Hemocyte proteome of the Lake Baikal endemic Eulimnogammarus verrucosus (Crustacea: Amphipoda) sheds light on immune-related proteins

Q3 Agricultural and Biological Sciences Biological Communications Pub Date : 2021-12-30 DOI:10.21638/spbu03.2021.402
E. Zolotovskaya, A. Nazarova, A. Saranchina, A. Mutin, P. Drozdova, Y. Lubyaga, M. Timofeyev
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引用次数: 1

Abstract

Hemocytes are cells circulating in the hemolymph and playing an important role in crustacean immunity. These cells not only function as phagocytes but also express immune compounds to the hemolymph. Here we obtained hemocyte proteome of the endemic amphipod (Amphipoda, Crustacea) Eulimnogammarus verrucosus from Lake Baikal, the first hemocyte proteome of an amphipod, using liquid chromatography/tandem mass spectrometry (LC-MS/MS). A total of 1152 unique proteins were discovered with LC-MS/MS. We discovered both proteins directly involved in the immune response, such as pattern recognition proteins (C-type lectins), and compounds with antimicrobial activity (ctenidin and anti-lipopolysaccharide factor/scygonadin). Moreover, hemocyanins which may act as a phenoloxidase and C-type lectins were among the most diverse protein groups in the hemocyte proteome. The obtained data can be useful for further studies of immune components and mechanisms in Baikal amphipods.
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贝加尔湖地方性疣状Eulimnogamarus(甲壳纲:两栖纲)的血细胞蛋白质组揭示了免疫相关蛋白
血细胞是在血淋巴中循环的细胞,在甲壳类动物的免疫中起着重要作用。这些细胞不仅具有吞噬细胞的功能,而且还向血淋巴表达免疫化合物。在这里,我们使用液相色谱/串联质谱法(LC-MS/MS)获得了来自贝加尔湖的地方性两栖动物(两栖纲,甲壳纲)疣状Eulimnogamarus的血细胞蛋白质组,这是第一个两栖动物的血细胞蛋白组。LC-MS/MS共发现1152种独特的蛋白质。我们发现了两种直接参与免疫反应的蛋白质,如模式识别蛋白(C型凝集素)和具有抗菌活性的化合物(ctenidin和抗脂多糖因子/奇果纳丁)。此外,可以作为酚氧化酶的血蓝蛋白和C型凝集素是血细胞蛋白质组中最多样化的蛋白质组之一。所获得的数据可用于贝加尔湖片脚类动物免疫成分和机制的进一步研究。
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来源期刊
Biological Communications
Biological Communications Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.70
自引率
0.00%
发文量
21
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