J. J. Alba-Romero, P. Ruíz-Flores, G. Castro-Escarpulli, S. Hernández-González, A. Martínez-Romero, J. L. Ortega-Sánchez
{"title":"Analysis of Cuorum Sensing-Dependent Virulence Factors and Drug Resistance in Pseudomonas Aeruginosa Strains","authors":"J. J. Alba-Romero, P. Ruíz-Flores, G. Castro-Escarpulli, S. Hernández-González, A. Martínez-Romero, J. L. Ortega-Sánchez","doi":"10.47752/SJMH.41.46.51","DOIUrl":null,"url":null,"abstract":"The objective was to analyze the virulence factors dependent on Cuorum Sensing and drug resistance in strains of Pseudomonas aeruginosa. Virulence factors such as pyocyanin, beta-lactamase, biofilm, and antibiotic resistance were determined in 95 strains of P. aeruginosa isolated from hospitalized patients. Genus and species were identified by protein analysis by MALDI-TOF. 100% of the strains were resistant to at least one drug and the highest proportion was 32 strains resistant to 4 drugs and 5 resistant PAM strains. In the analysis of virulence factors, 98.8% produce at least one virulence factor and 48.9% are beta-lactamase producers. Therefore, it is concluded that P. aeruginosa strains isolated from clinical samples constitute a risk factor for hospitalized patients.","PeriodicalId":93143,"journal":{"name":"Sumerianz journal of medical and healthcare","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sumerianz journal of medical and healthcare","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47752/SJMH.41.46.51","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The objective was to analyze the virulence factors dependent on Cuorum Sensing and drug resistance in strains of Pseudomonas aeruginosa. Virulence factors such as pyocyanin, beta-lactamase, biofilm, and antibiotic resistance were determined in 95 strains of P. aeruginosa isolated from hospitalized patients. Genus and species were identified by protein analysis by MALDI-TOF. 100% of the strains were resistant to at least one drug and the highest proportion was 32 strains resistant to 4 drugs and 5 resistant PAM strains. In the analysis of virulence factors, 98.8% produce at least one virulence factor and 48.9% are beta-lactamase producers. Therefore, it is concluded that P. aeruginosa strains isolated from clinical samples constitute a risk factor for hospitalized patients.