胶质芽孢杆菌( Bacillus mucilaginosus )在餐厨垃圾废水中生长条件优化

Q2 Environmental Science 环境科学研究 Pub Date : 2017-03-25 DOI:10.13198/J.ISSN.1001-6929.2017.01.50

郭新愿, 王攀, 任连海, 贺艳坤

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Abstract

为了探讨餐厨垃圾废水用作发酵基质生产液态解钾菌肥的可行性，选用胶质芽孢杆菌（ Bacillus mucilaginosus ）作为试验菌种，采用正交和单因素方法对相关生长因素进行了优化.结果表明，在餐厨垃圾废水中培养胶质芽孢杆菌经过3 d的调整期后进入对数生长期，6~7 d时活菌数达到最大，Ⅰ类废水活菌数为1.55×10 10 CFU/mL，Ⅱ类废水活菌数为6.60×10 10 CFU/mL.以Ⅱ类废水为基质进行正交试验确定的较优培养条件为pH=7、温度30℃、摇床转速160 r/min、接种量2.0%（V/V）.废水的pH和盐分对胶质芽孢杆菌的生长代谢影响极为显著：最适初始pH为7（活菌数为3.80×10 10 CFU/mL和9.20×10 10 CFU/mL）；随着 ρ （NaCl）的增加，活菌数先升高后快速降低，最适 ρ （NaCl）为4 g/L.Ⅰ类和Ⅱ类废水的最佳接种量分别为1.5%（活菌数为1.60×10 10 CFU/mL）和2.0%（活菌数为6.40×10 10 CFU/mL）.研究显示，胶质芽孢杆菌在餐厨垃圾废水中经过培养后可达到GB 20287-2006《农用微生物菌剂》中液态菌肥的活菌数（2.0×10 8 CFU/mL），经湿热处理后的Ⅱ类废水对胶质芽孢杆菌的生长有明显的促进作用.

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胶质芽孢杆菌( Bacillus mucilaginosus )在餐厨垃圾废水中生长条件优化

In order to explore the feasibility of using kitchen waste wastewater as a fermentation substrate to produce liquid potassium solubilizing bacterial fertilizer, Bacillus mucilaginosus was selected as the experimental strain, and the relevant growth factors were optimized using orthogonal and single factor methods. The results showed that after a 3-day adjustment period, Bacillus mucilaginosus entered a logarithmic growth phase when cultured in kitchen waste water, and the maximum viable bacterial count was reached at 6-7 days, The number of viable bacteria in Class I wastewater is 1.55 × 10 10 CFU/mL, Class II wastewater with a viable bacterial count of 6.60 × 10 10 CFU/mL. The optimal cultivation conditions determined by orthogonal experiments using Class II wastewater as the substrate were pH=7, temperature 30 ℃, shaking speed 160 r/min, and inoculation amount 2.0% (V/V). The pH and salt content of the wastewater had a significant impact on the growth and metabolism of Bacillus cereus: the optimal initial pH was 7 (with a viable bacterial count of 3.80) × 10 10 CFU/mL and 9.20 × 10 10 CFU/mL); With ρ The increase of (NaCl) leads to a rapid decrease in the number of viable bacteria, which is most suitable ρ The optimal inoculation amount for Class I and Class II wastewater with a NaCl concentration of 4 g/L. is 1.5% (with a viable bacterial count of 1.60), respectively × 10 10 CFU/mL) and 2.0% (6.40 viable bacteria) × 10 10 CFU/mL). Studies have shown that after cultivation in kitchen waste wastewater, Bacillus gelatinosus can reach the live bacterial count (2.0) of liquid microbial fertilizer in GB 20287-2006 "Agricultural Microbial Agents" × 10 8 CFU/mL), Class II wastewater treated with damp heat has a significant promoting effect on the growth of Bacillus cereus

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