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The effectiveness of the use of feed hydrolyzate in the poultry diet 饲料水解物在家禽日粮中的应用效果
Pub Date : 2021-06-01 DOI: 10.30917/ATT-VK-1814-9588-2021-3-5
E. S. Eganjan, A. Shantyz, Y. Lysenko, A.Yu. Shantyz, E. Marchenko
The authors of this article evaluated the effectiveness of the use of a feed additive based on soy protein hydrolysate in combination with iodine and selenium on laying hens of the Ross Hysex Brown cross. During the experiment, the following indicators were studied: safety, live weight gain, egg production, morpho-biochemical composition of blood. At the end of the experiment, the following data were obtained: the survival rate of the bird in the experimental group was 93,3 %, and in the control group the result was 86,6 %. The weight gain of laying hens in the experimental group increased by 11 %, the egg weight (average value) by 6,5 g (12,8 %), and in the control the increase was 2,7 g (5,2 %). In the experimental group, eggs per hen were obtained by 1,87% more than in the control group, in general, the experimental group showed an increase in the number of eggs during the experiment by 23,0 %. According to the results of the morphological and biochemical composition of the blood serum of birds, there are no significant negative changes. Red blood cells did not exceed intraspecific norms, but increased in the experimental group by 26,4 %. The amount of hemoglobin in the experimental group exceeded the control by 19,4 %. White blood cells in the experimental group were 16,9 % higher than this indicator in the control group. Biochemical analysis revealed a positive trend, which was expressed in an increase in the concentration of protein in the blood of chickens of the experimental group by 8,7 %, the level of calcium by 10,9 % and phosphorus by 31,3 % in birds of the experimental group exceeds the control.
本文的作者评估了以大豆蛋白水解物为基础的饲料添加剂与碘和硒联合使用对罗斯-海塞克斯-布朗杂交蛋鸡的有效性。实验期间,研究了以下指标:安全性、活重增加、产蛋量、血液形态生化成分。实验结束时,获得了以下数据:实验组的鸟类存活率为93,3%,对照组的存活率为86,6%。试验组蛋鸡增重增加11%,蛋重(平均值)增加6.5克(12.8%),对照组增加2.7克(5.2%)。在实验组中,每只母鸡的蛋数比对照组多1.87%,总的来说,实验组在实验期间的蛋数增加了23.0%。根据鸟类血清的形态学和生化组成的结果,没有明显的负面变化。红细胞没有超过种内标准,但在实验组中增加了26.4%。实验组血红蛋白含量比对照组高19.4%。实验组的白细胞比对照组的这一指标高16,9%。生化分析显示,实验组鸡血液中蛋白质浓度比对照组增加8,7%,钙水平比对照组提高10,9%,磷水平比对照增加31,3%。
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引用次数: 0
Physicochemical properties of the new drug Penox-1 新药Penox-1的理化性质
Pub Date : 2021-06-01 DOI: 10.30917/ATT-VK-1814-9588-2021-3-3
A. Batyrova, M. S. Saypullaev
The physicochemical properties of the substances included in the composition of the new disinfectant Penox-1 have been studied. Assessment of its disinfecting properties of veterinary in-spection objects, with the study of solubility in water and sodium chloride, surface tension of solutions, stability and adhesion of foam on vertical and ceiling test surfaces in laboratory conditions. At the initial stage, the temperature dependence of the solubility of slaked lime in water was es-tablished. It was determined by the change in the activity of OH- ions as the temperature of the over-saturated solution of slaked lime increased. Distilled water was used as a solvent. The solubility of slaked lime was also studied by adding 1.0; 3.0; 5.0 and 10.0 % sodium chloride solution. The experi-ments were carried out according to the method "On the procedure for testing new disinfectants for veterinary practice (1987)". Assessment with the study of the surface tension of solutions, resistance and adhesion of foam was carried out at the Institute of Physics, DSCRAN, also according to the indi-cated method. As a result of the experiments, it was found that with an increase in water temperature, the sol-ubility of slaked lime in it decreases. Adding 1 and 3% sodium chloride solution to a 90 % solution of slaked lime, the solubility increases by 2.15 and 2.2 times, respectively. With an increase in sodium chloride content to 5%, the solubility of hydrated lime begins to decrease. The presence in the solution of 20 % slaked lime and 3 % sodium chloride solution, 5 % foaming agent (PO-6k) reduces surface ten-sion and increases foam stability. It was also found that the thickness of the foam layer depends on the type of surface. The critical thickness of the foam layer on vertical surfaces was from 1 to 4 cm, on the ceiling – from 2 to 7 cm. The stability of the foam depends on its concentration in the solution. The foam content up to 5–6 % in the solution increased the resistance on vertical surfaces up to 16 minutes, and on ceiling surfaces up to 18 minutes.
研究了新型消毒剂Penox-1的理化性质。评估其对兽医检查对象的消毒性能,研究其在水中和氯化钠中的溶解度、溶液的表面张力、泡沫在实验室条件下在垂直和天花板测试表面上的稳定性和粘附性。在初始阶段,建立了熟石灰在水中溶解度的温度依赖性。它是通过OH-离子活性随熟石灰过饱和溶液温度升高而变化来确定的。蒸馏水被用作溶剂。通过添加1.0,研究了熟石灰的溶解度;3.0;5.0和10.0%氯化钠溶液。试验按照“兽医用新型消毒剂试验规程(1987)”的方法进行。同样根据指定的方法,在DSCRAN物理研究所对溶液的表面张力、泡沫的电阻和粘附性进行了评估。实验结果表明,随着水温的升高,熟石灰在其中的溶解度降低。在90%的熟石灰溶液中加入1%和3%的氯化钠溶液,溶解度分别增加2.15倍和2.2倍。随着氯化钠含量增加到5%,熟石灰的溶解度开始降低。在20%熟石灰和3%氯化钠溶液、5%发泡剂(PO-6k)的溶液中的存在降低了表面张力并提高了泡沫的稳定性。还发现泡沫层的厚度取决于表面的类型。垂直表面上泡沫层的临界厚度为1至4厘米,天花板上为2至7厘米。泡沫的稳定性取决于其在溶液中的浓度。溶液中的泡沫含量高达5-6%,可使垂直表面的阻力增加16分钟,天花板表面的阻力提高18分钟。
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引用次数: 0
The problems of brucellosis of small ruminants in Dagestan Republic and its solutions 达吉斯坦共和国小反刍动物布鲁氏菌病问题及对策
Pub Date : 2021-06-01 DOI: 10.30917/ATT-VK-1814-9588-2021-3-11
M. Mikailov, S. Gunashev, E. Yanikova, A. A. Halikov, A. Gulieva, D. M. Ramazanova
Brucellosis continues to remain an acute problem of veterinary and medicine, causing enormous damage for the national economy and posing a serious danger for the population. The disease is widespread in countries of all continents, where the infection occurs mainly in cattle (cattle), sheep, goats, as well as in wild pigs, bison, elk, and hares. In many constituent entities of the Russian Federation, in particular in the Caspian region, the results of the fight against brucellosis in animals remain unsatisfactory, the incidence of people remains on a high level, which is associated with the insufficient effectiveness of the anti-brucellosis measures, in particular, the diagnosis of this disease. According to many researchers, the serological tests used in practice make it possible to identify no more than 70% of infected sheep, which makes it difficult to eradicate brucellosis infection disease in herds of small ruminants. For this purpose, 423 heads of small ruminants were examined on brucellosis by various serological tests (IHAT, SAT, CFT, RBT). The experience has shown the advantage of IHAT using the developed antigen in comparison with other methods. At the same time, in comparison with these reactions, IHAT additionally revealed to SAT - 64 (15.1%), CFT - 28 (6.6%), RBT - 35 (8.3%) positively reacting sheep. One of the most pressing issues associated with brucellosis is the possibility of contracting a person with this disease and its consequences. The analysis of the epizootic situation showed that in recent years in Dagestan Republic, a difficult situation has developed on this zooanthroponosis. The epidemiological situation is also alarming. In recent years, there has been a significant increase of the number of sick people from 110 in 2016 to 202 2019. Despite the ongoing preventive and anti-brucellosis measures, no significant improvement has been achieved over the past 5 years. It is not possible to predict its improvement without making adjustments in regulatory documents and introducing of the most effective diagnostic methods.
布鲁氏菌病仍然是兽医和医学的一个严重问题,对国民经济造成巨大损害,并对人民构成严重危险。该病在各大洲国家广泛传播,感染主要发生在牛、绵羊、山羊以及野猪、野牛、麋鹿和野兔中。在俄罗斯联邦的许多组成实体中,特别是在里海地区,防治动物布鲁氏菌病的结果仍然不令人满意,人的发病率仍然很高,这与防治布鲁氏菌病措施的效力不足有关,特别是对这种疾病的诊断。根据许多研究人员的说法,在实践中使用的血清学测试只能识别不超过70%的受感染绵羊,这使得在小反刍动物群中根除布鲁氏菌病感染变得困难。为此目的,通过各种血清学试验(IHAT、SAT、CFT、RBT)对423头小反刍动物进行了布鲁氏菌病检查。经验表明,与其他方法相比,IHAT使用开发的抗原具有优势。与此同时,与这些反应相比,IHAT还显示SAT - 64(15.1%)、CFT - 28(6.6%)、RBT - 35(8.3%)呈阳性反应。与布鲁氏菌病相关的最紧迫问题之一是使人感染这种疾病及其后果的可能性。对动物疫情的分析表明,近年来达吉斯坦共和国出现了动物人畜共患病的困难局面。流行病学情况也令人震惊。近年来,患病人数从2016年的110人大幅增加到2019年的202人。尽管正在采取预防和抗布鲁氏菌病措施,但在过去5年中没有取得重大改善。如果不对规范性文件进行调整,不引入最有效的诊断方法,就不可能预测其改善。
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引用次数: 0
Current concepts of the role of fiber in the bird 纤维在鸟类中作用的当前概念
Pub Date : 2021-06-01 DOI: 10.30917/ATT-VK-1814-9588-2021-3-12
T. Okolelova, S. Engashev
Realization of the genetic potential of poultry productivity in industrial poultry farming is achieved by creating optimal conditions for keeping poultry and providing it with compound feeds, balanced in terms of metabolic energy and a complex of nutrient, mineral and biologically active substances. Fiber has always been and remains among the standardized indicators in mixed feed for poultry, since its excessive amount in a number of feed products (hulled crops, bran, sunflower meal, cake, etc.) is associated with a reduced digestibility of nutrients and energy availability). It is believed that fiber maintains the balance of the intestinal microflora, stimulates the growth of the muscular stomach and motility of the digestive tract, being a natural sorbent, absorbs toxic substances and ensures normal digestion and absorption of nutrients in feed. Despite the fact that the presence of fiber in compound feed for poultry is inevitable, the opinion of experts on its benefits differs.On the one hand, leading breeding companies and scientists consider fiber to be a necessary component for the development of the gizzard in order to stimulate the consumption of compound feed. On the other hand, poultry nutritionists try not to overload diets with fiber, especially if they consist of components that are difficult to hydrolyze, as this reduces the energy value of the diet. In addition, wastes from flour and fat-and-oil industries are often associated with such disadvantages as increased bacterial contamination or mycotoxin contamination. There is another controversial point that different sources of fiber have different effects on the digestive system, which is associated with the presence of anti-nutritional factors in the feed that affect its solubility. The review of publications provides information on the role of insoluble fiber in the body of poultry and its traditional and alternative sources.
通过创造饲养家禽的最佳条件,并为其提供代谢能量平衡的复合饲料以及营养、矿物质和生物活性物质的复合体,可以实现工业化家禽养殖中家禽生产力的遗传潜力。纤维一直是并仍然是家禽混合饲料的标准化指标之一,因为它在许多饲料产品(去皮作物、麸皮、向日葵粉、蛋糕等)中的过量与营养物质的消化率和能量利用率降低有关。据信,纤维可以维持肠道菌群的平衡,刺激肌肉胃的生长和消化道的运动,是一种天然的吸附剂,可以吸收有毒物质,确保饲料中营养物质的正常消化和吸收。尽管纤维在家禽复合饲料中的存在是不可避免的,但专家们对其益处的看法各不相同。一方面,领先的养殖公司和科学家认为纤维是开发砂仁的必要成分,以刺激复合饲料的消费。另一方面,家禽营养学家尽量不让饮食中的纤维过多,尤其是如果它们含有难以水解的成分,因为这会降低饮食的能量值。此外,面粉和油脂工业产生的废物往往与细菌污染或真菌毒素污染增加等缺点有关。另一个有争议的观点是,不同来源的纤维对消化系统的影响不同,这与饲料中存在影响其溶解度的抗营养因子有关。出版物综述提供了关于不溶性纤维在家禽体内的作用及其传统和替代来源的信息。
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引用次数: 0
Control of chronic coronavirus infection in cats with the use of Phosprenyl and Gamavit 应用磷酸丙烯和加玛韦控制猫慢性冠状病毒感染
Pub Date : 2021-06-01 DOI: 10.30917/ATT-VK-1814-9588-2021-3-13
S. Savoyskaya, I. Ogorodnikova, T. Kozhevnikova
Feline enteropathogenic coronavirus affects the intestinal epithelium and causes inflammation of the intestinal mucosa in cats. In adult cats, the infection is usually asymptomatic, but the constant circulation of the coronavirus in the feline population can increase the likelihood of mutation leading to appearance of the virulent strain of the virus that cause feline infectious peritonitis - a deadly disease that develops in 5-10% of cats carrying the coronavirus. The aim of this work was to study the effect of the drugs Phosprenyl and Gamavit on the course of coronavirus infection in cats. A total of 29 cats were monitored. The diagnosis was confirmed by the detection of antibodies by the IHA method, as well as by ultrasound examination of the abdominal organs. Prescribed treatment: Phosprenyl per os, 1 ml , twice a day, course 2-4 months; Gamavit 1 time a day, s/c, course 10 days, with a repeated course after 3 weeks; 3-4 times. As symptomatic therapy, drugs were used to improve the condition of the stool. At the end of treatment, symptoms of gastrointestinal tract damage disappeared in all patients. If before the start of treatment, most patients showed signs of anemia (a decrease in the number of red blood cells and hemoglobin levels), leukocytosis with a predominance of segmented neutrophils, characteristic of infectious and inflammatory processes, and increased ESR, then at the end of therapy, these indicators were completely normalized. Thus, long-term therapy with the use of Phosprenyl and Gamavit helps to control the course of coronavirus infection in cats, prevent its exacerbation and transition to a highly lethal form of infectious peritonitis.
猫肠道致病性冠状病毒影响猫的肠上皮,并引起猫肠粘膜炎症。在成年猫中,感染通常是无症状的,但冠状病毒在猫群中的持续循环会增加突变的可能性,从而导致致命的病毒株出现,从而导致猫传染性腹膜炎,这种致命的疾病会在5-10%携带冠状病毒的猫身上发生。本工作的目的是研究药物Phospreny和Gamavit对猫冠状病毒感染过程的影响。共监测了29只猫。通过IHA法检测抗体以及腹部器官的超声检查证实了诊断。处方治疗:Phosprenyl每次口服,1毫升,每天两次,疗程2-4个月;Gamavit每天1次,s/c,疗程10天,3周后重复疗程;3-4次。作为症状治疗,药物被用来改善大便的状况。治疗结束时,所有患者的胃肠道损伤症状均消失。如果在治疗开始前,大多数患者表现出贫血(红细胞数量和血红蛋白水平下降)、白细胞增多(以分段中性粒细胞为主)、感染和炎症过程特征以及ESR增加的迹象,那么在治疗结束时,这些指标完全正常化。因此,使用Phospreny和Gamavit的长期治疗有助于控制猫的冠状病毒感染过程,防止其恶化并转变为高度致命的感染性腹膜炎。
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引用次数: 1
Causative agents of campylo-bacteriosis of birds – etiological factors of toxicoinfections in humans 鸟类弯曲菌病的病原——人中毒感染的病原学因素
Pub Date : 2021-06-01 DOI: 10.30917/ATT-VK-1814-9588-2021-3-15
A. Sukhinin, T. Rozhdestvenskaya, S. V. Pankratov, L. Smirnova, S. Makavchik
This article is devoted to the problems of the emergence among the population of acute intestinal infectious diseases of a zoonotic nature caused by campylobacteriosis. According to WHO estimates, for many years the dominant cause in the development of acute intestinal infectious diseases in humans of a zoonotic nature is campylobacteriosis, which has recently surpassed salmonellosis in prevalence. Campylobacteriosis is a zoonotic infectious disease of agricultural, domestic and wild birds, animals and humans, caused by microorganisms of the genus Campylobacter, characterized by varying degrees of severity and polymorphism manifestations. Bacteria of the Campylobacter genus are ubiquitous in nature, they are present in the body of poultry and animals. The main reservoirs of Campylobacter are wild and domestic birds, primarily chickens. The high infection rate of C. jejuni of poultry flocks leads to more frequent detection of these bacteria in various types of poultry products. As a result, raw materials and poultry products are considered the main source of excretion and transmission factor of campylobacteriosis pathogens, posing the greatest risk to human health, since chicken meat and eggs occupy one of the dominant places in the population's diet. A high percentage of C. jejuni bacteria carriers among poultry, in case of violation of veterinary and sanitary measures in the farm and against the background of other latent infections, can cause significant economic damage to poultry farming due to a decrease in egg production, weight, mortality of poultry and an increase in the cost of health measures. Today, the system for controlling the spread of campylobacteriosis in poultry farms and at poultry processing enterprises is based on the use of general veterinary-zoo hygienic and antiepizootic measures, which does not always prevent infection of poultry, poultry products and, accordingly, people. In this regard, the development of a National Program for the Control of Campylobacteriosis in Poultry Farms of the Russian Federation in order to ensure the epidemiological well-being of the population is relevant.
本文致力于解决由弯曲杆菌病引起的人畜共患性急性肠道传染病在人群中出现的问题。根据世界卫生组织的估计,多年来,人畜共患急性肠道传染病的主要原因是弯曲杆菌病,该病的流行率最近超过了沙门氏菌病。弯曲杆菌病是由弯曲杆菌属微生物引起的一种人畜共患的农业、家庭和野生鸟类、动物和人类传染病,具有不同程度的严重性和多态性表现。弯曲杆菌属细菌在自然界中普遍存在,它们存在于家禽和动物体内。弯曲杆菌的主要宿主是野生鸟类和家禽,主要是鸡。鸡群空肠弯曲菌的高感染率导致在各种类型的家禽产品中更频繁地检测到这些细菌。因此,原材料和家禽产品被认为是弯曲杆菌病病原体排泄和传播的主要来源,对人类健康构成最大风险,因为鸡肉和鸡蛋在人群饮食中占据主导地位。家禽中空肠弯曲菌携带者的比例很高,如果违反农场的兽医和卫生措施,并在其他潜在感染的背景下,由于鸡蛋产量、重量、家禽死亡率的下降和卫生措施成本的增加,可能会对家禽养殖造成重大经济损失。如今,控制弯曲杆菌病在家禽养殖场和家禽加工企业传播的系统是基于使用一般兽医动物园卫生和抗寄生虫措施,这并不总是能预防家禽、家禽产品以及相应的人的感染。在这方面,制定俄罗斯联邦家禽养殖场弯曲杆菌病控制国家方案,以确保人口的流行病学健康是相关的。
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引用次数: 0
The quality of the silage when preserving the green mass of herbs with a new generation of leavens in laboratory conditions 在实验室条件下用新一代酵母保存草本植物的青贮质量
Pub Date : 2021-06-01 DOI: 10.30917/ATT-VK-1814-9588-2021-3-8
Y. Kravainis, R. Kravaine, A. Konovalov, А.А. Alekseev, А.A. Payuta
The article presents the results of studies of the quality of silage in the canning of cere-al-legume green mass: the leaven "Yarosil, bacterial preservative, suspension" in an amount: 50 ml per ton, 275 ml per ton 500 ml per ton, containing Lactobacillus plantarum K-36, K- 64, K-65, K-72, K-74, K-87, K-167; Lactobacillus lactis subsp. lactis Ep-157, Lactobacillus paracasei Ep-154 Ep-155 Ep-156 Ep-158, Streptococcus thermophilus K-35, the concentration (total) was 1.5õ109 CFU / cm3; starter culture "Yarosil dry powder" in a dose: 1 g per ton, 5.5 g per ton, 10 g per ton The composition of the microflora was the same as in the yeast "Yarosil, bacterial preservative, suspension", but the concentration was different and amounted to "Yarosil dry powder" (total) 8õ1010 CFU / cm3. The optimal amount of introduction (dose) of the indi-cated starter cultures into the canned green cereal-legume mass has been revealed. The amount of sourdough added had an impact on the safety, chemical composition and nutritional value of the resulting haylage. More optimal results were obtained when adding the starter culture "Yar-osil bacterial preservative, suspension" in the amount of 50 ml / t to the cereal-legume green mass. The starter culture increased the crude protein content by 1.03% (the control sample con-tained 7.85%, the experimental one - 8.88%), reduced the crude fiber content by 0.74% (the control sample contained 27.13%, the experimental – 26, 39%), reduced the content of butyric acid by 0.11% in the control sample contained 0.17%, in the experimental - 0,06%). When add-ing the starter culture "Yarosil dry powder" the best result was obtained when adding 5.5 g/ t. The starter culture increased the crude protein content by 1.54% (the control sample contained 7.85%, the experimental one – 9.39 %), reduced the crude fiber content by 0.72 % (the control sample contained 27.13 %, the experimental – 26, 41%), reduced the content of butyric acid by 0.06 % in the control sample contained 0.17%, in the experimental – 0,11 %). The amount of the added starter culture did not affect the organoleptic characteristics. The resulting haylage corresponded to GOST R 55986-2014, no mold or rot was found in it, the crushed mass of plants (stems, leaves) was clearly distinguishable, had a greenish-yellow color, a pleasant, specific smell of pickled vegetables. As a result of the research, the optimal doses of the studied starter cultures were established for the quality of haylage from cereal-leguminous green mass.
本文介绍了豆科绿块状罐头青贮质量的研究结果:酵母“雅罗什尔、细菌保鲜剂、悬浮液”的用量:每吨50 ml、每吨275 ml、每吨500 ml,含植物乳杆菌K-36、K- 64、K-65、K-72、K-74、K-87、K-167;乳酸乳杆菌亚种乳酸菌Ep-157、副干酪乳杆菌Ep-154 Ep-155 Ep-156 Ep-158、嗜热链球菌K-35,浓度(总)为1.5õ109 CFU / cm3;发酵剂“雅罗斯尔干粉”的剂量为:1 g /吨、5.5 g /吨、10 g /吨。菌群组成与酵母“雅罗斯尔、细菌防腐剂、悬浮液”相同,但浓度不同,达“雅罗斯尔干粉”(总量)8õ1010 CFU / cm3。揭示了绿色谷物豆类罐头中指定发酵剂的最佳引入量(剂量)。酵母的添加量对所得牧草的安全性、化学成分和营养价值都有影响。在谷物豆科绿块中添加50ml / t的发酵剂“石蜡菌防腐剂悬浮液”效果更优。发酵剂使粗蛋白质含量提高1.03%(对照7.85%,试验组- 8.88%),粗纤维含量降低0.74%(对照27.13%,试验组- 26.39%),丁酸含量降低0.11%(对照0.17%,试验组- 0.06%)。当添加“雅罗西尔干粉”发酵剂时,以添加量为5.5 g/ t时效果最佳,发酵剂可使粗蛋白质含量提高1.54%(对照为7.85%,试验组为- 9.39%),粗纤维含量降低0.72%(对照为27.13%,试验组为- 26.41%),丁酸含量降低0.06%(对照为0.17%,试验组为- 0.11%)。发酵剂的添加量对其感官特性没有影响。所得干草符合GOST R 55986-2014,未发现霉变或腐烂,粉碎的植物(茎、叶)清晰可辨,呈黄绿色,有一种宜人的腌制蔬菜的特有气味。通过研究,确定了所研究的发酵剂的最佳剂量,以提高禾豆青块牧草的品质。
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引用次数: 0
The effect of vitamin and mineral supplements on the replication of coronaviruses in the SPEV cell culture 补充维生素和矿物质对冠状病毒在SPEV细胞培养中复制的影响
Pub Date : 2021-06-01 DOI: 10.30917/ATT-VK-1814-9588-2021-3-10
V. Laskavyy, T. Polyanina, V. Laskavaya, V. T. Nochevny
The article describes studies on the effect of vitamin and mineral supplements (VMS) on the replication of coronavirus in tissue culture. The cytopathic effect of the porcine transmissible gastroenteritis virus (strain TO36SD192 (Japan) and the vaccine strain "RIMS" was studied on the SPEV cell line in the presence of mineral and vitamin supplements (VMS) with an increased content of vitamins A and D. The results of the study showed that the introduction of vitamin and mineral supplements into the growth medium provides an increase in the yield of the RIMS virus by 16.7 - 23%. Interestingly, at the minimum infectious dose, the presence or absence of VMS does not in any way affect the titer of the virus of the reference strain of the coronavirus TO36SD192. However, an increase in the infectious dose by only one order of magnitude (1000 TDC50 / ml) stimulates an increase in the virus titer by 22.5% in the presence of VMS. With the addition of VMS, the RIMS strain reproduced more actively and the virus titer in the experiment with the addition of VMS was 30% higher than in the control (without VMS). A further increase in the infectious dose showed a significant increase in the virus titer from 13.4% to 6.25%. It should be noted that the TO36SD192 virus, well adapted to the SPEV culture, can be compared with viruses that cause seasonal ARVI in humans, which are also well adapted in the human body, and the use of VMS during the peak of the epidemic can stimulate viral replication. Since the RIMS strain virus is poorly adapted to the SPEV culture, its action can be compared with a virus that has entered the human population, for example, SARS-CoV-2. This means that taking vitamins at any infectious dose (during an epidemic) will stimulate the disease.
本文介绍了维生素和矿物质补充剂(VMS)对冠状病毒组织培养复制影响的研究。研究了猪传染性胃肠炎病毒(日本TO36SD192株)和疫苗株“RIMS”在增加维生素A和d含量的矿物质和维生素补充剂(VMS)存在下对SPEV细胞系的细胞病变作用。研究结果表明,在生长培养基中添加维生素和矿物质补充剂可使RIMS病毒的产量增加16.7% ~ 23%。有趣的是,在最低感染剂量下,VMS的存在或不存在不会以任何方式影响冠状病毒参考株TO36SD192的病毒滴度。然而,在VMS存在的情况下,感染剂量仅增加一个数量级(1000 TDC50 / ml)就会刺激病毒滴度增加22.5%。添加VMS后,RIMS菌株繁殖更活跃,实验中添加VMS的病毒滴度比不添加VMS的病毒滴度高30%。进一步增加感染剂量,病毒滴度从13.4%显著增加到6.25%。值得注意的是,TO36SD192病毒对SPEV培养物适应良好,可与引起人类季节性ARVI的病毒进行比较,后者在人体中也适应良好,在疫情高峰期使用VMS可刺激病毒复制。由于RIMS毒株对SPEV培养物的适应性较差,因此其作用可与进入人群的病毒(例如SARS-CoV-2)进行比较。这意味着服用任何传染性剂量的维生素(在流行病期间)都会刺激疾病。
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引用次数: 0
Milk productivity of Yakut horses 雅库特马的产奶量
Pub Date : 2021-06-01 DOI: 10.30917/ATT-VK-1814-9588-2021-3-6
R. Ivanov, M. Pak, V. Osipov, U. Hompodoeva, D. N. Sourdin, E. Sleptsov
The article presents a brief review of scientific research on the milk productivity of mares of the Yakut breed. One of the most valuable products of horse breeding in Yakutia is the milk of Yakut mares, which is characterized by a high content of milk sugar, rich in vitamin C, which is especially important for the production of koumiss. Milk of mares of the Yakut horse breed is a valuable raw material for processing. A brief review of research has shown that the mares of Bashkir, Kazakh and Kirghiz breeds, which have been milking since ancient times, have a good milk content among the horse breeds of the herd. A Mare of the Yakut horse breed gives an average of 4 – 5 liters per day, and a maximum of 6 – 8 liters of milk. According To M. F. Gabyshev's research, the average daily milk productivity of a Mare reaches 10–12 liters. A thorough study of milk production of mares of the Yakut breed held the T. V. Amosova. She found that for 6 months of lactation, the daily milk yield of mares of the Yakut breed averaged 9.9 liters, during this time they produce from 1124 to 1942 liters, an average of 1750 liters of milk. In studies A. I. Pavlova found that the native type mares of the Yakut breed of horses milk yield is 8.8 l per day and 1592 L. 6 months of lactation and in enlarged type – 9.2 and 1666 l; from MagicScore and 13.3 2393 and l, respectively. It was found that winter milk is inferior to summer milk in terms of protein, ash, phosphorus, calcium, and vitamin C, but it is higher in sugar content and has a high acidity. As a result, we came to the conclusion that it is necessary: 1. conduct breeding work to increase the milk productivity of mares of the Yakut horse breed, 2.develop and test a recipe for starter feed for weanling foals that is similar in composition to their mothers' milk.
本文简要介绍了雅库特品种母马产奶能力的科学研究进展。雅库特最有价值的马匹养殖产品之一是雅库特母马的牛奶,其特点是含有高含量的奶糖,富含维生素C,这对koumiss的生产尤为重要。雅库特马种母马的乳汁是一种很有价值的加工原料。对研究的简要回顾表明,巴什基尔、哈萨克和吉尔吉斯品种的母马自古以来就在挤奶,在牛群的马种中,它们的乳汁含量很高。雅库特马品种的母马平均每天产奶4-5升,最多产奶6-8升。根据M.F.Gabyshev的研究,母马的平均日产奶量达到10-12升。对雅库特品种母马产奶量的深入研究使阿莫索娃获得了T.V。她发现,在6个月的哺乳期内,雅库特品种母马的平均日产奶量为9.9升,在此期间,母马的日产奶量从1124升到1942升,平均为1750升。A.I.巴甫洛娃在研究中发现,雅库特品种马的本地型母马日产奶量为8.8升,泌乳期为1592升。6个月,扩大型为9.2升和1666升;分别来自MagicScore和13.32393和l。研究发现,冬奶在蛋白质、灰分、磷、钙和维生素C方面不如夏奶,但含糖量较高,酸度较高。因此,我们得出的结论是,这是必要的:1。进行育种工作以提高雅库特马品种母马的产奶能力。2.开发并测试断奶小马驹的起始饲料配方,该配方与母马的乳汁成分相似。
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引用次数: 0
Clinical morphological reasoning of the diagnosis of subclinical cows endometritis 奶牛亚临床子宫内膜炎诊断的临床形态学依据
Pub Date : 2021-06-01 DOI: 10.30917/ATT-VK-1814-9588-2021-3-14
N. Slesarenko, E. Shirokova, A. P. Belyakova
The project presents the results of the clinical and morphological substantiation of the diagnosis of endometritis in cows. The scientific and production part of the experiment was carried out by the method of selection of analog groups, taking into account the reproductive age and live weight, according to generally accepted methods. Two groups of animals were formed. Group one - cows with subclinical endometritis, group two - with no signs of this pathology, which is morphological control. Of these, seven animals were healthy and thirteenth with signs of endometritis. Of these, pathology in an acute form was detected in three animals, in a latent form in ten animals. The formation of a group of animals with a normal course of the postpartum period was carried out on the basis of techniques, including examination of the external genitalia and rectal examinations. General clinical indicators were also taken into account: temperature, pulse and respiration. The studies were carried out on the basis of the Department of Anatomy and Histology of Animals named after Professor A.F. Klimov FGBOU VO MGAVMiB - MBA named after K.I. Scriabin, as well as in ZAO Povadino breeding plant. Hematological studies were carried out on the basis of the Chance Bio laboratory. A comprehensive methodological approach was used in the work, including clinical examination of animals, macroscopic morphometry, anatomical preparation, morphological blood tests, statistical analysis of the obtained digital data. The normative morphological parameters of the uterus in black-white Holsteinized cows were established as the baseline for assessing the nature of structural changes in the organ with subclinical endometritis. Revealed pathognomonic morphological signs of the uterus in subclinical endometritis, expressed in an increase in its cavity and horns, the accumulation of exudate in them, in the swelling and dullness of the endometrium. In the course of the conducted hematological studies, the manifestation of an inflammatory reaction was noted, expressed in an increase in the number of leukocytes, stab and segmented neutrophils and monocytes. The article presents the clinical and morphological substantiation of the diagnosis of subclinical endometritis, based on a comprehensive assessment of changes in the morphometric parameters of the uterus and hematological changes occurring in the body.
该项目提出了奶牛子宫内膜炎诊断的临床和形态学证实的结果。试验的科学和生产部分,参照育龄和活重,按普遍接受的方法选择模拟组进行。于是形成了两组动物。第一组是患有亚临床子宫内膜炎的奶牛,第二组是没有这种病理症状的奶牛,这是形态学控制。其中,7只动物是健康的,13只有子宫内膜炎的迹象。其中,在3只动物中检测到急性病理形式,在10只动物中检测到潜伏形式。在技术基础上进行了一组具有正常产后病程的动物,包括外生殖器检查和直肠检查。一般临床指标也被考虑在内:体温、脉搏和呼吸。这些研究是在以A.F. Klimov教授命名的动物解剖和组织学部门FGBOU VO MGAVMiB - MBA以K.I. Scriabin命名,以及在ZAO Povadino育种工厂进行的。血液学研究是在Chance Bio实验室的基础上进行的。在工作中采用了全面的方法学方法,包括动物临床检查,宏观形态测量,解剖准备,形态学血液检查,对获得的数字数据进行统计分析。建立了黑白荷斯坦奶牛子宫的规范形态学参数,作为评估亚临床子宫内膜炎器官结构变化性质的基线。揭示了亚临床子宫内膜炎的病理形态学征象,表现为子宫腔和角的增加,其中积存渗出物,子宫内膜肿胀和暗沉。在进行血液学研究的过程中,注意到炎症反应的表现,表现为白细胞、刺状和分节性中性粒细胞和单核细胞数量的增加。本文介绍了诊断亚临床子宫内膜炎的临床和形态学依据,基于对子宫形态参数变化和体内血液学变化的综合评估。
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引用次数: 0
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Athletic Therapy Today
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