Intra-specific Variability in Anti-Diabetic Activity and UPLC Quantification of Oleanolic Acid from Two Morphotypes and Three Cytotypes of Achyranthes aspera

Pub Date : 2022-03-04 DOI:10.1080/22311866.2022.2052963

N. Sharma, B. Singh, A. Bhatia, M. Wani, R. C. Gupta

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引用次数: 1

Abstract

Abstract Achyranthes aspera is an economically important medicinal plant, exhibiting a wide range of cytomorphological diversity. Taking into account, its pharmacological activities, as well as variability, found within the species, the present attempt was made to study the detailed meiotic course in 13 plant samples collected from different areas of North India. The anti-diabetic activity of three different cytotypes (diploid, tetraploid, and hexaploid) and two different morphotypes (obovate-leaf morphotype and ovate-leaf morphotype) was investigated in alloxan-induced Swiss albino male rats. Additionally, phytochemical analysis of aerial parts of these cytotypes and morphotypes was performed using the UPLC technique. The meiotic analysis depicts the presence of three cytotypes with 2n=14, 28, and 42. The results indicated that the diploid cytotype of A. aspera has maximum activity against diabetes in alloxan-induced diabetic rats. Out of the two morphotypes, the obovate-leaf morphotype showed a potent anti-diabetic effect as compared to the ovate-leaf morphotype. UPLC results showed that the maximum percentage of active component oleanolic acid was present in diploid cytotype followed by tetraploid and hexaploid cytotypes. A comparative study of the two morphotypes within the tetraploid cytotype revealed that the obovate-leaf morphotype possessed a higher percentage of oleanolic acid as compared to the ovate-leaf morphotype. Graphical abstract

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两种形态类型和三种细胞类型的牛膝抗糖尿病活性的特异内变异性和齐墩果酸的UPLC定量

摘要牛膝是一种重要的经济药用植物，具有广泛的细胞形态多样性。考虑到其药理活性以及在该物种中发现的变异性，本次尝试研究了从北印度不同地区采集的13个植物样本的详细减数分裂过程。研究了三种不同细胞类型（二倍体、四倍体和六倍体）和两种不同形态类型（倒卵形叶形态和卵形叶形态）在四氧嘧啶诱导的瑞士白化雄性大鼠中的抗糖尿病活性。此外，使用UPLC技术对这些细胞类型和形态类型的地上部分进行了植物化学分析。减数分裂分析描述了2n＝14、28和42的三种细胞类型的存在。结果表明，在四氧嘧啶诱导的糖尿病大鼠中，A.aspera的二倍体细胞型具有最大的抗糖尿病活性。在这两种形态中，倒卵形叶形态与卵形叶形态相比显示出强大的抗糖尿病作用。UPLC结果显示，活性成分齐墩果酸的最大百分比存在于二倍体细胞型中，其次是四倍体和六倍体细胞型。对四倍体细胞型中两种形态类型的比较研究表明，与卵状叶形态类型相比，倒卵形叶形态类型具有更高百分比的齐墩果酸。图形摘要

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