Development and manufacture of erythrocyte pullorosis diagnosticum for the diagnosis of avian pullorosis typhoid

Abstract

The article is devoted to the problems of diagnostics of poultry typhoid fever and the development of a technology for the manufacture of diagnosticum against this disease. A method of making erythrocyte diagnosticum for the diagnosis of poultry typhoid fever is shown, including obtaining the bacterial mass of Salmonella pullorum-gallinarum, isolating the antigenic fraction from it by treating the bacterial mass with a surfactant with the addition of soda or alkali in distilled water at 93-96 °C, followed by sensitization of formalinized erythrocytes, their purification and obtaining the target product in the form of 10% suspension, characterized in that 1-1.5% aqueous solution of Desmol is used as a surfactant to isolate the antigenic fraction, taken in a final weight concentration of 0, 1-0.3%, and the sensitization of formalinized erythrocytes is carried out in the presence of the sodium salt of chitosan succinate taken in a final weight concentration of 0.5-1.5%. In the industrial production of the diagnosticum at the initial stage, the bacterial suspension was necessarily mixed and the optical concentration was measured photometrically. The concentration of the surfactant solution was adjusted to 25 ml of microbial cells in 1 ml. At the second stage, antigen-sensitin was obtained and stored at 4 ° C. Sheep erythrocytes were used for sensitization. At the third stage, 20% formalized ram erythrocytes were obtained. Formalized erythrocytes were washed five times until the supernatant was completely cleared and sensitized. At the fourth stage, 300-500 ml was added to 1 liter of erythrocyte suspension for sensitization. sensitin and kept in a water bath at a temperature of 600С. At the fifth stage, the sensitized erythrocytes were washed to remove residual sensitin not associated with erythrocytes. Obtaining highly effective erythrocyte diagnostics for the diagnosis of avian pullorosis typhoid is an urgent problem. We have produced, improved and optimized the technology of industrial production of erythrocyte pullor antigen from the Salmonella pullorum-gallinarum strain for the diagnosis of avian pullorosis-typhus. We have theoretically substantiated and tested in production conditions a new method of resuspension, extraction, clarification of the bacterial mass. Used surfactants (surfactants) to obtain sensitin.