded first, followed by the regions that were packed more tightly. Second hydrogel containing hyaluronic acid and gelatin with 5% w/v hyaluronic acid and 5% w/v gelatin cross-linked in equal amounts were also studied. These hybrid gels were degraded either by using hyaluronidase or collagenase type IV. With hyaluronidase, gelatin was left on the surface as a homogenous layer. With collagenase, cross-linked hyaluronic acid remained on the surface. Post-degradation the hyaluronic acid rich surface had a stiffness of ∼20 kPa, while the gelatin rich surface stiffness was only ∼15 kPa.
Surface InnovationsCHEMISTRY, PHYSICALMATERIALS SCIENCE, COAT-MATERIALS SCIENCE, COATINGS & FILMS
CiteScore
5.80
自引率
22.90%
发文量
66
期刊介绍:
The material innovations on surfaces, combined with understanding and manipulation of physics and chemistry of functional surfaces and coatings, have exploded in the past decade at an incredibly rapid pace.
Superhydrophobicity, superhydrophlicity, self-cleaning, self-healing, anti-fouling, anti-bacterial, etc., have become important fundamental topics of surface science research community driven by curiosity of physics, chemistry, and biology of interaction phenomenon at surfaces and their enormous potential in practical applications. Materials having controlled-functionality surfaces and coatings are important to the manufacturing of new products for environmental control, liquid manipulation, nanotechnological advances, biomedical engineering, pharmacy, biotechnology, and many others, and are part of the most promising technological innovations of the twenty-first century.