EVALUATION OF STRESS OF ENVIRONMENTAL RELEVANT CONCENTRATION OF GLYPHOSATE PESTICIDE ON LAMELLIDENS MARGINALIS

Abstract

We evaluated glyphosate (GLP) toxicity on Lamellidens marginalis by using oxidative andgenotoxic stress markers. The study provides evidence of oxidative stress and altered activities ofantioxidative enzymes in bivalve (gill, foot, mantle, muscle, and hepatopancreas) upon exposureto an environmentally relevant concentration of glyphosate organophosphate pesticide (1 mg l-1).The GLP exposure periods were 7 (T1) and 14 (T2) days, followed by a recovery period of 4 days(R1 & R2) respectively. GLP exposure showed a positive correlation between oxidative stress andduration of exposure. A catalase induction trend was observed in both the treated groups.Induction or inhibition of Super oxide dismutase (SOD) enzyme activities were tissue-specificafter GLP exposure. The technical grade GLP has genotoxic potential, studied with the help ofcomet assay on gill tissues. Longer duration of exposure has significantly increased cometparameters such as tail length, tail DNA percentage as well as olive tail movement as compared tocontrol bivalve. However, bivalves recovered significantly after the four days of the recoveryperiod. The results suggested oxidative stress and genotoxic potential of GLP, evidenced by alteredactivities of antioxidative enzymes and induction of comet parameters. Even though, the animalsexperienced the stress of GLP exposure, however, recovery potential of bivalves was noteworthyafter the removal of the stress.