Hypocotyl elongation based on HY5 transcription factor in cold resistant winter rapeseed (Brassica napus L.)

Q3 Agricultural and Biological Sciences Oil Crop Science Pub Date : 2022-03-01 DOI:10.1016/j.ocsci.2022.02.005

Jiaojiao Jin , Wancang Sun , Junyan Wu , Yan Fang , Xuecai Li , Li Ma , Lijun Liu , Rui Zeng

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引用次数: 1

Abstract

Winter rape (Brassica napus L.) is better than other edible oil crops in China, but poor cold resistance is the key factor restricting its development. Hypocotyl length was found closely related to cold tolerance. The correlation between hypocotyl length and semi-lethal low temperature was significant, and the highest correlation between hypocotyl length and LT50 of autumn sowing was 0.9557. When the hypocotyl were treated at low temperature, the cells were seriously damaged and formed cavity structures, with cell walls seriously damaged or merged into each other. The positive regulation gene of hypocotyl length in resistant line of VHTSG 10 was identified as HY5 (transcription factor HY5-like) by qPCR, and bZIP transcription factor was found to be its conserved domain. Fused gene by GFP and HY5 from VHTSG 10 was transient transferred into Nicotiana benthamiana cells. Corresponding to the 35S:GFP widely distributed in plasma membrane of leaf epidermis, the fusion protein 35S:HY5-GFP was mainly distributed in nucleus. Thus we regarded BnHY5 gene is a key gene related to cold tolerance and hypocotyl length in B. napus.

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基于HY5转录因子的耐寒油菜下胚轴伸长研究

油菜(Brassica napus L.)是中国较好的油料作物，但抗寒性差是制约其发展的关键因素。下胚轴长度与耐寒性密切相关。下胚轴长度与半致死低温相关显著，秋播时下胚轴长度与LT50相关性最高，为0.9557。低温处理下胚轴时，细胞严重受损，形成空洞结构，细胞壁严重受损或相互合并。通过qPCR鉴定抗性品系下胚轴长度的正调控基因为HY5(转录因子HY5样)，bZIP转录因子为其保守结构域。将VHTSG - 10中GFP与HY5融合的基因瞬间转移到烟叶细胞中。与35S:GFP广泛分布于叶表皮质膜相对应，融合蛋白35S:HY5-GFP主要分布于细胞核内。因此，我们认为BnHY5基因是甘蓝型油菜耐寒性和下胚轴长度相关的关键基因。

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