Recovery of pure C-phycoerythrin from a limestone drought tolerant cyanobacterium Nostoc sp. and evaluation of its biological activity

B. Nowruzi, H. Fahimi, A. Lorenzi
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引用次数: 11

Abstract

Se realizó la caracterización de ficoeritrina de la cepa A5 de Nostoc sp., seguida de investigación de su actividad biológica para aplicaciones biotecnológicas. Para la extracción de ficoeritrina, el uso de tampón acetato (pH 5.1) produjo 65.04 µg mL-1, y se identificó como C-ficoeritrina. Los resultados de su actividad antioxidante sugirieron su acción como un potente eliminador de radicales libres. Además, la C-ficoeritrina de Nostoc mostró una capacidad notable como agente antibacteriano y antifúngico, con estabilidad significativa de hasta 10 días. La glucosa (4 mg mL-1) fue un buen conservante para la C-ficoeritrina a 25 y 4 ºC. Se obtuvo por primera vez una C-ficoeritrina estable de Nostoc sp. en condiciones de sequía en piedra caliza, lo que demuestra la necesidad de estudiar microorganismos de ambientes extremos. The phycoerythrin characterization from Nostoc sp. strain A5 was done, followed by investigation of its biological activity for biotechnological applications. For phycoerythrin extraction, the use of acetate buffer (pH 5.1) resulted in 65.04 µg mL-1, and C-phycoerythrin was identified. Results of its antioxidant activity suggested action as a potent free radical scavenger. In addition, Nostoc's C-phycoerythrin showed noteworthy ability for antibacterial and antifungal agents with significant stability up to 10 days. Glucose (4 mg mL-1) was a good preservative for C-phycoerythrin at 25 and 4 ºC. A stable C-phycoerythrin from Nostoc sp. was obtained for the first time from limestone drought conditions, showing the need of studying microorganisms from extreme environments.
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从石灰石耐干旱蓝藻Nostoc sp.中回收纯c -藻红蛋白及其生物活性评价
对Nostoc sp. A5株藻红蛋白进行了表征,并对其生物活性进行了生物技术应用研究。用醋酸缓冲液(pH 5.1)提取藻红蛋白,得到65.04µg mL-1,鉴定为c -藻红蛋白。其抗氧化活性的结果表明,它是一种有效的自由基清除剂。此外,Nostoc的c -藻红蛋白作为一种抗菌和抗真菌药物表现出显著的能力,其显著的稳定性可达10天。葡萄糖(4 mg mL-1)在25℃和4℃时是C-藻红蛋白的良好防腐剂。本研究的目的是确定一种稳定的c -藻红蛋白,它是在干燥条件下从Nostoc sp.获得的,这表明有必要研究极端环境中的微生物。= =地理= =根据美国人口普查,这个县的面积为,其中土地面积为,人口密度为每平方英里(1 /平方公里)。= =地理= =根据美国人口普查,这个县的面积为,其中土地和(1.1%)水。= =地理= =根据美国人口普查,这个县的面积为。此外,Nostoc的C-phycoerythrin作为抗菌和抗真菌药物具有值得注意的性能,其显著稳定性可达10天。葡萄糖(4 mg mL-1)在25和4°C时是C-phycoerythrin的良好防腐剂。= =地理= =根据美国人口普查,这个县的面积为,其中土地面积为,其中土地面积为。
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审稿时长
12 weeks
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