Molecular detection of caspase 3, 8, 9 genes and ADIPOR1 (rs2275738) polymorphism in colorectal cancer

Abstract

The main type of cancer-related world casualties is colorectal cancer (CRC). Colorectal cancer occurs due to many important factors, including genes. These genes are programmed death genes such as caspase 3, 8, 9 and variants in receptor of adiponectin 1 (ADIPOR1) that are related to ‘CRC’ risk. A study was conducted on the high and low gene expression of caspase 3, 8, and 9 genes and their association with the risk of rectal cancer, as well as the association of genotypes in adiponectin receptor 1 (ADIPOR1) to indicate the extent to which these genotypes are related to colorectal cancer compared with the control group. This study was conducted from 2021 to 2022 on 100 patients with colorectal cancer after being medically diagnosed and compared them with 100 healthy individuals. Overall mRNA for all blood samples were taken out and cDNA was produced, use of SYBR Green mix of PCR master and actual gene expression method, quantifying the transcript level of target genes. The polymorphism of the genotypes of ADIPOR1 (rs2275738) was studied using the PCR–RFLP method. Experiments revealed the mRNA expressions of caspases 9 and 3 in patients with lower CRC compared to healthy control patients and controls. Nonetheless, ADIPOR1 (rs2275738) “CC + CT” genotype in comparison to the “TT” genotype was more repeatedly found in CRC cases than in controls. Smoking, body mass index, and family history of colorectal cancer were higher in the CRC group when compared with the control group.