Zinc oxide nanopriming elevates wheat drought tolerance by inducing stress-responsive genes and physio-biochemical changes

IF 5.4 Q1 PLANT SCIENCES Current Plant Biology Pub Date : 2023-09-01 DOI:10.1016/j.cpb.2023.100292
Priyanka Pandya, Sushil Kumar, Amar A. Sakure, Rutul Rafaliya, Ghanshyam B. Patil
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Abstract

Zinc oxide nanoparticles (ZnO NPs) are currently being used in a number of applications, including agriculture. In agricultural regions all throughout the world, drought poses a serious danger to crop production and development. The outcome of this experiment showed that the treatment of 250 ppm ZnO NPs provides drought resistance by considerably improving physiological and biochemical traits, viz., shoot and root length, RWC, MSI, Zn content, total chlorophyll and protein content, biomass accumulation, osmolytes content, and antioxidant enzyme activities. Similar results were found by gene expression analysis. The expression of drought-responsive genes (DHN, DREB, P5CS, BADH, SOD, CAT, APX, bZIP and NAC) were highly upregulated in ZnO- treated plants compared with non-ZnO treated root and leaf tissues of plants under stress and non-stress conditions. The osmoregulation-related genes (P5CS and BADH) were highly expressed in ZnO treated plants over non-ZnO treated samples in both conditions (stress and control). However, the relative accumulation of these genes was higher root tissues compared to leaf tissues. According to the results, ZnO NPs caused an instantaneous rise in P5CS and BADH expression, which function as stress signaling molecules and trigger the production of genes that are responsive to drought. This results in the activation of the defense system and a greater ability to withstand stress. ZnO NPs in general may, under drought conditions, influence the expression of genes that are drought-inducible via both ABA-dependent and ABA-independent pathways.

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氧化锌纳米膜通过诱导胁迫响应基因和生理生化变化提高小麦抗旱性
氧化锌纳米颗粒(ZnO NPs)目前被用于包括农业在内的许多应用领域。在世界各地的农业地区,干旱对作物生产和发展构成严重威胁。本试验结果表明,250 ppm氧化锌NPs处理通过显著改善植株的生理生化性状,即茎和根长、RWC、MSI、Zn含量、总叶绿素和总蛋白质含量、生物量积累、渗透物含量和抗氧化酶活性,提高了植株的抗旱性。基因表达分析也发现了类似的结果。胁迫和非胁迫条件下,氧化锌处理的植株根系和叶片组织中干旱响应基因(DHN、DREB、P5CS、BADH、SOD、CAT、APX、bZIP和NAC)的表达均显著上调。在胁迫和对照条件下,氧化锌处理植株的渗透调节相关基因(P5CS和BADH)均比未处理的植株高表达。但根组织中这些基因的相对积累量高于叶组织。结果表明,ZnO NPs导致P5CS和BADH的表达瞬间升高,而P5CS和BADH是胁迫信号分子,可以触发干旱响应基因的产生。这导致防御系统的激活和更大的承受压力的能力。在干旱条件下,氧化锌NPs通常可以通过aba依赖和aba不依赖的途径影响干旱诱导基因的表达。
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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