{"title":"Development of Sunscreen Containing Alpha-Mangostin Riched Extract with Anti-Tyrosinase Activities","authors":"P. Tangyuenyongwatana, W. Gritsanapan","doi":"10.12982/cmujns.2022.064","DOIUrl":null,"url":null,"abstract":"Abstract This study focused on the development of extracts from mangosteen rinds for use as whitening and sunscreen agents. Mangosteen rinds were obtained from 9 locations in the East and the South of Thailand. Ethanol (95%) crude rind extracts were prepared and separated by column chromatography. The fractions were collected from top (F1-F4) and mid spots (F12-F15). These fractions were subjected to tyrosinase inhibitory activity assay and found that the top spot exhibited low activity with IC50 > 100 µg/mL while the mid spots inhibited tyrosinase enzyme at IC50 18.48 µg/mL which is more sensitive than a positive control, kojic acid (38.46 µg/mL). The mid spots fraction from the crude extract of Chanthaburi sample 1 was analyzed with HPLC and was assigned as MGS-1 extract which contained a high amount of α-mangostin. MGS-1 extract was formulated as oil in water cream with 2, 4, 6, and 9 %w/w extract. All creams were subjected to measure SPF and UVA/UVB ratio using an Optometrics SPF-290S analyzer. It was found that the cream with 6 %w/w MGS-1 extract showed the best results with SPF at 10.36 ± 1.07 and had a UVA/UVB ratio equal to 0.451. These sunscreen properties are close to the properties of Salisol-3 (benzophenone), a standard sunscreen. In conclusion, MGS-1 extract has a good potential to be used as a whitening and sunscreen agent in cosmetics with a lower cost of substrate because mangosteen rinds can be found as waste from eating mangosteen fruit every year in Thailand. Keywords: Mangosteen rind, MGS-1 extract, Tyrosinase inhibition, Whitening agent, Sunscreen","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chiang Mai University journal of natural sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12982/cmujns.2022.064","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Health Professions","Score":null,"Total":0}
引用次数: 0
Abstract
Abstract This study focused on the development of extracts from mangosteen rinds for use as whitening and sunscreen agents. Mangosteen rinds were obtained from 9 locations in the East and the South of Thailand. Ethanol (95%) crude rind extracts were prepared and separated by column chromatography. The fractions were collected from top (F1-F4) and mid spots (F12-F15). These fractions were subjected to tyrosinase inhibitory activity assay and found that the top spot exhibited low activity with IC50 > 100 µg/mL while the mid spots inhibited tyrosinase enzyme at IC50 18.48 µg/mL which is more sensitive than a positive control, kojic acid (38.46 µg/mL). The mid spots fraction from the crude extract of Chanthaburi sample 1 was analyzed with HPLC and was assigned as MGS-1 extract which contained a high amount of α-mangostin. MGS-1 extract was formulated as oil in water cream with 2, 4, 6, and 9 %w/w extract. All creams were subjected to measure SPF and UVA/UVB ratio using an Optometrics SPF-290S analyzer. It was found that the cream with 6 %w/w MGS-1 extract showed the best results with SPF at 10.36 ± 1.07 and had a UVA/UVB ratio equal to 0.451. These sunscreen properties are close to the properties of Salisol-3 (benzophenone), a standard sunscreen. In conclusion, MGS-1 extract has a good potential to be used as a whitening and sunscreen agent in cosmetics with a lower cost of substrate because mangosteen rinds can be found as waste from eating mangosteen fruit every year in Thailand. Keywords: Mangosteen rind, MGS-1 extract, Tyrosinase inhibition, Whitening agent, Sunscreen