Extraction, purification and antioxidant activity of Juglans regia shell polysaccharide

Abstract

In recent years, the biological activity of plant polysaccharides has attracted more and more attention. Juglans regia was one of the four-dry fruits in the world, it has very high value in nutrition and health care value was very high. To better develop the nutritional and health effects of the J. regia shell polysaccharide, J. regia shell was used as raw material to prepare polysaccharide by the water extraction and alcohol precipitation method. The molecular weight, monosaccharide components, molecular structure characteristics and antioxidant activities of its derivatives were studied. Then, a series of meaningful data information for the study of the J. regia shell polysaccharide and its derivatives were obtained. The molecular weight of the polysaccharide was 9.45 × 10⁴ Da after the separation and purification by DEAE-52 and Sephadex G-100. The polysaccharides contained rhamnose, arabinose, galactose, glucose, xylose and galacturonic acid, and the molar ratio of each monosaccharide component was 6.7%: 16.5%: 28.3%: 11.2%: 12.5%: 24.8%. The molecular structure of J. regia shell polysaccharide contains β-glucoside bond, its structure may be → 4)-α-D-Galp-(1 → 4)-β-D-GalAp-(1 → 4)-α-D-Xylp-(1 → glycosidic bond). The study of antioxidant activity, J. regia shell polysaccharide and its derivatives showed good biological activities, especially the phosphorylated J. regia shell polysaccharide. The study of J. regia shell polysaccharide has important practical significance for improving the quality and efficiency of the J. regia industry chain.

Graphical Abstract