{"title":"Detection of Bovine Herpesvirus 1 from Semen by Real-time PCR to Prevent the Spread of Infectious Bovine Rhinotracheitis Infection","authors":"T. Untari, Y. P. Kristianingrum, A. Kusumawati","doi":"10.54203/scil.2021.wvj89","DOIUrl":null,"url":null,"abstract":"Infectious Bovine Rhinotracheitis (IBR) can be transmitted by livestock seeds and semen, through the process of artificial insemination. Therefore, it is necessary to detect the presence of the Bovine Herpesvirus 1 (BHV1) in semen through artificial insemination and breeding centers of Indonesia. The current study aimed to detect the presence of the virus in semen as a source of IBR disease transmission in Indonesia. A total of 27 semen samples from artificial insemination and breeding centers (Sembawa, Lembang, Ungaran, and Sleman) in Indonesia have been examined and identified using the real-time PCR (qPCR) technique. The result showed that all samples were negative to BHV1. This indicated that semen from Ungaran, Sembawa, Sleman, and Lembang was safe to be used as a semen source for artificial insemination.","PeriodicalId":52153,"journal":{"name":"World''s Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"World''s Veterinary Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.54203/scil.2021.wvj89","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Veterinary","Score":null,"Total":0}
引用次数: 0
Abstract
Infectious Bovine Rhinotracheitis (IBR) can be transmitted by livestock seeds and semen, through the process of artificial insemination. Therefore, it is necessary to detect the presence of the Bovine Herpesvirus 1 (BHV1) in semen through artificial insemination and breeding centers of Indonesia. The current study aimed to detect the presence of the virus in semen as a source of IBR disease transmission in Indonesia. A total of 27 semen samples from artificial insemination and breeding centers (Sembawa, Lembang, Ungaran, and Sleman) in Indonesia have been examined and identified using the real-time PCR (qPCR) technique. The result showed that all samples were negative to BHV1. This indicated that semen from Ungaran, Sembawa, Sleman, and Lembang was safe to be used as a semen source for artificial insemination.
期刊介绍：
The World''s Veterinary Journal (ISSN 2322-4568) is an international, peer reviewed open access journal aims to publish the high quality material from veterinary scientists'' studies. All accepted articles are published Quarterly in full text on the Internet. WVJ publishes the results of original scientific researches, reviews, case reports and short communications, in all fields of veterinary science. In details, topics are: Behavior Environment and welfare Animal reproduction and production Parasitology Endocrinology Microbiology Immunology Pathology Pharmacology Epidemiology Molecular biology Immunogenetics Surgery Virology Physiology Vaccination Gynecology Exotic animals Animal diseases Radiology Ophthalmology Dermatology Chronic disease Anatomy Non-surgical pathology issues of small to large animals Cardiology and oncology.
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