{"title":"Overcoming Major Barriers to Developing Successful Sensors for Practical Applications Using Functional Nucleic Acids.","authors":"Jingjing Zhang, Tian Lan, Yi Lu","doi":"10.1146/annurev-anchem-061020-104216","DOIUrl":null,"url":null,"abstract":"For many years, numerous efforts have been focused on the development of sensitive, selective, and practical sensors for environmental monitoring, food safety, and medical diagnostic applications. However, the transition from innovative research to commercial success is relatively sparse. In this review, we identify several barriers to developing successful sensors for practical applications, including the lack of general methods to (a) generate receptors for a wide range of targets, (b) improve sensor selectivity to overcome interferences, (c) transduce the selective binding to different optical, electrochemical, and other signals, and (d) tune dynamic range to match thresholds of detection required for different targets. We then summarize solutions to overcome these barriers using sensors based on functional nucleic acids that include DNAzymes, aptamers, and aptazymes and how these sensors are coupled to widely available measurement devices to expand their capabilities and lower the barrier for their practical applications in the field and point-of-care settings. Expected final online publication date for the Annual Review of Analytical Chemistry Volume 15 is June 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.","PeriodicalId":50758,"journal":{"name":"Annual Review of Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":5.9000,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annual Review of Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1146/annurev-anchem-061020-104216","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 5
Abstract
For many years, numerous efforts have been focused on the development of sensitive, selective, and practical sensors for environmental monitoring, food safety, and medical diagnostic applications. However, the transition from innovative research to commercial success is relatively sparse. In this review, we identify several barriers to developing successful sensors for practical applications, including the lack of general methods to (a) generate receptors for a wide range of targets, (b) improve sensor selectivity to overcome interferences, (c) transduce the selective binding to different optical, electrochemical, and other signals, and (d) tune dynamic range to match thresholds of detection required for different targets. We then summarize solutions to overcome these barriers using sensors based on functional nucleic acids that include DNAzymes, aptamers, and aptazymes and how these sensors are coupled to widely available measurement devices to expand their capabilities and lower the barrier for their practical applications in the field and point-of-care settings. Expected final online publication date for the Annual Review of Analytical Chemistry Volume 15 is June 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.
期刊介绍:
The Annual Review of Analytical Chemistry, launched in 2008, offers a comprehensive perspective on the field, drawing from diverse disciplines such as biology, physics, and engineering, with analytical chemistry as the central theme. The journal's current volume has transitioned from gated to open access through Annual Reviews' Subscribe to Open program, with all articles published under a CC BY license.