Effects of Meloidogyne incognita, Pseudomonas syringae pv. pisi and Rhizobium leguminosarum inoculated alone, simultaneously, and sequentially, on the growth and biochemical parameters of pea (Pisum sativum) in three soil types

Abstract

Effects of Meloidogyne incognita, Pseudomonas syringae pv. pisi and Rhizobium leguminosarum were studied on growth and biochemical parameters of pea (Pisum sativum L.) in three soil types. Plants grown in 20% fly ash attained higher plant growth, chlorophyll and carotenoid followed by loam soil and 20% sand. Inoculation of R. leguminosarum resulted in increased plant growth, nodulation, chlorophyll and carotenoid over control. Root nodulation and proline contents were high in plants grown in 20% sand and least in 20% fly ash. Inoculation of M. incognita prior to P. syringae pv. pisi resulted in a greater reduction in plant growth, nodulation, chlorophyll and carotenoid content and least where P. syringae pv. pisi was inoculated prior to M. incognita. Inoculation of pathogens increased proline contents. Galling and population of M. incognita was high in 20% sand followed by loam soil and fly ash amended soil. P. syringae pv. pisi and R. leguminosarum had adverse effect on galling and nematode population. The principal component analysis identifies interaction of pathogens and showed segregation of various treatments in the plots.