Cahyono Hadi, D. Dasuki, A. Sadewa, I. Astuti, Awal Prasetyo
{"title":"Nitric oxide (NO) level of the follicular fluid in endometriosis patients","authors":"Cahyono Hadi, D. Dasuki, A. Sadewa, I. Astuti, Awal Prasetyo","doi":"10.20473/MOG.V26I12018.29-35","DOIUrl":null,"url":null,"abstract":"Objectives: To ascertain the nitric oxide (NO) level of the follicular fluid of endometriosis sufferers compared with that of the non-endometriosis patients.Materials and Methods: Follicular fluid was taken from 64 women, but only 54 continued with further laboratory test. Women with endometriosis had their follicular fluid taken during laparoscopy, while the follicular fluid from non-endometriosis women was taken during tubectomy. Nitric Oxide level was analyzed using the PCR-RFLP method. Data were analyzed using software STATA Intercooled Version 11.Results: NO level in endometriosis is higher than that in non-endometriosis and has a significant relationship with all the variables (age, parity, dysmenorrhea, dyspareunia, types of endometriosis, and grade of endometriosis).Conclusion: The NO level in endometriosis is higher than that in non-endometriosis. The distribution of the polymorphism of allele GG is greater than that in non-endometriosis. The existence of polymorphism GG caused a higher eNOS, increasing NO level, causing a decrease in fertility which derived from the degenera-tive oocyte.","PeriodicalId":52893,"journal":{"name":"Majalah Obstetri dan Ginekologi","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2018-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Majalah Obstetri dan Ginekologi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20473/MOG.V26I12018.29-35","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objectives: To ascertain the nitric oxide (NO) level of the follicular fluid of endometriosis sufferers compared with that of the non-endometriosis patients.Materials and Methods: Follicular fluid was taken from 64 women, but only 54 continued with further laboratory test. Women with endometriosis had their follicular fluid taken during laparoscopy, while the follicular fluid from non-endometriosis women was taken during tubectomy. Nitric Oxide level was analyzed using the PCR-RFLP method. Data were analyzed using software STATA Intercooled Version 11.Results: NO level in endometriosis is higher than that in non-endometriosis and has a significant relationship with all the variables (age, parity, dysmenorrhea, dyspareunia, types of endometriosis, and grade of endometriosis).Conclusion: The NO level in endometriosis is higher than that in non-endometriosis. The distribution of the polymorphism of allele GG is greater than that in non-endometriosis. The existence of polymorphism GG caused a higher eNOS, increasing NO level, causing a decrease in fertility which derived from the degenera-tive oocyte.
目的:探讨子宫内膜异位症患者与非子宫内膜异位症患者卵泡液中一氧化氮(NO)水平的差异。材料和方法:从64名妇女中提取卵泡液,但只有54名继续进行进一步的实验室检查。子宫内膜异位症患者的卵泡液在腹腔镜下采集,而非子宫内膜异位症患者的卵泡液在输卵管切除术时采集。采用PCR-RFLP法分析一氧化氮水平。数据分析使用STATA intercooling Version 11软件。结果:子宫内膜异位症患者NO水平高于非子宫内膜异位症患者,且与年龄、胎次、痛经、性交困难、子宫内膜异位症类型、子宫内膜异位症分级等因素均有显著相关。结论:子宫内膜异位症患者一氧化氮水平高于非子宫内膜异位症患者。GG等位基因多态性分布大于非子宫内膜异位症。多态性GG的存在导致eNOS升高,NO水平升高,导致卵母细胞变性导致的生育能力下降。