Easun Arunachalam, W. Ireland, Xingbo Yang, D. Needleman
{"title":"Dissecting Flux Balances to Measure Energetic Costs in Cell Biology: Techniques and Challenges","authors":"Easun Arunachalam, W. Ireland, Xingbo Yang, D. Needleman","doi":"10.1146/annurev-conmatphys-031620-105251","DOIUrl":null,"url":null,"abstract":"Life is a nonequilibrium phenomenon: Metabolism provides a continuous supply of energy that drives nearly all cellular processes. However, very little is known about how much energy different cellular processes use, i.e., their energetic costs. The most direct experimental measurements of these costs involve modulating the activity of cellular processes and determining the resulting changes in energetic fluxes. In this review, we present a flux balance framework to aid in the design and interpretation of such experiments and discuss the challenges associated with measuring the relevant metabolic fluxes. We then describe selected techniques that enable measurement of these fluxes. Finally, we review prior experimental and theoretical work that has employed techniques from biochemistry and nonequilibrium physics to determine the energetic costs of cellular processes. Expected final online publication date for the Annual Review of Condensed Matter Physics, Volume 14 is March 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.","PeriodicalId":7925,"journal":{"name":"Annual Review of Condensed Matter Physics","volume":" ","pages":""},"PeriodicalIF":14.3000,"publicationDate":"2022-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annual Review of Condensed Matter Physics","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1146/annurev-conmatphys-031620-105251","RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PHYSICS, CONDENSED MATTER","Score":null,"Total":0}
引用次数: 1
Abstract
Life is a nonequilibrium phenomenon: Metabolism provides a continuous supply of energy that drives nearly all cellular processes. However, very little is known about how much energy different cellular processes use, i.e., their energetic costs. The most direct experimental measurements of these costs involve modulating the activity of cellular processes and determining the resulting changes in energetic fluxes. In this review, we present a flux balance framework to aid in the design and interpretation of such experiments and discuss the challenges associated with measuring the relevant metabolic fluxes. We then describe selected techniques that enable measurement of these fluxes. Finally, we review prior experimental and theoretical work that has employed techniques from biochemistry and nonequilibrium physics to determine the energetic costs of cellular processes. Expected final online publication date for the Annual Review of Condensed Matter Physics, Volume 14 is March 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.
期刊介绍：
Since its inception in 2010, the Annual Review of Condensed Matter Physics has been chronicling significant advancements in the field and its related subjects. By highlighting recent developments and offering critical evaluations, the journal actively contributes to the ongoing discourse in condensed matter physics. The latest volume of the journal has transitioned from gated access to open access, facilitated by Annual Reviews' Subscribe to Open initiative. Under this program, all articles are now published under a CC BY license, ensuring broader accessibility and dissemination of knowledge.
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