Transcriptional Analysis of Mice Melanoma B16-F10 Cells in Response to Directed Current Electric Fields

IF 1.8 3区生物学 Q3 BIOLOGY Bioelectromagnetics Pub Date : 2022-05-31 DOI:10.1002/bem.22412

Jinlei Yun, Xiaoli Jin, Qin Sun, Linfeng Xu, Jing Gao, Xiaoyan Wang, Sanjun Zhao

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Abstract

The endogenous electric field (EF) is widely observed among tissues. It is supposed to be an important environmental factor in tumor metastasis. To explore the role of endogenous EFs in tumor metastasis, the migration of mouse melanoma B16-F10 cells in directed current EFs (dcEFs) was investigated. The transcriptome of melanoma B16-F10 cells in response to EF stimulation was analyzed using RNA sequencing. The results demonstrated that the mouse melanoma B16-F10 cells migrated toward the cathode in applied dcEFs. Directional migration occurred in a voltage-dependent manner. Approximately 3000 upregulated and 2613 downregulated genes were identified under dcEF. Some genes correlated with cell migration, such as Serpine1, Ctgf, Fosb, and Fos, were upregulated. The signaling pathways involved in cell motility were significantly altered. Some genes, highly related to tumorigenesis, invasion, and metastasis, are upregulated in response to EF stimulation. Endogenous EFs may play a role in tumorigenesis and metastasis in vivo. © 2022 Bioelectromagnetics Society.

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定向电流电场作用下小鼠黑色素瘤B16-F10细胞的转录分析

内源电场(EF)在组织中广泛存在。它被认为是肿瘤转移的重要环境因素。为了探讨内源性电场在肿瘤转移中的作用，我们研究了小鼠黑色素瘤B16-F10细胞在定向电流电场(dcEFs)中的迁移。利用RNA测序分析黑色素瘤B16-F10细胞在EF刺激下的转录组。结果表明，小鼠黑色素瘤B16-F10细胞在应用dcEFs中向阴极迁移。定向迁移以电压依赖的方式发生。在dcEF下鉴定出大约3000个上调基因和2613个下调基因。一些与细胞迁移相关的基因，如Serpine1、Ctgf、Fosb和Fos表达上调。参与细胞运动的信号通路明显改变。一些与肿瘤发生、侵袭和转移高度相关的基因在EF刺激下被上调。内源性EFs可能在体内肿瘤发生和转移中起作用。©2022生物电磁学学会。

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来源期刊

Bioelectromagnetics 生物-生物物理

CiteScore

4.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Bioelectromagnetics is published by Wiley-Liss, Inc., for the Bioelectromagnetics Society and is the official journal of the Bioelectromagnetics Society and the European Bioelectromagnetics Association. It is a peer-reviewed, internationally circulated scientific journal that specializes in reporting original data on biological effects and applications of electromagnetic fields that range in frequency from zero hertz (static fields) to the terahertz undulations and visible light. Both experimental and clinical data are of interest to the journal''s readers as are theoretical papers or reviews that offer novel insights into or criticism of contemporary concepts and theories of field-body interactions. The Bioelectromagnetics Society, which sponsors the journal, also welcomes experimental or clinical papers on the domains of sonic and ultrasonic radiation.