Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B1 and amantadine

IF 1.7 3区农林科学 Q3 CHEMISTRY, APPLIED Food and Agricultural Immunology Pub Date : 2022-06-14 DOI:10.1080/09540105.2022.2080188

Kezhuo Zhang, Huadong Cai, Min-jen Lu, Daixian Wei, Jiaqi Yin, Nengshui Ding, W. Lai, Juan Peng

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引用次数: 4

Abstract

ABSTRACT A bispecific monoclonal antibody (BsMAb) that can simultaneously recognize aflatoxin B1 (AFB1) and amantadine (AMD) was prepared. Quantum dot nanobead immunochromatographic assay (QB-ICA) based on the BsMAb was developed for the simultaneous detection of AFB1 and AMD in four feed samples (suckling pig feed, piglet feed, sow feed, and compound feed for laying ducks). Under optimal experimental conditions, the LOD values of QB-ICA for AFB1 in four feed samples were 0.188, 0.221, 0.233, and 0.306 μg/kg, respectively. The LOD values of QB-ICA for AMD in the same feed samples were 0.125, 0.187, 0.076, and 0.191 μg/kg, respectively. The recovery rates of QB-ICA for AFB1 and AMD ranged from 91.55% to 150.87% and from 91.62% to 138.42%, respectively. The variation coefficients were all less than 15%. The proposed QB-ICA had high reliability and specificity for the simultaneous detection of AFB1 and AMD, providing a platform for simultaneous detection of hazardous substances.

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基于双特异性单克隆抗体的量子点纳米珠免疫色谱法同时检测黄曲霉毒素B1和金刚烷胺

摘要制备了一种能同时识别黄曲霉毒素B1（AFB1）和金刚烷胺（AMD）的双特异性单克隆抗体（BsMAb）。基于BsMAb的量子点纳米珠免疫色谱法（QB-ICA）用于同时检测四种饲料样品（乳猪饲料、仔猪饲料、母猪饲料和蛋鸡复合饲料）中的AFB1和AMD。在最佳实验条件下，四个饲料样品中AFB1的QB-ICA检测限分别为0.188、0.221、0.233和0.306μg/kg。QB-ICA对相同饲料样品中AMD的LOD值分别为0.125、0.187、0.076和0.191μg/kg。QB-ICA对AFB1和AMD的回收率分别为91.55%至150.87%和91.62%至138.42%。变异系数均小于15%。所提出的QB-ICA对同时检测AFB1和AMD具有较高的可靠性和特异性，为同时检测危险物质提供了一个平台。

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来源期刊

Food and Agricultural Immunology 农林科学-毒理学

CiteScore

5.30

自引率

6.70%

发文量

审稿时长

2 months

期刊介绍： Food and Agricultural Immunology is an international open access journal publishing original immunological research with applications in food, agricultural, environmental and veterinary science. Submissions describing the use of immunological techniques and methods are particularly welcomed. The journal aims to expand our understanding of the interactions at the interface of food and immune systems including studies on: -Development of diagnostic systems – all types of ligand-based assays, e.g. antibody, aptamer -Application of ligand-based assays for the detection or identification of molecules of interest in food science, agricultural research, veterinary investigations and clinical systems relating to food allergy or sensitivity to agricultural chemicals -Effects of food on the immune system -Studies on allergy and allergic reactions -Investigations into food allergies -Development of allergen-free food systems -Development of novel assay formats -Applications of assay systems to the monitoring of food items in relation to safety and labelling -Food quality issues, e.g. speciation, adulteration and contamination -Comparisons between different analytical techniques The journal publishes research and review articles and is essential reading for food scientists, immunologists and all those concerned with the interaction between food and immune systems.