Ploidy estimation and pre-selection of banana autotetraploids after in vitro polyploidy induction

IF 0.9 4区农林科学 Q3 AGRONOMY Revista Caatinga Pub Date : 2023-09-01 DOI:10.1590/1983-21252023v36n302rc

Viviane P. Borges, D. G. Silveira, M. P. S. D. Costa, J. A. Santos-Serejo, S. O. Silva

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引用次数: 1

Abstract

ABSTRACT This study aimed to evaluate the efficiency of the parameters fresh leaf mass and stomatal density in the estimation of ploidy and pre-selection of putative banana autotetraploids. Young plants of the diploid cultivar Ouro (AA), previously subjected to in vitro polyploidization with the antimitotic amiprofos-methyl - APM (0, 10, 20, 30, 40, and 60 µM L-1) and colchicine (2.5 mM L-1) were evaluated for survival, height, number of leaves, pseudostem diameter, leaf disc fresh mass, and stomatal density. Ploidy was determined by flow cytometry in a random sample of 200 plants to find the relationship between the genomic content and the analyzed variables. Spearman’s correlation analysis showed a strong correlation (0.84**) between leaf disc fresh mass and tetraploid plants, as well as an absence of significant correlation between stomatal density and ploidy levels. Thus, the pre-selection of plants was carried out using a reference value of leaf disc fresh mass and resulted in 688 plants pre-selected as putative tetraploids. Among them, 318 were confirmed as tetraploids, 291 as diploids, and 79 as mixoploids after flow cytometry analysis. The pre-selection efficiency was 46.2% of tetraploid plants from the total pre-selected. The results indicate that the use of leaf disc fresh mass is a simple, practical, and promising method to estimate ploidy when a high number of plants is obtained in in vitro banana polyploidization studies, reducing the total number of plants to have confirmed ploidy.

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香蕉离体多倍体诱导后同源四倍体的倍性估计和预选择

摘要本研究旨在评价鲜叶质量和气孔密度参数在香蕉同源四倍体倍性估计和预选择中的有效性。以二倍体栽培品种欧罗(AA)为材料，分别用抗有丝分裂的氨异丙酯-甲基APM(0、10、20、30、40和60µM L-1)和秋水仙碱(2.5 mM L-1)进行离体多倍体分化，评价其成活率、高度、叶片数、假茎直径、叶盘新鲜质量和气孔密度。用流式细胞术对200株植物随机取样进行倍性测定，找出基因组含量与分析变量之间的关系。Spearman相关分析表明，叶片鲜质量与四倍体植株有很强的相关性(0.84**)，气孔密度与倍性水平无显著相关。因此，以叶片新鲜质量为参考值进行植物预选，共选育出688株四倍体植物。其中，经流式细胞术鉴定为四倍体318株，二倍体291株，混倍体79株。四倍体的预选效率为46.2%。结果表明，在香蕉离体多倍体研究中，当获得大量植株时，利用叶片新鲜质量来估计倍性是一种简单、实用、有前景的方法，减少了确定倍性的植株总数。

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来源期刊

Revista Caatinga AGRONOMY-

CiteScore

2.10

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： A Revista Caatinga é uma publicação científica que apresenta periodicidade trimestral, publicada pela Pró-Reitoria de Pesquisa e Pós-Graduação da Universidade Federal Rural do Semi-Árido – UFERSA, desde 1976. Objetiva proporcionar à comunidade científica, publicações de alto nível nas áreas de Ciências Agrárias e Recursos Naturais, disponibilizando, integral e gratuitamente, resultados relevantes das pesquisas publicadas.