Phytochemical and antibacterial properties of root extracts from Portulaca oleracea Linn. (Purslane) utilised in the management of diseases in Nigeria

Q4 Economics, Econometrics and Finance Journal of Medicinal Plants for Economic Development Pub Date : 2021-01-26 DOI:10.4102/JOMPED.V5I1.103
E. Ojah, Emmanuel O. Oladele, Philip Chukwuemeka
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引用次数: 6

Abstract

Background: Bacteria as etiological agents have been reported to cause many diseases and have increased the rate of mortality globally. Their resistance to conventional medicine has made medicinal plants a credible alternative in the management of diseases caused by bacterial infection. In the recent times many research efforts have been directed towards the exploration of phytoconstituents with antibacterial potentials. Medicinal plants are widely used as antibacterial agents because of their high therapeutic performance, low toxicity, and affordability. Aim: This work was designed to identify secondary metabolites present in root extracts of ethno-medicinally utilised Portulaca oleracea L. and evaluate their antibacterial activities. Setting: The roots of P. oleracea L. were obtained from the Forest Research Institute of Nigeria (FRIN), Ibadan, Nigeria and authenticated in the Forest Research Herbarium, where voucher samples were deposited with specimen voucher number FIH-112030. Methods: Phytochemical screening was carried out using standard qualitative tests and the antibacterial activity of extracts was evaluated using agar well diffusion method whilst the minimum inhibitory concentration (MIC) was evaluated by micro-dilution method. The screening was assessed against Bacillus subtilis, Candida albicans, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Micrococcus luteus, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysenteriae, Staphylococcus aureus and Streptococcus agalactiae , which are responsible for the transmission of common diseases in Nigeria. Statistical analysis was performed by one-way analysis of variance (ANOVA) with GraphPad Prism 8.0 and results were expressed as mean ± s.d. Duncan’s New Multiple range test were applied at 0.05 level of significance ( p < 0.05). Results: Phytochemical screening of P. oleracea L. showed the presence of carbohydrates, steroids, triterpenes, cardiac glycosides, and saponins. All extracts showed a high level of minimum inhibition concentration against the pathogens except K. pneumoniae, M. luteus and P. aeruginosa. Generally the antibacterial activity of extracts increased with decrease in polarity as compared with ciprofloxacin. The mean (± s.d.) values were significantly different by Duncan’s multiple range tests with p < 0.05. Conclusion: Portulaca oleracea L. has been identified for the first time as a good antibacterial agent, which corroborates the ethno-medicinal uses of the plant.
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马齿苋根提取物的植物化学和抗菌性能。(Purslane)在尼日利亚用于疾病管理
背景:据报道,细菌作为病原体会导致许多疾病,并在全球范围内增加死亡率。它们对传统药物的耐药性使药用植物成为治疗细菌感染引起的疾病的可靠替代品。近年来,许多研究工作都致力于探索具有抗菌潜力的植物成分。药用植物因其高治疗性能、低毒性和可负担性而被广泛用作抗菌剂。目的:本研究旨在鉴定民族药用马齿苋根提取物中的次生代谢产物,并评价其抗菌活性。环境:P.oleracea L.的根从尼日利亚伊巴丹的尼日利亚森林研究所(FRIN)获得,并在森林研究植物标本馆进行鉴定,凭证样品存放在凭证号为FIH-112030的标本库中。方法:采用标准定性试验进行植物化学筛选,采用琼脂扩散法评价提取物的抗菌活性,采用微量稀释法评价最低抑菌浓度。对枯草芽孢杆菌、白色念珠菌、阴沟肠杆菌、大肠杆菌、肺炎克雷伯菌、黄体微球菌、铜绿假单胞菌、伤寒沙门氏菌、痢疾志贺菌、金黄色葡萄球菌和无乳链球菌进行了筛查,这些疾病是尼日利亚常见疾病的传播原因。采用GraphPad Prism 8.0单因素方差分析(ANOVA)进行统计分析,结果以平均值±标准差表示。Duncan’s New Multiple range检验的显著性水平为0.05(p<0.05)。除肺炎克雷伯菌、黄曲霉和铜绿假单胞菌外,所有提取物对病原体均显示出高水平的最低抑制浓度。与环丙沙星相比,提取物的抗菌活性通常随着极性的降低而增加。Duncan多重范围检验的平均值(±s.d.)有显著差异,p<0.05。结论:马齿苋首次被鉴定为一种良好的抗菌剂,证实了该植物的民族药用价值。
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来源期刊
Journal of Medicinal Plants for Economic Development
Journal of Medicinal Plants for Economic Development Economics, Econometrics and Finance-Economics and Econometrics
CiteScore
0.90
自引率
0.00%
发文量
14
审稿时长
32 weeks
期刊最新文献
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