Similarity and Phylogenetic Analysis of Herbicide-Resistant Goosegrass (Eleusine indica) Biotypes

Q3 Agricultural and Biological Sciences Caraka Tani Journal of Sustainable Agriculture Pub Date : 2019-08-08 DOI:10.20961/carakatani.v34i2.29156

Koko Tampubolon, Fransisca Natalia Sihombing, Elseria Siburian, Y. Sulastri, Zavandri Purba, Sony Tri Septian Samosir, Syahibal Karim

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Abstract

Molecular approach as a herbicide-resistant agent detector is an initial solution before controlling of Eleusine indica weed. This review article is aimed to obtain the basic data of similarity and phylogenetic values among herbicide-resistant E. indica biotypes. This research used a descriptive analytical method. The analysis involved 14 nucleotide sequences of herbicide-resistant E.indica biotypes obtained from the National Center for Biotechnology Information. The nucleotide alignment of herbicide-resistant E. indica biotypes was conducted with ClustaLW using the Molecular Evolutionary Genetics Analysis (MEGA) v. 5.05 software based on a method of neighbor-joining tree construct/test. The results showed that two nucleotides of 7,921 herbicide-resistant E. indica biotypes were homologous (sequence 1,231 and sequence 1,408). The similarity values among herbicide-resistant E. indica biotypes ranged from 0.00 to 1.19. The information of phylogenetic pattern is needed in the selection of the herbicides mode of action rotation in order to control herbicide-resistant E. indica biotypes.

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抗除草剂鹅草(Eleusine indica)生物型相似性及系统发育分析

分子方法作为除草剂抗性剂的检测器是在控制Eleusine indica杂草之前的初步解决方案。本文旨在获得抗除草剂籼稻生物型的相似性和系统发育价值的基本数据。本研究采用描述性分析方法。该分析涉及从国家生物技术信息中心获得的抗除草剂E.indica生物型的14个核苷酸序列。使用基于邻居连接树构建/测试方法的分子进化遗传学分析（MEGA）v.5.05软件，用ClustaLW对耐除草剂的籼稻生物型进行核苷酸比对。结果表明，7921个抗除草剂籼稻生物型的两个核苷酸具有同源性（序列1231和序列1408）。抗除草剂籼稻生物型间的相似性在0.00～1.19之间。在选择除草剂的作用模式轮换时，需要系统发育模式的信息来控制抗除草剂的籼稻生物型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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