Ficophagus annulatae n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus annulata in China

Abstract

A new species of the genus Ficophagus was recovered from the syconia of Ficus annulata from Xishuangbanna, Yunnan province, China. It is described herein as Ficophagus annulatae n. sp. and is characterised by possessing the combined characters of a short PUS (9-18 μm or 0.3-0.6 VBD long), an excretory pore (EP) located at the level between stylet basal knobs and metacorpus, presence of crustaformeria, amoeboid sperm, three pairs of subventral papillae on the male tail (one pair just adcloacal (P2), one pair halfway between cloacal aperture and tail terminus (P3), and one pair near tail tip (P4)), rounded male tail tip without mucron, absence of gubernaculum and bow-shaped spicule with indistinct rostrum. Ficophagus annulatae n. sp. was differentiated from other sequenced species by the partial small subunit (SSU) rRNA gene and D2-D3 expansion segments of the large subunit (LSU) rRNA gene. Phylogenetic analysis with the LSU D2-D3 expansion segment sequence suggested that F. annulatae n. sp. is clustered in the same highly supported monophyletic clade with F. benjamina, F. curtipes and F. microcarpus. It differs morphologically from these species in EP position, spicule and uterus morphology, and some morphometric characters.