Pub Date : 2024-05-03DOI: 10.1146/annurev-anchem-061622-012448
Malama Chisanga, Jean-Francois Masson
A frontier of analytical sciences is centered on the continuous measurement of molecules in or near cells, tissues, or organs, within the biological context in situ, where the molecular-level information is indicative of health status, therapeutic efficacy, and fundamental biochemical function of the host. Following the completion of the Human Genome Project, current research aims to link genes to functions of an organism and investigate how the environment modulates functional properties of organisms. New analytical methods have been developed to detect chemical changes with high spatial and temporal resolution, including minimally invasive surface-enhanced Raman scattering (SERS) nanofibers using the principles of endoscopy (SERS nanoendoscopy) or optical physiology (SERS optophysiology). Given the large spectral data sets generated from these experiments, SERS nanoendoscopy and optophysiology benefit from advances in data science and machine learning to extract chemical information from complex vibrational spectra measured by SERS. This review highlights new opportunities for intracellular, extracellular, and in vivo chemical measurements arising from the combination of SERS nanosensing and machine learning.
{"title":"Machine Learning–Driven SERS Nanoendoscopy and Optophysiology","authors":"Malama Chisanga, Jean-Francois Masson","doi":"10.1146/annurev-anchem-061622-012448","DOIUrl":"https://doi.org/10.1146/annurev-anchem-061622-012448","url":null,"abstract":"A frontier of analytical sciences is centered on the continuous measurement of molecules in or near cells, tissues, or organs, within the biological context in situ, where the molecular-level information is indicative of health status, therapeutic efficacy, and fundamental biochemical function of the host. Following the completion of the Human Genome Project, current research aims to link genes to functions of an organism and investigate how the environment modulates functional properties of organisms. New analytical methods have been developed to detect chemical changes with high spatial and temporal resolution, including minimally invasive surface-enhanced Raman scattering (SERS) nanofibers using the principles of endoscopy (SERS nanoendoscopy) or optical physiology (SERS optophysiology). Given the large spectral data sets generated from these experiments, SERS nanoendoscopy and optophysiology benefit from advances in data science and machine learning to extract chemical information from complex vibrational spectra measured by SERS. This review highlights new opportunities for intracellular, extracellular, and in vivo chemical measurements arising from the combination of SERS nanosensing and machine learning.","PeriodicalId":50758,"journal":{"name":"Annual Review of Analytical Chemistry","volume":"43 1","pages":""},"PeriodicalIF":8.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140835341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The last decade has been incredibly fruitful in proving the multifunctionality of paper for delivering innovative electrochemical (bio)sensors. The paper material exhibits unprecedented versatility to deal with complex liquid matrices and facilitate analytical detection in aerosol and solid phases. Such remarkable capabilities are feasible by exploiting the intrinsic features of paper, including porosity, capillary forces, and its easy modification, which allow for the fine designing of a paper device. In this review, we shed light on the most relevant paper-based electrochemical (bio)sensors published in the literature so far to identify the smart functional roles that paper can play to bridge the gap between academic research and real-world applications in the biomedical, environmental, agrifood, and security fields. Our analysis aims to highlight how paper's multifarious properties can be artfully harnessed for breaking the boundaries of the most classical applications of electrochemical (bio)sensors.
{"title":"Paper-Based Electrochemical (Bio)Sensors for the Detection of Target Analytes in Liquid, Aerosol, and Solid Samples","authors":"Noemi Colozza, Vincenzo Mazzaracchio, Fabiana Arduini","doi":"10.1146/annurev-anchem-061522-034228","DOIUrl":"https://doi.org/10.1146/annurev-anchem-061522-034228","url":null,"abstract":"The last decade has been incredibly fruitful in proving the multifunctionality of paper for delivering innovative electrochemical (bio)sensors. The paper material exhibits unprecedented versatility to deal with complex liquid matrices and facilitate analytical detection in aerosol and solid phases. Such remarkable capabilities are feasible by exploiting the intrinsic features of paper, including porosity, capillary forces, and its easy modification, which allow for the fine designing of a paper device. In this review, we shed light on the most relevant paper-based electrochemical (bio)sensors published in the literature so far to identify the smart functional roles that paper can play to bridge the gap between academic research and real-world applications in the biomedical, environmental, agrifood, and security fields. Our analysis aims to highlight how paper's multifarious properties can be artfully harnessed for breaking the boundaries of the most classical applications of electrochemical (bio)sensors.","PeriodicalId":50758,"journal":{"name":"Annual Review of Analytical Chemistry","volume":"2 1","pages":""},"PeriodicalIF":8.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140628687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-19DOI: 10.1146/annurev-anchem-061522-040824
Barry L. Smith, Thomas Hankinson, Simon Maher
We critically evaluate the current status of portable mass spectrometry (pMS), particularly where this aligns with ambient ionization. Assessing the field of pMS can be quite subjective, especially in relation to the portable aspects of design, deployment, and operation. In this review, we discuss what it means to be portable and introduce a set of criteria by which pMS and ambient ionization sources can be assessed. Moreover, we consider the recent literature in terms of the most popular and significant advances in portable instrumentation for ambient ionization and miniature mass spectrometers. Finally, emerging trends and exciting future prospects are discussed and some recommendations are offered.
{"title":"Portable Instrumentation for Ambient Ionization and Miniature Mass Spectrometers","authors":"Barry L. Smith, Thomas Hankinson, Simon Maher","doi":"10.1146/annurev-anchem-061522-040824","DOIUrl":"https://doi.org/10.1146/annurev-anchem-061522-040824","url":null,"abstract":"We critically evaluate the current status of portable mass spectrometry (pMS), particularly where this aligns with ambient ionization. Assessing the field of pMS can be quite subjective, especially in relation to the portable aspects of design, deployment, and operation. In this review, we discuss what it means to be portable and introduce a set of criteria by which pMS and ambient ionization sources can be assessed. Moreover, we consider the recent literature in terms of the most popular and significant advances in portable instrumentation for ambient ionization and miniature mass spectrometers. Finally, emerging trends and exciting future prospects are discussed and some recommendations are offered.","PeriodicalId":50758,"journal":{"name":"Annual Review of Analytical Chemistry","volume":"95 1","pages":""},"PeriodicalIF":8.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140628534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-19DOI: 10.1146/annurev-anchem-061622-034229
Tianyu Jiang, Xiaoyu Bai, Minyong Li
Bioluminescence imaging (BLI) is a powerful method for visualizing biological processes and tracking cells. Engineered bioluminescent bacteria that utilize luciferase-catalyzed biochemical reactions to generate luminescence have become useful analytical tools for in vitro and in vivo bacterial imaging. Accordingly, this review initially introduces the development of engineered bioluminescent bacteria that use different luciferase–luciferin pairs as analytical tools and their applications for in vivo BLI, including real-time bacterial tracking of infection, probiotic investigation, tumor-targeted therapy, and drug screening. Applications of engineered bioluminescent bacteria as whole-cell biosensors for sensing biological changes in vitro and in vivo are then discussed. Finally, we review the optimizations and future directions of bioluminescent bacteria for imaging. This review aims to provide fundamental insights into bacterial BLI and highlight the potential development of this technique in the future.
生物发光成像（BLI）是一种可视化生物过程和追踪细胞的强大方法。利用荧光素酶催化的生化反应产生发光的工程生物发光细菌已成为体外和体内细菌成像的有用分析工具。因此，本综述初步介绍了使用不同荧光素酶-荧光素对作为分析工具的工程生物发光细菌的发展及其在体内 BLI 中的应用，包括实时细菌感染跟踪、益生菌研究、肿瘤靶向治疗和药物筛选。然后讨论了工程生物发光细菌作为全细胞生物传感器在体外和体内感知生物变化的应用。最后，我们回顾了用于成像的生物发光细菌的优化和未来发展方向。本综述旨在提供有关细菌 BLI 的基本见解，并强调该技术在未来的潜在发展。
{"title":"Advances in the Development of Bacterial Bioluminescence Imaging","authors":"Tianyu Jiang, Xiaoyu Bai, Minyong Li","doi":"10.1146/annurev-anchem-061622-034229","DOIUrl":"https://doi.org/10.1146/annurev-anchem-061622-034229","url":null,"abstract":"Bioluminescence imaging (BLI) is a powerful method for visualizing biological processes and tracking cells. Engineered bioluminescent bacteria that utilize luciferase-catalyzed biochemical reactions to generate luminescence have become useful analytical tools for in vitro and in vivo bacterial imaging. Accordingly, this review initially introduces the development of engineered bioluminescent bacteria that use different luciferase–luciferin pairs as analytical tools and their applications for in vivo BLI, including real-time bacterial tracking of infection, probiotic investigation, tumor-targeted therapy, and drug screening. Applications of engineered bioluminescent bacteria as whole-cell biosensors for sensing biological changes in vitro and in vivo are then discussed. Finally, we review the optimizations and future directions of bioluminescent bacteria for imaging. This review aims to provide fundamental insights into bacterial BLI and highlight the potential development of this technique in the future.","PeriodicalId":50758,"journal":{"name":"Annual Review of Analytical Chemistry","volume":"40 1","pages":""},"PeriodicalIF":8.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140628531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-12DOI: 10.1146/annurev-anchem-061622-030223
Tanner W. Young, Michael P. Kappler, Ethan D. Call, Quintin J. Brown, Stephen C. Jacobson
Single-entity (or digital) measurements enhance sensitivity (10- to 100-fold improvement) and uncover heterogeneity within a population (one event in 100 to 10,000). Many biological systems are significantly influenced by rare or infrequent events, and determining what species is present, in what quantity, and role of that species is critically important to unraveling many questions. To develop these measurement systems, resistive-pulse sensing is used as a label-free, single-particle detection technique and can be combined with a range of functional elements, e.g., mixers, reactors, filters, separators, and pores. Virtually, any two-dimensional layout of the micro- and nanofluidic conduits can be envisioned, designed, and fabricated in the plane of the device. Multiple nanopores in series lead to higher-precision measurements of particle size, shape, and charge, and reactions coupled directly with the particle-size measurements improve temporal response. Moreover, other detection techniques, e.g., fluorescence, are highly compatible with the in-plane format. These integrated in-plane nanofluidic devices expand the toolbox of what is possible with single-entity measurements.
{"title":"Integrated In-Plane Nanofluidic Devices for Resistive-Pulse Sensing","authors":"Tanner W. Young, Michael P. Kappler, Ethan D. Call, Quintin J. Brown, Stephen C. Jacobson","doi":"10.1146/annurev-anchem-061622-030223","DOIUrl":"https://doi.org/10.1146/annurev-anchem-061622-030223","url":null,"abstract":"Single-entity (or digital) measurements enhance sensitivity (10- to 100-fold improvement) and uncover heterogeneity within a population (one event in 100 to 10,000). Many biological systems are significantly influenced by rare or infrequent events, and determining what species is present, in what quantity, and role of that species is critically important to unraveling many questions. To develop these measurement systems, resistive-pulse sensing is used as a label-free, single-particle detection technique and can be combined with a range of functional elements, e.g., mixers, reactors, filters, separators, and pores. Virtually, any two-dimensional layout of the micro- and nanofluidic conduits can be envisioned, designed, and fabricated in the plane of the device. Multiple nanopores in series lead to higher-precision measurements of particle size, shape, and charge, and reactions coupled directly with the particle-size measurements improve temporal response. Moreover, other detection techniques, e.g., fluorescence, are highly compatible with the in-plane format. These integrated in-plane nanofluidic devices expand the toolbox of what is possible with single-entity measurements.","PeriodicalId":50758,"journal":{"name":"Annual Review of Analytical Chemistry","volume":"43 1","pages":""},"PeriodicalIF":8.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140566201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-11DOI: 10.1146/annurev-anchem-061422-020428
Wei-Wei Wang, Hao Yan, Yu Gu, Jiawei Yan, Bing-Wei Mao
The electrochemical interface formed between an electrode and an electrolyte significantly affects the rate and mechanism of the electrode reaction through its structure and properties, which vary across the interface. The scope of the interface has been expanded, along with the development of energy electrochemistry, where a solid-electrolyte interphase may form on the electrode and the active materials change properties near the surface region. Developing a comprehensive understanding of electrochemical interfaces and interphases necessitates three-dimensional spatial resolution characterization. Atomic force microscopy (AFM) offers advantages of imaging and long-range force measurements. Here we assess the capabilities of AFM by comparing the force curves of different regimes and various imaging modes for in situ characterizing of electrochemical interfaces and interphases. Selected examples of progress on work related to the structures and processes of electrode surfaces, electrical double layers, and lithium battery systems are subsequently illustrated. Finally, this review provides perspectives on the future development of electrochemical AFM.
{"title":"In Situ Electrochemical Atomic Force Microscopy: From Interfaces to Interphases","authors":"Wei-Wei Wang, Hao Yan, Yu Gu, Jiawei Yan, Bing-Wei Mao","doi":"10.1146/annurev-anchem-061422-020428","DOIUrl":"https://doi.org/10.1146/annurev-anchem-061422-020428","url":null,"abstract":"The electrochemical interface formed between an electrode and an electrolyte significantly affects the rate and mechanism of the electrode reaction through its structure and properties, which vary across the interface. The scope of the interface has been expanded, along with the development of energy electrochemistry, where a solid-electrolyte interphase may form on the electrode and the active materials change properties near the surface region. Developing a comprehensive understanding of electrochemical interfaces and interphases necessitates three-dimensional spatial resolution characterization. Atomic force microscopy (AFM) offers advantages of imaging and long-range force measurements. Here we assess the capabilities of AFM by comparing the force curves of different regimes and various imaging modes for in situ characterizing of electrochemical interfaces and interphases. Selected examples of progress on work related to the structures and processes of electrode surfaces, electrical double layers, and lithium battery systems are subsequently illustrated. Finally, this review provides perspectives on the future development of electrochemical AFM.","PeriodicalId":50758,"journal":{"name":"Annual Review of Analytical Chemistry","volume":"1 1","pages":""},"PeriodicalIF":8.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140566094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-10DOI: 10.1146/annurev-anchem-061622-023326
Alba Espargaró, Irene Álvarez-Berbel, Maria Antònia Busquets, Raimon Sabate
Amyloid-related diseases, such as Alzheimer's and Parkinson's disease, are devastating conditions caused by the accumulation of abnormal protein aggregates known as amyloid fibrils. While assays involving animal models are essential for understanding the pathogenesis and developing therapies, a wide array of standard analytical techniques exists to enhance our understanding of these disorders. These techniques provide valuable information on the formation and propagation of amyloid fibrils, as well as the pharmacokinetics and pharmacodynamics of candidate drugs. Despite ethical concerns surrounding animal use, animal models remain vital tools in the search for treatments. Regardless of the specific animal model chosen, the analytical methods used are usually standardized. Therefore, the main objective of this review is to categorize and outline the primary analytical methods used in in vivo assays for amyloid-related diseases, highlighting their critical role in furthering our understanding of these disorders and developing effective therapies.
{"title":"In Vivo Assays for Amyloid-Related Diseases","authors":"Alba Espargaró, Irene Álvarez-Berbel, Maria Antònia Busquets, Raimon Sabate","doi":"10.1146/annurev-anchem-061622-023326","DOIUrl":"https://doi.org/10.1146/annurev-anchem-061622-023326","url":null,"abstract":"Amyloid-related diseases, such as Alzheimer's and Parkinson's disease, are devastating conditions caused by the accumulation of abnormal protein aggregates known as amyloid fibrils. While assays involving animal models are essential for understanding the pathogenesis and developing therapies, a wide array of standard analytical techniques exists to enhance our understanding of these disorders. These techniques provide valuable information on the formation and propagation of amyloid fibrils, as well as the pharmacokinetics and pharmacodynamics of candidate drugs. Despite ethical concerns surrounding animal use, animal models remain vital tools in the search for treatments. Regardless of the specific animal model chosen, the analytical methods used are usually standardized. Therefore, the main objective of this review is to categorize and outline the primary analytical methods used in in vivo assays for amyloid-related diseases, highlighting their critical role in furthering our understanding of these disorders and developing effective therapies.","PeriodicalId":50758,"journal":{"name":"Annual Review of Analytical Chemistry","volume":"119 1","pages":""},"PeriodicalIF":8.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140566112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-10DOI: 10.1146/annurev-anchem-083023-024546
Madeline E. Colley, Allison B. Esselman, Claire F. Scott, Jeffrey M. Spraggins
Imaging mass spectrometry (IMS) enables highly multiplexed, untargeted tissue mapping for a broad range of molecular classes, facilitating in situ biological discovery. Yet, challenges persist in molecular specificity, which is the ability to discern one molecule from another, and spatial specificity, which is the ability to link untargeted imaging data to specific tissue features. Instrumental developments have dramatically improved IMS spatial resolution, allowing molecular observations to be more readily associated with distinct tissue features across spatial scales, ranging from larger anatomical regions to single cells. High-performance mass analyzers and systems integrating ion mobility technologies are also becoming more prevalent, further improving molecular coverage and the ability to discern chemical identity. This review provides an overview of recent advancements in high-specificity IMS that are providing critical biological context to untargeted molecular imaging, enabling integrated analyses, and addressing advanced biomedical research applications.
{"title":"High-Specificity Imaging Mass Spectrometry","authors":"Madeline E. Colley, Allison B. Esselman, Claire F. Scott, Jeffrey M. Spraggins","doi":"10.1146/annurev-anchem-083023-024546","DOIUrl":"https://doi.org/10.1146/annurev-anchem-083023-024546","url":null,"abstract":"Imaging mass spectrometry (IMS) enables highly multiplexed, untargeted tissue mapping for a broad range of molecular classes, facilitating in situ biological discovery. Yet, challenges persist in molecular specificity, which is the ability to discern one molecule from another, and spatial specificity, which is the ability to link untargeted imaging data to specific tissue features. Instrumental developments have dramatically improved IMS spatial resolution, allowing molecular observations to be more readily associated with distinct tissue features across spatial scales, ranging from larger anatomical regions to single cells. High-performance mass analyzers and systems integrating ion mobility technologies are also becoming more prevalent, further improving molecular coverage and the ability to discern chemical identity. This review provides an overview of recent advancements in high-specificity IMS that are providing critical biological context to untargeted molecular imaging, enabling integrated analyses, and addressing advanced biomedical research applications.","PeriodicalId":50758,"journal":{"name":"Annual Review of Analytical Chemistry","volume":"26 1","pages":""},"PeriodicalIF":8.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140566216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-10DOI: 10.1146/annurev-anchem-061622-030919
Kathryn J. Vannoy, Myles Quinn Edwards, Christophe Renault, Jeffrey E. Dick
Analytical techniques operating at the nanoscale introduce confinement as a tool at our disposal. This review delves into the phenomenon of accelerated reactivity within micro- and nanodroplets. A decade of accelerated reactivity observations was succeeded by several years of fundamental studies aimed at mechanistic enlightenment. Herein, we provide a brief historical context for rate enhancement in micro- and nanodroplets and summarize the mechanisms that have been proposed to contribute to such extraordinary reactivity. We highlight recent electrochemical reports that make use of restricted mass transfer to enhance electrochemical reactions and/or quantitatively measure reaction rates within droplet-confined electrochemical cells. A comprehensive approach to nanodroplet reactivity is paramount to understanding how nature takes advantage of these systems to provide life on Earth and, in turn, how to harness the full potential of such systems.
{"title":"An Electrochemical Perspective on Reaction Acceleration in Droplets","authors":"Kathryn J. Vannoy, Myles Quinn Edwards, Christophe Renault, Jeffrey E. Dick","doi":"10.1146/annurev-anchem-061622-030919","DOIUrl":"https://doi.org/10.1146/annurev-anchem-061622-030919","url":null,"abstract":"Analytical techniques operating at the nanoscale introduce confinement as a tool at our disposal. This review delves into the phenomenon of accelerated reactivity within micro- and nanodroplets. A decade of accelerated reactivity observations was succeeded by several years of fundamental studies aimed at mechanistic enlightenment. Herein, we provide a brief historical context for rate enhancement in micro- and nanodroplets and summarize the mechanisms that have been proposed to contribute to such extraordinary reactivity. We highlight recent electrochemical reports that make use of restricted mass transfer to enhance electrochemical reactions and/or quantitatively measure reaction rates within droplet-confined electrochemical cells. A comprehensive approach to nanodroplet reactivity is paramount to understanding how nature takes advantage of these systems to provide life on Earth and, in turn, how to harness the full potential of such systems.","PeriodicalId":50758,"journal":{"name":"Annual Review of Analytical Chemistry","volume":"10 1","pages":""},"PeriodicalIF":8.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140566215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-10DOI: 10.1146/annurev-anchem-061622-125954
Cassandra Herbert, Satenik Valesyan, Jennifer Kist, Patrick A. Limbach
Ribonucleic acids (RNAs) are key biomolecules responsible for the transmission of genetic information, the synthesis of proteins, and modulation of many biochemical processes. They are also often the key components of viruses. Synthetic RNAs or oligoribonucleotides are becoming more widely used as therapeutics. In many cases, RNAs will be chemically modified, either naturally via enzymatic systems within a cell or intentionally during their synthesis. Analytical methods to detect, sequence, identify, and quantify RNA and its modifications have demands that far exceed requirements found in the DNA realm. Two complementary platforms have demonstrated their value and utility for the characterization of RNA and its modifications: mass spectrometry and next-generation sequencing. This review highlights recent advances in both platforms, examines their relative strengths and weaknesses, and explores some alternative approaches that lie at the horizon.
核糖核酸（RNA）是负责传递遗传信息、合成蛋白质和调节许多生化过程的关键生物大分子。它们通常也是病毒的关键成分。合成 RNA 或寡核苷酸正越来越广泛地用作治疗药物。在许多情况下，RNA 会通过细胞内的酶系统自然或在合成过程中有意进行化学修饰。检测、测序、识别和量化 RNA 及其修饰的分析方法的要求远远超过 DNA 领域的要求。质谱法和新一代测序法这两种互补平台已经证明了它们在表征 RNA 及其修饰方面的价值和实用性。这篇综述重点介绍了这两种平台的最新进展，研究了它们的相对优缺点，并探讨了一些即将出现的替代方法。
{"title":"Analysis of RNA and Its Modifications","authors":"Cassandra Herbert, Satenik Valesyan, Jennifer Kist, Patrick A. Limbach","doi":"10.1146/annurev-anchem-061622-125954","DOIUrl":"https://doi.org/10.1146/annurev-anchem-061622-125954","url":null,"abstract":"Ribonucleic acids (RNAs) are key biomolecules responsible for the transmission of genetic information, the synthesis of proteins, and modulation of many biochemical processes. They are also often the key components of viruses. Synthetic RNAs or oligoribonucleotides are becoming more widely used as therapeutics. In many cases, RNAs will be chemically modified, either naturally via enzymatic systems within a cell or intentionally during their synthesis. Analytical methods to detect, sequence, identify, and quantify RNA and its modifications have demands that far exceed requirements found in the DNA realm. Two complementary platforms have demonstrated their value and utility for the characterization of RNA and its modifications: mass spectrometry and next-generation sequencing. This review highlights recent advances in both platforms, examines their relative strengths and weaknesses, and explores some alternative approaches that lie at the horizon.","PeriodicalId":50758,"journal":{"name":"Annual Review of Analytical Chemistry","volume":"91 1","pages":""},"PeriodicalIF":8.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140566202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：