Reliability of RT-qPCR Pooling Method for COVID-19 Detection in Various Cycle Threshold Values

Althea Medical Journal Pub Date : 2023-06-30 DOI:10.15850/amj.v10n2.2940

Muhammad Fauzan Alif Radjawali, M. Jihadah, L. Chaidir

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Abstract

Background: Reverse Transcriptase Quantitative Real-time Polymerase Chain Reaction (RT-qPCR) is a standard method to detect SARS-CoV-2, the cause of COVID-19 disease, albeit expensive for some laboratory settings. The pooling test is widely used for large-scale screening to speed up the turn-around time and reduce the cost of the RT-qPCR. However, the pooling test involves mixing a certain number of specimens which theoretically increases the possibility of false-negative results. This study aimed to evaluate the accuracy of the pooling test compared with the non-pooling test in different Ct values as a surrogate for viral load.Methods: RT-qPCR was performed in three groups of samples: non-pooling (individual samples), pooling of 5 samples and 11 samples, with various ranges of Ct value in the respective group: x<25 (n=4); 25

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RT-qPCR合并法在不同周期阈值下检测新冠肺炎的可靠性

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Althea Medical Journal

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20 weeks