Development of a specific monoclonal antibody-based icELISA for detection of arecoline in traditional Chinese medicines and fresh areca nuts

IF 1.7 3区 农林科学 Q3 CHEMISTRY, APPLIED Food and Agricultural Immunology Pub Date : 2022-02-04 DOI:10.1080/09540105.2021.2025347
Yunhe Wang, Mengying Ding, Hudong Ma, Jiao Wu, Hongwei Zhao, Yinglang Wan
{"title":"Development of a specific monoclonal antibody-based icELISA for detection of arecoline in traditional Chinese medicines and fresh areca nuts","authors":"Yunhe Wang, Mengying Ding, Hudong Ma, Jiao Wu, Hongwei Zhao, Yinglang Wan","doi":"10.1080/09540105.2021.2025347","DOIUrl":null,"url":null,"abstract":"ABSTRACT Arecoline, the dominant alkaloid existing in areca nuts, is an addictive substance and classified as a Group 2B potential human carcinogen. Currently, the detection of arecoline is mostly dependent on chromatography-based approaches, which are time-consuming and expensive. We used arecaidine as a hapten to produce a highly specific monoclonal antibody (mAb) against arecoline. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed using the mAb-A5H12, to detect arecoline in traditional Chinese medicines and fresh areca nuts. The icELISA indicated that the half maximum inhibition concentration (IC50) for arecoline was 67.9 ng/mL, with a working range of 10.1–502.6 ng/mL and a limit of detection (LOD) of 3.6 ng/mL. High-performance liquid chromatographic (HPLC) confirmed the accuracy and the working range of icELISA, suggesting that the icELISA approach based on the arecoline specific antibody could be a widely applicable and easy operation method in detection of arecoline in foods and medicines.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"113 - 126"},"PeriodicalIF":1.7000,"publicationDate":"2022-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food and Agricultural Immunology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/09540105.2021.2025347","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 4

Abstract

ABSTRACT Arecoline, the dominant alkaloid existing in areca nuts, is an addictive substance and classified as a Group 2B potential human carcinogen. Currently, the detection of arecoline is mostly dependent on chromatography-based approaches, which are time-consuming and expensive. We used arecaidine as a hapten to produce a highly specific monoclonal antibody (mAb) against arecoline. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed using the mAb-A5H12, to detect arecoline in traditional Chinese medicines and fresh areca nuts. The icELISA indicated that the half maximum inhibition concentration (IC50) for arecoline was 67.9 ng/mL, with a working range of 10.1–502.6 ng/mL and a limit of detection (LOD) of 3.6 ng/mL. High-performance liquid chromatographic (HPLC) confirmed the accuracy and the working range of icELISA, suggesting that the icELISA approach based on the arecoline specific antibody could be a widely applicable and easy operation method in detection of arecoline in foods and medicines.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
基于单克隆抗体的icELISA检测中药和新鲜槟榔果中槟榔碱的建立
槟榔碱是存在于槟榔果中的主要生物碱,是一种成瘾性物质,被列为2B类潜在人类致癌物。目前,槟榔碱的检测主要依赖于基于色谱的方法,这种方法耗时且昂贵。我们用槟榔碱作为半抗原制备了一种高特异性的槟榔碱单克隆抗体(mAb)。采用单克隆抗体- a5h12建立了一种间接竞争酶联免疫吸附试验(icELISA),用于检测中药和新鲜槟榔果中的槟榔碱。icELISA结果表明,槟榔碱的半数最大抑制浓度(IC50)为67.9 ng/mL,工作范围为10.1 ~ 502.6 ng/mL,检出限(LOD)为3.6 ng/mL。高效液相色谱(HPLC)证实了icELISA方法的准确性和工作范围,提示基于槟榔碱特异性抗体的icELISA方法在食品和药品中槟榔碱的检测中具有广泛的适用性和简便的操作方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Food and Agricultural Immunology
Food and Agricultural Immunology 农林科学-毒理学
CiteScore
5.30
自引率
6.70%
发文量
52
审稿时长
2 months
期刊介绍: Food and Agricultural Immunology is an international open access journal publishing original immunological research with applications in food, agricultural, environmental and veterinary science. Submissions describing the use of immunological techniques and methods are particularly welcomed. The journal aims to expand our understanding of the interactions at the interface of food and immune systems including studies on: -Development of diagnostic systems – all types of ligand-based assays, e.g. antibody, aptamer -Application of ligand-based assays for the detection or identification of molecules of interest in food science, agricultural research, veterinary investigations and clinical systems relating to food allergy or sensitivity to agricultural chemicals -Effects of food on the immune system -Studies on allergy and allergic reactions -Investigations into food allergies -Development of allergen-free food systems -Development of novel assay formats -Applications of assay systems to the monitoring of food items in relation to safety and labelling -Food quality issues, e.g. speciation, adulteration and contamination -Comparisons between different analytical techniques The journal publishes research and review articles and is essential reading for food scientists, immunologists and all those concerned with the interaction between food and immune systems.
期刊最新文献
Anti-inflammatory activity of Staphylea bumalda leaves through inhibiting ERK1/2 and NF-κB signalling, and activating Nrf2/HO-1 signalling in LPS-stimulated RAW264.7 cells Dietary fiber: an unmatched food component for sustainable health α-Amylase enzyme inhibition relevant to type II diabetes by using functional yogurt with Cinnamomum verum and Stevia rebaudiana Obesity and micronutrients deficit, when and how to suplement Effect of critical amino acids’ properties on potential allergenicity of Ara h 2 epitopes
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1