Identification and in silico structural and functional analysis of a histone H2A-derived antimicrobial peptide from teleost fishes

Q4 Biochemistry, Genetics and Molecular Biology Animal Gene Pub Date : 2023-06-01 DOI:10.1016/j.angen.2023.200148

K.L. Dhanya Lenin, Athira Raveendran, Swapna P. Antony

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引用次数: 3

Abstract

Background

Antimicrobial peptides (AMPs) are small peptides that form the first line of defence in all living organisms. In addition to conventional AMPs, there exist molecules that are often not regarded as part of the innate defences, but possess antimicrobial activity. Histone H2A-derived peptides are a group of unconventional AMPs that represent very ancient defence factors, re-used in new ways during evolution. The present study attempts to compare histone H2A-derived AMPs from four commercially important teleost fishes inhabiting various habitats, viz. Asian sea bass (Lates calcarifer), tank goby (Glossogobius giuris), grey mullet (Mugil cephalus), and Malabar trevally (Carangoides malabaricus).

Methods and results

RT-PCR amplification of the mRNAs extracted from these fishes yielded H2A-derived AMP genes with slight differences in the nucleotide sequences, nevertheless with similar translated amino acid sequences. The amino acid sequence was found to possess marked similarity at the N-terminal region and showed antimicrobial motif sequence characteristic of histone H2A-derived AMPs. Phylogenetic analysis showed an evolutionary relationship with invertebrate ones suggesting a common ancestral origin for the vertebrate and invertebrate histone H2A. The secondary structure of histone H2A-derived AMP showed the presence of α-helices and intermittent coils having a proline hinge connecting the N- and C-terminus. The peptide was also predicted to be a cell-penetrating one.

Conclusion

In silico structural and functional characterization of histone H2A-derived AMP revealed its potential antibacterial property and point towards the possibility of being an efficient AMP with the potential of being made use of in therapeutics.

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硬骨鱼组蛋白H2A抗菌肽的鉴定及结构功能分析

背景抗菌肽（AMPs）是在所有生物体中形成第一道防线的小肽。除了传统的AMPs，还有一些分子通常不被视为先天防御的一部分，但具有抗菌活性。组蛋白H2A衍生肽是一组非常规的AMP，代表了非常古老的防御因子，在进化过程中以新的方式重新使用。本研究试图比较居住在不同栖息地的四种商业上重要的硬骨鱼的组蛋白H2A衍生的AMPs，即亚洲鲈鱼（Lates calcarifer）、槽虾虎鱼（Glossogobius giuris）、灰乌鱼（Mugil cephalus），和Malabar trevally（Carangoides malabaricus）。方法和结果对从这些鱼类中提取的mRNA进行RT-PCR扩增，产生了H2A衍生的AMP基因，核苷酸序列略有差异，但翻译的氨基酸序列相似。发现该氨基酸序列在N-末端区域具有显著的相似性，并显示出组蛋白H2A衍生的AMPs的抗菌基序序列特征。系统发育分析显示与无脊椎动物存在进化关系，这表明脊椎动物和无脊椎动物组蛋白H2A有共同的祖先起源。组蛋白H2A衍生的AMP的二级结构显示存在α-螺旋和具有连接N-和C-末端的脯氨酸铰链的间断螺旋。该肽也被预测为穿透细胞的肽。结论组蛋白H2A衍生AMP的计算机结构和功能表征揭示了其潜在的抗菌特性，并指出其可能成为一种具有治疗潜力的高效AMP。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Animal Gene Agricultural and Biological Sciences-Insect Science

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期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.