Effect of optimal combination of Huangqi (Radix Astragali Mongolici) and Ezhu (Rhizoma Curcumae Phaeocaulis) on proliferation and apoptosis of A549 lung cancer cells

IF 2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional Chinese Medicine Pub Date : 2018-06-01 DOI:10.1016/S0254-6272(18)30625-3
Xu Chengyong , Wang Yuguo , Feng Jian , Qin Li , Xu Ran , Dou Yongqi
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引用次数: 7

Abstract

OBJECTIVE

To investigate the effect of optimal combination (E) of Huangqi (Radix Astragali Mongolici) and Ezhu (Rhizoma Curcumae Phaeocaulis) on proliferation and apoptosis of A549 lung cancer cells and the possible mechanism underpinning the action.

METHODS

A uniform design method was used to optimize the E of Huangqi (Radix Astragali Mongolici) and Ezhu (Rhizoma Curcumae Phaeocaulis) in A549 lung cancer cells. MTS assay was applied to analyze the effect of the component formula of Huangqi (Radix Astragali Mongolici) and Ezhu (Rhizoma Curcumae Phaeocaulis) on A549 cells viability in various uniform design groups. A549 cells with exponential growth in routine culture were exposed to CoCl2 (200 μmol/L) to mimic hypoxic conditions. Group 0 was treated with RPMI-1640, the group CoCl2 was treated with CoCl2 (200 μmol/L), the group DDP + CoCl2 was treated with 4 mg/L Cisplatin injection (DDP) + CoCl2 (200 μmol/L), and the drug group was treated with various dose of E (0.5E, 1E, 2E) + CoCl2 (200 μmol/L). All groups were cultured for 24 h. Cell apoptosis was measured by Annexin V-FITC/propidium iodide double staining and flow cytometry. Western blot assay and quantitative real-time polymerase chain reaction (qRT-PCR) were employed to detect the protein and mRNA expression of B-celllymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax) and cysteinyl aspartate specific proteinase-3 (caspase-3).

RESULTS

The E obtained by the uniform design was comprise of 200 mg/L Astragalus polysaccharide (X1) and 32 mg/L Curcumin (X3). Group DDP+ CoCl2, group 1E + CoCl2 and group 2E + CoCl2 promoted the apoptosis of A549 cells (P < 0.05). Group 1E + CoCl2 and group 2E + CoCl2 had no statistically significant differences compared with the group DDP + CoCl2 (P > 0.05). Compared with group 0, various doses of E + CoCl2 could up-regulate the expression of Bax and caspase-3 and down-regulate the expression of Bcl-2 at protein and mRNA levels (P < 0.05).

CONCLUSION

Astragalus polysaccharide and Curcumin was the optimal combination of Huangqi (Radix Astragali Mongolici) and Ezhu (Rhizoma Curcumae Phaeocaulis). E promoted the apoptosis of A549 cells. Combination of Astragalus polysaccharide and Curcumin increased the expression of Bax and caspase-3, and decreased the expression of Bcl-2 to initiate apoptosis in A549 cells under chemical-induced hypoxia.

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黄芪与莪术最佳配伍对A549肺癌细胞增殖和凋亡的影响
目的探讨黄芪与莪术最佳配伍(E)对肺癌A549细胞增殖和凋亡的影响及其可能机制。方法采用均匀设计法优化黄芪和莪术在A549肺癌细胞中的作用。采用MTS法分析黄芪和莪术组方对不同均匀设计组A549细胞活力的影响。在常规培养中呈指数增长的A549细胞暴露于200 μmol/L的CoCl2中模拟缺氧条件。0组采用rpm -1640治疗,CoCl2组采用CoCl2 (200 μmol/L)治疗,DDP + CoCl2组采用4 mg/L顺铂注射液(DDP) + CoCl2 (200 μmol/L)治疗,药物组采用不同剂量的E (0.5E、1E、2E) + CoCl2 (200 μmol/L)治疗。各组培养24 h,采用Annexin V-FITC/碘化丙啶双染色及流式细胞术检测细胞凋亡情况。采用Western blot法和定量实时聚合酶链反应(qRT-PCR)检测b细胞淋巴瘤-2 (Bcl-2)、Bcl-2相关X蛋白(Bax)和天冬氨酸半胱氨酸特异性蛋白酶-3 (caspase-3)蛋白和mRNA的表达。结果均匀设计得到的E由200 mg/L黄芪多糖(X1)和32 mg/L姜黄素(X3)组成。DDP+ CoCl2组、1E + CoCl2组和2E + CoCl2组均促进A549细胞凋亡(P <0.05)。1E + CoCl2组、2E + CoCl2组与DDP + CoCl2组比较,差异无统计学意义(P >0.05)。与0组比较,不同剂量E + CoCl2可在蛋白和mRNA水平上调Bax和caspase-3的表达,下调Bcl-2的表达(P <0.05)。结论黄芪多糖和姜黄素是黄芪和莪术的最佳组合。E促进A549细胞凋亡。黄芪多糖与姜黄素联用可提高化学缺氧诱导A549细胞Bax和caspase-3的表达,降低Bcl-2的表达,启动细胞凋亡。
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来源期刊
Journal of Traditional Chinese Medicine
Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
2.40
自引率
3.80%
发文量
32269
审稿时长
2 months
期刊介绍: Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.
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