Ghrelin (GHRL) Gene Polymorphism and its Association with Growth and Body Size Parameters in Three Nigerian Chicken Breeds

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies Pub Date : 2021-05-14 DOI:10.15835/BUASVMCN-ASB:2020.0019

A. Sanda, M. Bemji, M. Wheto, A. Oso, M. O. Sanda, O. Olowofeso

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Abstract

This study was conducted to identify ghrelin (GHRL) gene polymorphism within exons 2 and 3 in three chicken breeds and to determine its association with growth and linear body measurements. Three hundred one-day-old chicks comprising 100 each of three chicken breeds (FUNAAB Alpha, Noiler and Shika Brown) were used for the research and were raised for eighteen weeks. Fifty birds per breed were sampled for blood collection and Genomic DNA was extracted using Zymo miniprep kit. DNA was amplified and PCR products digested with Eco72I restriction enzyme. Growth data were analysed using Generalized Linear Model of SAS. Noiler had the highest (P <0.05) growth traits from 10 to 18 weeks among studied breeds. GHRL gene polymorphism had no significant (P >0.05) effect on growth traits. However, interaction between GHRL gene polymorphism and chicken breeds revealed that Noiler chickens AA and AB had the best (P <0.05) productive performance from 2 to 18 weeks.

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3个尼日利亚鸡品种生长素(GHRL)基因多态性及其与生长和体型参数的关系

本研究旨在鉴定三种鸡品种的ghrelin (GHRL)基因外显子2和3的多态性，并确定其与生长和线性体测量的关系。研究使用了300只1日龄的雏鸡，其中3个品种(FUNAAB Alpha、Noiler和Shika Brown)各100只，饲养18周。每个品种50只采集血样，使用Zymo迷你试剂盒提取基因组DNA。DNA扩增，PCR产物用Eco72I酶切。生长数据采用SAS广义线性模型进行分析。黑鸡对生长性状影响最大(p0.05)。然而，GHRL基因多态性与鸡品种的互作表明，AA和AB在2 ~ 18周龄的生产性能最好(P <0.05)。

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Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies

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8 weeks