Effects of Agaricus blazei acidic polysaccharide on the aging of mice through keap1-Nrf2/ARE and MAPKs signal pathway

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Electronic Journal of Biotechnology Pub Date : 2022-05-01 DOI:10.1016/j.ejbt.2022.03.004

Xiao Guo , Yujie Ye , Xinzhu Liu , Yu Sheng , Ying Yu , Yingying Yang , Mingliu Gu , Rui Lin , Baohui Wang , Liping An , Xuechun Lu

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引用次数: 3

Abstract

Background

In view of the increasing human life and the aging of the population, the search for safe anti-aging drugs has become a hot topic. Agaricus blazei Murrill is a rare edible fungus, with a variety of biological activities. The purpose of this study was to clarify the anti-aging effect and mechanism of ABM-A on the aging induced by D-Galactose in mice.

Results

The result showed that ABM-A contained 87.2% of glucose, 3.3% of galactose, 3.8% mannose and 5.7% gluconic acid. The behavior of mice in the treatment group was significantly improved after administration of ABM-A. And the activity of SOD and CAT and the level of T-AOC were increased (p < 0.05), the content of MDA and ROS was decreased (p < 0.05) in the serum of mice in ABM-A group. The results of mechanism research showed that nine genes were screened out by functional annotation and enrichment analysis for the verification by RT-qPCR, and the results of RT-qPCR were consistent with those of RNA-seq. Western Blot results showed that ABM-A upregulated the expression of Hmox1, Myd88, p-c-Jun, Apc, Bmil, Cox7a2l and Ndufv1, down-regulated the expression of Nfe2l2, Keap1, Apoe, Mapk1and Atp1a3, and decreased the phosphorylation of p38 and Jnk, suggesting that it may play an anti-aging effect by regulating Nrf2/ARE and MAPKs superfamily signal pathways.

Conclusions

ABM-A can reduce oxidation reaction and play an anti-aging role through Keap1-Nrf2/ARE and MAPKs signaling pathway.

How to cite: Guo, X, Ye Y, Liu X, et al. Effects of Agaricus blazei acidic polysaccharide on the aging of mice through keap1-Nrf2/ARE and MAPKs signal pathway. Electron J Biotechnol 2022;57. https://doi.org/10.1016/j.ejbt.2022.03.004

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姬松茸酸性多糖通过keap1-Nrf2/ARE和MAPKs信号通路对小鼠衰老的影响

随着人类寿命的延长和人口的老龄化，寻找安全的抗衰老药物已成为一个热门话题。姬松茸(Agaricus blazei Murrill)是一种稀有食用菌，具有多种生物活性。本研究旨在阐明ABM-A对d -半乳糖致小鼠衰老的抗衰老作用及其机制。结果ABM-A中葡萄糖含量为87.2%，半乳糖含量为3.3%，甘露糖含量为3.8%，葡萄糖酸含量为5.7%。治疗组小鼠给药后行为学有明显改善。SOD、CAT活性升高，T-AOC水平升高(p <0.05)， MDA和ROS含量降低(p <0.05)。机制研究结果显示，通过功能注释和富集分析筛选出9个基因进行RT-qPCR验证，RT-qPCR结果与RNA-seq结果一致。Western Blot结果显示，ABM-A上调Hmox1、Myd88、p-c-Jun、Apc、bil、Cox7a2l和Ndufv1的表达，下调Nfe2l2、Keap1、Apoe、mapk1和Atp1a3的表达，降低p38和Jnk的磷酸化，提示其可能通过调节Nrf2/ARE和MAPKs超家族信号通路发挥抗衰老作用。结论sabm -a可通过Keap1-Nrf2/ARE和MAPKs信号通路抑制氧化反应，发挥抗衰老作用。引用方式:郭，X，叶艳，刘鑫，等。姬松茸酸性多糖通过keap1-Nrf2/ARE和MAPKs信号通路对小鼠衰老的影响。中国生物医学工程学报(英文版);2009;16。https://doi.org/10.1016/j.ejbt.2022.03.004

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来源期刊

Electronic Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology. The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th). The following areas are covered in the Journal: • Animal Biotechnology • Biofilms • Bioinformatics • Biomedicine • Biopolicies of International Cooperation • Biosafety • Biotechnology Industry • Biotechnology of Human Disorders • Chemical Engineering • Environmental Biotechnology • Food Biotechnology • Marine Biotechnology • Microbial Biotechnology • Molecular Biology and Genetics •Nanobiotechnology • Omics • Plant Biotechnology • Process Biotechnology • Process Chemistry and Technology • Tissue Engineering