Molecular detection of Anaplasma marginale in stable flies Stomoxys calcitrans (Diptera: Muscidae) feeding on a tick-free bovine herd

IF 0.2 4区 农林科学 Q2 Veterinary Veterinaria Mexico Pub Date : 2018-01-26 DOI:10.21753/vmoa.5.1.436
C. R. B. Garfias, T. Rodríguez, C. Rojas, J. Lira, J. A. Álvarez, Diego Polanco
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引用次数: 8

Abstract

Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Bautista-Garfias CR, Rodriguez T, Rojas C, Lira JJ, Alvarez JA, Polanco D. Molecular detection of Anaplasma marginale in stable flies Stomoxys calcitrans (Diptera: Muscidae) feeding on a tick-free bovine herd. Veterinaria Mexico OA. 2018;5(1). doi: 10.21753/vmoa.5.1.436 . Data on the implication of the stable fly ( Stomoxys calcitrans , Diptera: Mus­cidae), in the transmission of Anaplasma marginale in tick-free cattle is scarce. Hence, the objective of this investigation was to detect the presence of DNA from A. marginale in stable flies caught near a bovine herd, which has been maintained free of ticks for 40 years, and occasionally presents some clinical cases of anaplasmosis. Twenty-four batches of S. calcitrans (15 flies each) were collected in the morning and in the afternoon twice a week (except for one catch in one week of September and one catch in one week of December) during 12 catching days in a period of four months. Their DNA was obtained and analysed by nested PCR (nPCR) to identify the presence of A. marginale DNA. Seven of the batches (29.16 %) were pos­itive for A. marginale , as detected by an nPCR that targets the A. marginale msp5 gene. Similarly, in two out of 12 catching days (16.66 %), those S. calcitrans batches collected in the morning and in the afternoon, were pos­itive for A. marginale , while those S. calcitrans groups collected in three out of 12 catching days (25 %), were positive for the rickettsia. The obtained re­sults suggest that A. marginale transmission is carried out mechanically by S. calcitrans , favouring the circulation and maintenance of the microorganism in this particular bovine herd. Figure 1. A. Example of a stable fly Stomoxys calcitrans caught in this study (photograph: Carlos R. Bautista).
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以无蜱牛群为食的稳定蝇——钙口蝇(直翅目:蝇科)边缘无浆体的分子检测
墨西哥兽医OA ISSN:2448-6760引用如下:Bautista Garfias CR,Rodriguez T,Rojas C,Lira JJ,Alvarez JA,Polanco D.在以无蜱牛群为食的稳定蝇Stomoxys califrans(直翅目:蝇科)中边缘无浆体的分子检测。兽医墨西哥OA。2018年;5(1)。doi:10.21753/vmoa.5.1.436.关于稳定蝇(Stomoxys calcitrans,Diptera:Mus­cidae)在无蜱牛中传播边缘无浆体的影响的数据很少。因此,本研究的目的是检测在牛群附近捕获的稳定苍蝇中是否存在边缘A.marginale的DNA,牛群40年来一直没有蜱虫,偶尔也会出现一些无浆体病的临床病例。在四个月的12个捕获日内,每周两次(9月一周捕获一次,12月一周捕捉一次除外)在上午和下午采集24批钙化链球菌(每只15只)。获得它们的DNA并通过套式PCR(nPCR)进行分析,以鉴定边缘A.marginale DNA的存在。通过靶向A.marginale msp5基因的nPCR检测,其中7批(29.16%)对A.marginal是阳性的。同样,在12个捕获日中的2个(16.66%),上午和下午收集的S.calcitrans批次对边缘A.marginal呈阳性,而在12个捕捉日中的3个(25%)收集的S.cacitrans组对立克次体呈阳性。所获得的结果表明,A.marginale的传播是由S.calcitrans机械进行的,有利于微生物在这个特定牛群中的循环和维持。图1。A.在本研究中捕获的稳定苍蝇Stomoxys calcitrans的例子(照片:Carlos R.Bautista)。
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来源期刊
Veterinaria Mexico
Veterinaria Mexico VETERINARY SCIENCES-
自引率
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>12 weeks
期刊介绍: Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges. This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).
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