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Potentials of Exogenous Melatonin Administration on Growth and Performance of West African Dwarf bucks 外源褪黑素对西非矮雄鹿生长和生产性能的影响
T. J. Williams, Olasupo Idris Yusuf, I. James, Olubukonla Ajike Isah, N. Okwelum, O. Oke, J. Daramola
The effect of exogenous melatonin (EM) on growth, physiological and haematological parameters of West African dwarf (WAD) bucks was evaluated in the experiment. Intact selected sixteen WAD bucks were distributed into 4 treatment groups of 0, 3, 6 and 9 mg EM. Bucks were orally dozed with EM 3 times per week for 16 weeks. Blood samples were collected at 0th week, 8th week and 16th week of experiment for evaluation of haematological parameters. Analysis of Variance was carried out and significant means were separated using SAS. Results showed bucks given 6 mg EM had higher average daily weight gain of 31.3 g compared with others. Feed and water intake were similar across treatments, but feed conversion ratio was significantly lower in bucks given 6 mg EM. Values recorded in the morning of data collection days showed pulse and respiratory rates to be significantly different, with bucks given 6 mg of EM having lowest values.  6 mg group was also different for packed cell volume (32.57%), haemoglobin (10.72g/dl), red blood cell (11.38 1012 /L), neutrophil (35.50%) and lymphocyte (64.30%). Therefore, dose of 6 mg/animal EM influences growth performance, physiological and haematological parameters of WAD bucks positively and is therefore recommended for WAD bucks for optimal performance.
本实验评价了外源性褪黑素(EM)对西非矮子(WAD)生长、生理和血液学参数的影响。选择16只完整的WAD雄鹿分为0、3、6和9mg EM 4个治疗组。雄鹿每周口服EM 3次,持续16周。在实验的第0周、第8周和第16周采集血样,以评估血液学参数。进行方差分析,并使用SAS分离显著均值。结果显示,与其他雄鹿相比,给予6 mg EM的雄鹿平均每日体重增加31.3 g。不同处理的饲料和水分摄入相似,但给予6 mg EM的雄鹿的饲料转化率明显较低。数据收集日上午记录的值显示脉搏和呼吸频率显著不同,给予6 mg的EM的雄鹿具有最低值。6mg组的填充细胞体积(32.57%)、血红蛋白(10.72g/dl)、红细胞(11.38 1012/L)、中性粒细胞(35.50%)和淋巴细胞(64.30%)也不同。
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引用次数: 0
Effect of Lactoferrin on Paraoxonase Activity, Some Acute Phase Proteins and Oxidant/Antioxidant System 乳铁蛋白对对对氧磷酶活性、某些急性期蛋白质及氧化/抗氧化系统的影响
M. Ozturkler, O. Atakisi
Lactoferrin is a member of the high affinity to glycoprotein family of transferrin for iron and plays a role in natural immunity as well as antibacterial, antifungal, antiviral, antiparasitic, antioxidant, anticancer, antiinflammatory, bone health and immunomodulation. The aim this study was to investigate of the acute effect of lactoferrin on paraoxonase activity, oxidant/antioxidant system and total protein, albumin and globulin. This study was carried out on 6-month Sprague Dawley rats in a total number of 20 subjects. Paraoxonase activity, total antioxidant capacity (TAC), total oxidant capacity (TOC), total protein, albumin and globulin levels were measured through the spectrophotometer. When the lactoferrin group was compared with the control group, it was found that TAC levels were statistically higher (P<0.05), TOC levels were statistically lower (P<0.01) and paraoxonase activity was statistically higher (P<0.05). There was no difference in total protein and globulin levels in the lactoferrin group compared to the control group. As a result, acute lactoferrin administration may play an important role in the regulation of antioxidant balance by increasing paraoxonase activity and decreasing the level of reactive oxygen species.
乳铁蛋白是铁对转铁蛋白糖蛋白家族的高亲和力成员,在自然免疫以及抗菌、抗真菌、抗病毒、抗寄生虫、抗氧化、抗癌、抗炎、骨骼健康和免疫调节中发挥作用。本研究的目的是研究乳铁蛋白对对对氧磷酶活性、氧化/抗氧化系统以及总蛋白、白蛋白和球蛋白的急性影响。本研究在总共20名受试者中对6个月大的Sprague-Dawley大鼠进行。用分光光度计测定对氧磷酶活性、总抗氧化能力(TAC)、总氧化能力(TOC)、总蛋白、白蛋白和球蛋白水平。乳铁蛋白组与对照组比较,TAC水平显著升高(P<0.05),TOC水平显著降低(P<0.01),对氧磷酶活性显著升高(P<0.01)。乳铁蛋白组总蛋白和球蛋白水平与对照组相比无差异。因此,急性乳铁蛋白给药可能通过提高对氧磷酶活性和降低活性氧水平,在调节抗氧化平衡中发挥重要作用。
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引用次数: 0
Haemoparasite Prevalence, Genetic Diversity of TLR2B Gene and Relationship with Haematological Parameters in Chicken 鸡血寄生虫流行、TLR2B基因遗传多样性及其与血液学参数的关系
B. Ilori, M. Adeleke, Adebisi Adeleye, D. Oguntade, S. Durosaro, Olufemi Adekunle Osonowo, M. Takeet, Kayode Akano, Henry Temitope Akinloluwa Ojoawo, M. Ozoje
Haemoparasite constitute a major challenge in native chicken production in Africa. This study determines the genetic diversity and the effect of TLR2B gene polymorphism on haemoparasite and haematology of the chickens based on genotype and sex. 600 chickens of 25 weeks old consisting of Naked neck (NN), Normal feather (NF), and the Frizzle feather (FF) reared in battery cage-system were sampled for blood and analyzed for haematology, parasite occurrence and load, polymerase chain reaction, and gene sequencing. Polymorphisms were detected and their effect on haematology was determined. Results showed the occurrence of Plasmodium gallinacieum, Trypanosoma brucei, and Leucocytozoon schoutedeni with NF having the highest occurrence followed by NN and FF chicken genotype. There was a significant (P <0.05) effect of genotype and sex on haematology. Seven of the eight polymorphism detected were singleton and found only in NF while parsimonious 656GA was detected in all the chicken genotypes with no relationship with haematology and haemoparasite. NF had the highest nucleotide (0.00114) and haplotype diversity (0.584). The study revealed the occurrence of genetic variation in TLR2B gene, haematology, and haemoparasite in FUNAAB Alpha chickens which could provide baseline information in future breeding programmes of the chicken for the tropical environment.
血寄生虫是非洲土鸡生产面临的主要挑战。本研究根据基因型和性别确定了TLR2B基因多态性对鸡血寄生虫和血液学的遗传多样性和影响。选取25周龄裸颈(NN)、正常羽(NF)和毛毛羽(FF)鸡600只,进行血流变学、寄生虫发生和负荷、聚合酶链反应和基因测序分析。检测多态性并确定其对血液学的影响。结果鸡基因型中有鸡源性疟原虫、布鲁氏锥虫和肖氏白细胞虫,其中NF型发生率最高,其次是NN型和FF型。基因型和性别对血液学有显著影响(P <0.05)。检测到的8个多态性中有7个为单态,仅在NF中发现,而所有鸡基因型中均检测到简约型656GA,与血液病和血寄生虫无关。NF具有最高的核苷酸(0.00114)和单倍型多样性(0.584)。该研究揭示了FUNAAB α鸡中TLR2B基因、血液学和血寄生虫遗传变异的发生,为未来热带环境下的鸡育种计划提供了基础信息。
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引用次数: 0
Larvicidal Efficacy of a Tropical Seaweed (Dictyota ciliolata) Extracts on Vectorial Mosquito Species and Non-Target Effect on Guppy Fish (Poecilia reticulata) 热带海藻(Dictyota ciliolata)提取物对媒介蚊种的杀幼虫效果和对Guppy Fish(Poeclia reticulata)的非靶向作用
F. Adetoro, M. Lawal, O. W. Elegbeleye, Oluwadamilola Ruqayyah Okubadejo, Anthony Oladipupo Truscott, Murisiq Olalekan Adegbite
Extracts of seaweeds are promising candidates for eco-friendly, safe, and biodegradable larvicides and as alternatives to chemical larvicides used in mosquito control strategies. This study evaluated the larvicidal effects of Dictyota ciliolata on the mosquito larvae of Aedes aegypti, Anopheles gambiae, and Culex quinquefasciatus. The seaweed was extracted using ethanol and methanol. The larvicidal assays were conducted per WHO standards by exposing twenty 4th instar larvae to 100, 200, 300, 400, 500, and 600 mg/L of the derived extracts. The LC50 and LC95 values were obtained by calculating the probit values of the observed mortalities. LC50 of the ethanolic and methanolic extracts of Dictyota ciliolata against Anopheles gambiae larvae were 238.45 mg/L and 497.28 mg/L respectively; 179.02 mg/L and 224.96 mg/L respectively against Aedes aegypti, and 167.49 mg/L and 226.69 mg/L respectively for Culex quinquefasciatus. The results established that ethanolic and methanolic seaweed extracts were most influential on Culex quinquefasciatus followed by Aedes aegypti and Anopheles gambiae. Although mortalities were recorded for Poecilia reticulata, such may not be enough to de-emphasize the observed larvicidal potentials of Dictyota ciliolata as some other factors could have been responsible for the said mortalities in the non-target species.
海藻提取物是环保、安全和可生物降解的杀幼虫剂的有前途的候选者,也是蚊子控制策略中使用的化学杀幼虫药的替代品。本研究评估了纤毛网柄菌对埃及伊蚊、冈比亚按蚊和致倦库蚊幼虫的杀幼虫作用。海藻是用乙醇和甲醇提取的。根据世界卫生组织标准,将24龄幼虫暴露于100、200、300、400、500和600mg/L的衍生提取物中,进行杀幼虫试验。LC50和LC95值是通过计算观察到的死亡率的概率值来获得的。纤毛网狼乙醇提取物和甲醇提取物对冈比亚按蚊幼虫的LC50分别为238.45mg/L和497.28mg/L;对埃及伊蚊分别为179.02 mg/L和224.96 mg/L,对致倦库蚊分别为167.49 mg/L和226.69 mg/L。结果表明,乙醇和甲烷海藻提取物对致倦库蚊的影响最大,其次是埃及伊蚊和冈比亚按蚊。尽管记录了网状网斑狼的死亡率,但这可能不足以减轻观察到的纤毛网斑狼杀幼虫的潜力,因为一些其他因素可能是导致非目标物种死亡的原因。
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引用次数: 0
Pig Carcass Quality Evolution in Romania 罗马尼亚猪胴体品质的演变
I. Ladoşi, M. Visan, Paula Călianu, D. Ladoși
The Romanian Carcass Classification Commission (RCCC) was established in 2004 and it became the official partner for delivering carcass quality information to the European Commission in 2007. RCCC is the NGO serving the interests of the private stakeholders in the meat chain (farmers, slaughterhouses, processors, and traders), which guarantees the neutrality and objectivity of carcass quality assessment according to SEUROP grading system’s legally binding rules. Considering the importance of an independent assessment of the slaughter pig quality for the entire food chain, the analysis of the carcass quality evolution in Romania is important for future benchmarking and trend assessments as well. The current review is based on the public data developed in time by the RCCC. It demonstrates the importance of a professional organization measuring the quality of carcass in slaughtered pigs and its impact on the meat chain as a whole, both for its stakeholders and consumers. It also highlights the evolution of the pig carcasses produced in Romania, underlining the main factors involved and expected trends under the current circumstances of the presence and spread of the African Swine Fever virus (ASF) within Romania in the last four years. The main conclusion is that while the carcass quality improved over time, the graded carcass number decreased after 2017, primarily due to ASF outbreaks in commercial farms and the overall reduction of the farmed pig inventory, and increasing dependence of Romanian consumers on imports.
罗马尼亚胴体分类委员会(RCCC)成立于2004年,并于2007年成为向欧盟委员会提供胴体质量信息的官方合作伙伴。RCCC是一家为肉类链中的私人利益相关者(农民、屠宰场、加工商和贸易商)的利益服务的非政府组织,根据SEUROP分级系统具有法律约束力的规则,保证胴体质量评估的中立性和客观性。考虑到独立评估屠宰猪质量对整个食物链的重要性,罗马尼亚屠体质量演变的分析对未来的基准和趋势评估也很重要。目前的审查是以加拿大皇家骑警及时制定的公共数据为基础的。它表明了专业组织衡量屠宰猪胴体质量及其对整个肉类链的影响的重要性,无论是对其利益相关者还是消费者。它还强调了罗马尼亚生产的猪胴体的演变,强调了在过去四年中非洲猪瘟病毒在罗马尼亚境内存在和传播的当前情况下所涉及的主要因素和预期趋势。主要结论是,虽然胴体质量随着时间的推移而改善,但分级胴体数量在2017年后有所下降,主要是由于商业农场爆发的ASF疫情和养殖生猪库存的总体减少,以及罗马尼亚消费者对进口的依赖性增加。
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引用次数: 0
Phenotypic Characterization of Sheep Population in Aures, Hodna and Ziban Zones, Algeria 阿尔及利亚Aures、Hodna和Ziban地区绵羊种群的表型特征
Kenza Mohammedi, Z. Laiadi, Mohamed Titaouine
This study aims to characterize the sheep population phenotypically in three zones in northeastern Algeria: Aures, Hodna and Ziban. A total of 160 female animals of 2 to 4 years of age were subject to 4 quantitative linear body measurements (body length BL, withers height WH, tail length TL and wool weight WW) and 9 qualitative physical traits (head length HL, ear orientation EO, horn presence H, neck length NL, wool extent WE, tail texture TT, eyes shape ES, head color HC and wool color WC). The results indicate that the sheep population in these areas belonged to the Ouled Djellel sheep breed with some abnormal phenotypes, which refers to uncontrolled crossbreeding. Body measurements show moderate variation coefficient values, with the highest value reported for WW (7.37%). However, despite the weak correlations found in this study, clear ones were revealed between WH and TL (0.30) and BL and WW (0.26). The Hierarchical Agglomerative Clustering analysis sub-divided the sheep population into three classes, in which an overlap, according to body measurements, was remarked between Hodna and Ziban and between Aures and Ziban but not between Aures and Hodna.
本研究旨在描述阿尔及利亚东北部三个地区的绵羊种群表型:Aures、Hodna和Ziban。总共对160只2-4岁的雌性动物进行了4次定量线性身体测量(体长BL、肩高WH、尾部长度TL和羊毛重量WW)和9个定性身体特征(头部长度HL、耳朵方向EO、角存在H、颈部长度NL、羊毛范围WE、尾部纹理TT、眼睛形状ES、头部颜色HC和羊毛颜色WC)。结果表明,这些地区的绵羊群体属于Ouled Djellel绵羊品种,具有一些异常表型,即不受控制的杂交。身体测量显示出中等的变异系数值,WW的报告值最高(7.37%)。然而,尽管本研究发现相关性较弱,但WH和TL(0.30)以及BL和WW(0.26)之间的相关性很明显。分层聚集聚类分析将绵羊种群分为三类,根据身体测量,在霍德纳和齐班之间以及在奥雷斯和齐班之间都有评论,但奥雷斯和霍德纳之间没有评论。
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引用次数: 0
Evaluation of Microclimate Parameters, the Interdependence Between Them on Two Types of Bedding in the Broiler Houses 肉鸡舍小气候参数的评价及其与两种垫层的相关性
C. Hegedus, S. Pașcalău, Luisa Andronie, A. Rotaru
The research was carried out in broiler houses coming from a commercial broiler farm. Two consecutive series of broiler were followed for each of the specific housing conditions in terms of bedding quality: straw: series 1 and 2 in shelter H4 and bedding mixture: ¼ straw and the rest sawdust for series 1 and 2 housed in shelter H10. The microclimate parameters were monitored daily in three different areas, the value of the three readings representing the average. To reduce the pH of the bedding and inclusive of the removal of ammonia resulting from the degradation of organic matter in the bedding, it is returned 2-3 times a week. The results reveal that: there are no exceedances of the values in terms of physical parameters of microclimate, between them there are extremely significant correlations (positive or negative). The correlation between the temperature of the straw bedding and the carbon dioxide in the shelter air is extremely significant and insignificant at the level of the mixed bedding. The pH of the bedding has lower values in the case of the straw ¼+ sawdust mixture by turning the bedding 2-3 times a week. The temperature value at the bedding level is lower in the case of straw compared to the mixture of straw ¼+ sawdust (29.69±2.38 0C - 26.35±2.64 0C), which entails significant differences (*P<0.05). Between the quality of the bedding in terms of pH and the amount of ammonia monitored at the level of the houses, there is an extremely close negative relationship: (-0.83*** ppm NH3), straw bedding and (-0.96*** ppm NH3) straw ¼+ sawdust mixture bedding. Conclusion: keeping the microclimate parameters under control by ensuring the necessary air volume, monitoring the pollutants but also the physical microclimate parameters, regular return of bedding have as a result low mortality rate and low percentage of ammonia burns which denotes ensuring good welfare conditions.
该研究是在来自一个商业肉鸡农场的肉鸡房中进行的。根据不同的饲养条件,对连续两个系列的肉仔鸡进行床上用品质量的跟踪:在棚舍H4中,稻草为1和2系列;在棚舍H10中,1和2系列的床上用品混合物为1 / 4稻草和剩余木屑。每天监测三个不同地区的小气候参数,三个读数的值代表平均值。为了降低垫层的pH值,包括去除垫层中有机物降解产生的氨,每周返回2-3次。结果表明:小气候各物理参数均不存在异常值,它们之间存在极显著的正相关或负相关。在混合层理水平上,秸秆层理温度与防护林空气中二氧化碳含量的相关性极显著,不显著。每周翻动床上用品2-3次,秸秆+木屑混合物的床上用品pH值较低。秸秆层理层温度值(29.69±2.38℃~ 26.35±2.64℃)低于秸秆+木屑混合层理层温度值(*P<0.05)。层理质量的pH值与屋级氨监测量呈极密切的负相关关系:(-0.83*** ppm NH3)、秸秆层理和(-0.96*** ppm NH3)秸秆¼+木屑混合层理。结论:通过控制小气候参数,保证必要的风量,监测污染物和物理小气候参数,定期归还被褥,可以降低死亡率和氨烧伤率,确保良好的福利条件。
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引用次数: 0
Methods of Improving Reproductive Parameters in Sheep and The Major Genes Associated with Prolificacy: A Review 绵羊生殖参数改良方法及与繁殖能力相关的主要基因研究进展
Alexandru Marius Deac, A. Muscă, Marius Gavril Aipatioaie, V. Coșier, M. Zăhan
Farm profitability is heavily influenced by reproductive capacity. Fertility, prolificacy, and fecundity are all indicators of reproductive efficiency. In sheep with high economic value, prolificacy is a key reproduction parameter (Notter, 2008). Because most sheep breeds are monotocous, similar to Mouflon wild sheep (Garel et al., 2005), improving fecundity is a serious concern (Tang et al., 2019). This review aims to study genes and the genetic means of improving sheep reproduction parameters. Numerous mutations in the transforming growth factor (TGF) superfamily have been reported to influence sheep reproductive parameters. As a result, molecular genetics and marker-assisted selection (MAS) are essential in improving reproduction efficiency. If these mutations are not present in the population, introgression of the beneficial mutations to indigenous breeds is possible. Because within-breed selection has been considered relatively inefficient, due to the low heritability of the trait, crossbreeding of native breeds with prolific breeds has been the major means of genetically improving prolificacy. Studying fecundity genes is important in order to increase production efficiency and stabilizing optimal litter sizes. Different studies based on whole-genome sequencing (WGS), which are called genome-wide association studies (GWAS), and also proteomic studies, transcriptome analysis, and mitochondrial DNA analysis have revealed further genetic variation with medium or minor effects on reproduction.
农场的盈利能力在很大程度上受到生殖能力的影响。生育力、多产性和繁殖力都是生殖效率的指标。在具有高经济价值的绵羊中,繁殖能力是一个关键的繁殖参数(Notter, 2008)。由于大多数羊品种都是单株的,类似于Mouflon野生羊(Garel等人,2005),因此提高繁殖力是一个严重的问题(Tang等人,2019)。本文旨在研究改善绵羊繁殖参数的基因及其遗传手段。据报道,转化生长因子(TGF)超家族的许多突变会影响绵羊的生殖参数。因此,分子遗传学和标记辅助选择(MAS)是提高繁殖效率的重要手段。如果这些突变在种群中不存在,则有益突变可能渗入本地品种。由于品种内选择被认为是相对低效的,由于性状的低遗传力,本地品种与多产品种的杂交一直是遗传上提高多产性的主要手段。研究繁殖力基因对提高生产效率和稳定最佳产仔数具有重要意义。基于全基因组测序(WGS)的不同研究,即全基因组关联研究(GWAS),以及蛋白质组学研究、转录组学分析和线粒体DNA分析,揭示了对生殖有中等或轻微影响的进一步遗传变异。
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引用次数: 0
Guidelines and Implications of Fish Slaughtering in the Ensuring of Welfare and Product Quality 鱼类屠宰在确保福利和产品质量方面的指导方针和意义
A. Boaru, D. Struți, B. Georgescu
The constant and accelerated increase of world fish production through the expansion of fisheries and the development of aquaculture has also generated a higher interest in animal welfare. In the present, is proven that the fish welfare is a factor that influences the quality of the product and the practices of capture and slaughter, which can compromise these attributes. The harvesting and suppressing life methods give to the biochemical processes of fish meat some particularities, as a result of the stressor to which it is subjected by tracking, agglomeration, space restriction and asphyxiation. In the view of all, it is important to correctly identify procedures that improve both the welfare of the fish and the quality of the product. In this review, there are highlighted some of the stunning and slaughter methods of fish used in the aquaculture or capture fisheries, focusing on the human agreed standards and international organizations. There are recommendations in this sense for operations prior to slaughter and the possibility of developing and implementing new methods, by country and species of fish. In this sense, the brain puncture stands out as a more humane method of slaughtering fish, which also improves the quality of the product.
由于渔业的扩大和水产养殖的发展,世界鱼类产量不断加速增长,这也使人们对动物福利产生了更高的兴趣。目前,已经证明鱼类福利是影响产品质量的一个因素,捕捞和屠宰的做法可能会损害这些属性。由于受到跟踪、聚集、空间限制和窒息等应激源的影响,采收和抑生方法使鱼肉的生化过程具有一定的特殊性。在所有人看来,正确确定既能提高鱼类福利又能提高产品质量的程序是很重要的。在本综述中,重点介绍了水产养殖或捕捞渔业中使用的一些鱼类的屠宰和屠宰方法,重点介绍了人类商定的标准和国际组织。在这个意义上,对屠宰前的操作以及按国家和鱼类种类制定和实施新方法的可能性提出了建议。从这个意义上说,脑穿刺作为一种更人道的屠宰鱼的方法脱颖而出,这也提高了产品的质量。
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引用次数: 0
Determination and Quantification of Some Organochlorines, Organophosphorus, Carbamates and Pyrethroids Pesticide Residues in Fresh and Frozen Fish in Jordan 约旦新鲜和冷冻鱼类中有机氯、有机磷、氨基甲酸酯类和拟除虫菊酯类农药残留量的测定和定量
A. Alaboudi, D. Almashhadany, B. S. Jarrah
Contamination of aquatic systems with pesticides occurs frequently. This study aimed to detect the levels of some pesticides: organochlorines, organophosphorus, pyrethroids, and carbamates groups in imported frozen and local fresh fish in Jordan. A total of 218 samples were randomly collected (194 imported frozen fish). Samples were analyzed using Liquid Chromatography with Tandem Mass Spectrometry (LC/Ms/Ms). Results showed that 23 different pesticides were found in this study. All 218 fish samples were contaminated with at least 4 compounds and maximally 12 pesticides. In terms of contamination rate, no significant differences between local and imported types were observed. Up to 65.2% of pesticides were detected at concentration higher than the maximum residue limit set by the WHO (10 ppb) in 215 samples (98.6%). These samples concluded heavy contaminations with different pesticides for either imported or locally raised products. Therefore, routine monitoring of pesticides in fish and their environment are officially required.
农药对水生系统的污染经常发生。本研究旨在检测约旦进口冷冻鱼和当地鲜鱼中有机氯、有机磷、拟除虫菊酯和氨基甲酸酯类杀虫剂的含量。随机抽取218份样本(其中194份为进口冷冻鱼)。采用液相色谱串联质谱法(LC/Ms/Ms)对样品进行分析。结果表明,本研究共检出23种不同的农药。所有218份鱼样本都被至少4种化合物和最多12种农药污染。在污染率方面,本地种类与进口种类并无显著差异。215个样本(98.6%)中,高达65.2%的农药浓度高于世界卫生组织规定的最大残留限量(10 ppb)。这些样本显示,进口或本地生产的产品均受到不同杀虫剂的严重污染。因此,官方要求对鱼类及其环境中的农药进行常规监测。
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引用次数: 0
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Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies
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