ANTI-INFLAMMATORY AND ANTI-CANDIDAL ACTIVITY OF CORIANDRUM SATIVUM SEED EXTRACTS AGAINST CANDIDA ALBICANS AND CANDIDA TROPICALIS

Abstract

Background: Candidiasis in not uncommon among hospitalized patients. The fungal virulence and antifungal drug resistance make the host-parasite interactions complicated. The threat of azole drug resistance along with scarcity of antifungal therapies prompted the necessity of novel drug development from various natural sources. The objective of this study was to evaluate the anticandidal activity of Coriandrum sativum seed extracts against Candida albicans and Candida tropilcalis. Materials & Methods: This study was conducted at Department of Basic Medical Sciences, College of Medicine, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia from July 20, 2021 to September 26, 2021. The study analyzed anti-inflammatory assays via inhibition of albumin denaturation and hemolysis inhibition and anticandidal activity of the extract against Candida albicans and Candida tropilcalis by well diffusion method. The minimum inhibitory concentration (MIC) of the extract was also determined against both the fungi. Results: Anti-inflammatory activity of Coriandrum sativum methanolic seed extracts were evaluated at five different concentrations from 100 µg to 1000 µg. The highest concentration showed 55.31±0.77% of inhibition of albumin denaturation, whereas the aspirin, the standard used, showed 80.33±0.32%. Coriandrum sativum seed extracts at 250 mg/ml, 500 mg/ml and 1000 mg/ml produced zones of growth inhibition with diameters 12.66±0.57, 17.66±0.57 and 24.33±1 mm against Candida albicans, and 10.33±0.57, 16.66±0.57 and 21.66±0.57 mm against Candida tropicalis. The MICs of the Coriandrum sativum seed extracts against Candida albicans was 1.95 mg and Candida tropicalis was 3.9 mg. Conclusion: Phytochemical compounds in methanolic seed extracts of Coriandrum sativum showed significant anticandidal activity against Candida albicans and Candida tropicalis with highest zone of inhibition at 1000 µg extract concentration. The lower MICs of Coriandrum sativum seed extracts against Candida albicans and Candida tropicalis were also significant. Considering all these findings along with the antioxidant activities, it could be suggested that Coriandrum sativum seed extracts can further be investigated for development of novel anticandidal drugs.