Investigation of The Antioxidant Activity of Cinnamon Bark Extracted with Different Solvents

M. Afdal, A. Kasim, A. Alimon, N. Abdullah
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Abstract

The objective of this study was to evaluate the effect of methanol, ethyl acetate, and hexane for the extraction of cinnamon bark (Cinnamomum burmanni) (CB) on the contents of the total phenolic, total flavonoid and its 1,1,-diphonyl-1-picryl-hydrazyl (DPPH) radical scavenging activity. CB samples were ground with grinder machine in one mm sieve and extracted with treatments to determine parameter measured. All CB samples were extracted using different treatments. Parameter measured were total phenolic, total flavonoid and DPPH. CB was ground and extracted by using three treatments, data collected was statistical analysis and followed with Duncant Test, The highest total phenolic content found when CB extracted with methanol with the value of 111.43 mg gallic acids equivalent (GAE)/g DW while the lowest amount detected in hexane with the amount of 57.23 mg GAE/g dry weight (DW). It was significantly different (P<0.05) from those extracted with ethyl acetate and hexane. There was significant (P<0.05) different for the content of flavonoid among all treatment groups. Methanol was the highest solvent and hexane was the lowest with the amount of 286.27 and 64.73 mg rutin equivalent (RE)/g DW respectively. There was significantly different (P<0.05) for scavenging activity among all treatment. Using methanol in extracting CB showed the highest scavenging activity of 69.59 % among all other treatment groups. It could be concluded that Methanol was the best solution in extracting CB
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不同溶剂提取肉桂皮抗氧化活性的研究
本研究的目的是评价甲醇、乙酸乙酯和己烷提取肉桂皮(肉桂)对总酚、总黄酮含量及其1,1,-二苯基-1-丙酰肼(DPPH)自由基清除活性的影响。CB样品用研磨机在1mm筛中研磨,并通过处理提取以确定测量的参数。使用不同的处理提取所有CB样品。测定的参数为总酚、总黄酮和DPPH。CB通过三种处理进行研磨和提取,收集的数据进行统计分析,然后进行Duncant试验。当用甲醇提取CB时,发现最高的总酚含量为111.43 mg没食子酸当量(GAE)/g DW,而在己烷中检测到的最低含量为57.23 mg GAE/g干重(DW)。与用乙酸乙酯和己烷提取的样品有显著差异(P<0.05)。各处理组黄酮含量差异有显著性(P<0.05)。甲醇是最高的溶剂,己烷是最低的溶剂,其量分别为286.27和64.73 mg芦丁当量(RE)/g DW。各处理间清除活性差异有显著性(P<0.05)。在所有其他处理组中,用甲醇提取CB的清除活性最高,为69.59%。结果表明,甲醇是提取CB的最佳溶液
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