Anti-HIV Mechanism of Sulfated Poly and Oligosaccharides

Abstract

: This review focuses on the previous and recent results as well as related literatures regarding the anti-HIV mechanism of sulfated alkyl poly- and oligosaccharides. and positively charged amino acids in HIV gp120. The mechanism was assumed to be similar to the electrostatic interaction between a natural blood anticoagulant sulfated polysaccharide heparin and a protease inhibitor antithrombin III. In addition, the anti-HIV mechanism of curdlan sulfate was quantitatively investigated using surface plasmon resonance (SPR) and dynamic light scattering (DLS) measured with oligopeptides from three regions in HIV gp120, V3 loop, C-terminus, and CD4 binding domain. These studies revealed the interaction between oligopeptides of the V3 loop and C-terminus bearing positively charged amino acid accumulated regions in each sequence. These results indicated that the anti-HIV activity of sulfated polysaccharides involves electrostatic interactions. It was reported that a long-chain alkyl group in sulfated alkyl oligosaccharides plays a key role in the enhancement of anti-HIV activity. The interaction between sulfated alkyl poly- and oligosaccharides and liposomes as a model of HIV was also discussed by SPR and DLS measurements, suggesting that the long-chain alkyl group penetrated into the lipid bilayer of HIV, and then sulfated poly- and oligosaccharide portions electrostatically interacted with HIV gp120 to produce potent anti-HIV activity. 3- O octadecyl dextran. These results were suggestive for the potent anti-HIV and cytotoxicity of sulfated alkyl poly-and