Differential cytotoxic effects of fenbendazole on mouse lymphoma EL-4 cells and spleen cells

Hae-Jung Jung, You-Jeong Lee, H. Joo
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Abstract

Fenbendazole (FBZ) is a benzimidazole anthelmintic widely used to treat parasitic infections. The anticancer effect of FBZ has been recently highlighted leading to its consideration as a potential anticancer agent. Although previous studies have demonstrated the effect of FBZ on cancer cells, there is a paucity of studies on the effect of FBZ on lymphoma cells and normal immune cells. Herein, we investigated the effects of FBZ on a mouse lymphoma cell line, EL-4 cells, and spleen cells, using vincristine as a positive control. The cellular metabolic activity of EL-4 cells was decreased by FBZ, but that of the spleen cells was not decreased. Moreover, FBZ reduced the mitochondrial membrane potential and induced reactive oxygen species production in EL-4 cells, but not in spleen cells. FBZ induced G2/M phase arrest and increased the sub G0/G1 phase ratio, indicating apoptosis. Furthermore, compared to the control cells, the reactivity of spleen cells pretreated with FBZ to lipopolysaccharide was maintained. In summary, FBZ is cytotoxic to EL-4 cells, but not to spleen cells. This study provides experimental evidence that FBZ exerts an anticancer effect, and less cytotoxic effects and functional damage to normal spleen cells.
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芬苯达唑对小鼠淋巴瘤EL-4细胞和脾细胞的差异细胞毒作用
芬苯咪唑(FBZ)是一种广泛用于治疗寄生虫感染的苯并咪唑类驱虫剂。FBZ的抗癌作用最近得到了强调,因此被认为是一种潜在的抗癌剂。尽管先前的研究已经证明了FBZ对癌症细胞的影响,但关于FBZ对淋巴瘤细胞和正常免疫细胞的影响的研究很少。在此,我们使用长春新碱作为阳性对照,研究了FBZ对小鼠淋巴瘤细胞系、EL-4细胞和脾细胞的影响。FBZ降低了EL-4细胞的细胞代谢活性,但脾脏细胞的代谢活性没有降低。此外,FBZ降低了EL-4细胞的线粒体膜电位并诱导活性氧的产生,但在脾细胞中没有。FBZ诱导G2/M期阻滞,并增加亚G0/G1期比率,表明细胞凋亡。此外,与对照细胞相比,用FBZ预处理的脾细胞对脂多糖的反应性得以维持。总之,FBZ对EL-4细胞具有细胞毒性,但对脾细胞没有细胞毒性。本研究提供了实验证据,证明FBZ具有抗癌作用,对正常脾细胞的细胞毒性作用和功能损伤较小。
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来源期刊
Korean Journal of Veterinary Research
Korean Journal of Veterinary Research Veterinary-Veterinary (all)
CiteScore
0.50
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0.00%
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