Effect of Dahuang Zhechong pills on long non-coding RNA growth arrest specific 5 in rat models of hepatic fibrosis

IF 2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional Chinese Medicine Pub Date : 2018-04-01 DOI:10.1016/j.jtcm.2018.04.007

Gong Zhenghua , Deng Chaowen , Xiang Tianxin , Tao Lili , Xiao Hongbo , Peng Yanzhong , Zheng Jie , Hu Guoxin

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引用次数: 8

Abstract

OBJECTIVE

To investigate the involvement of growth arrest specific 5 (GAS5), a long non-coding RNA, in the anti-hepatic fibrosis process induced by Dahuang Zhechong pill (DHZCP) in rats.

METHODS

Thirty adult rats were divided into three groups, including a control group, a CCl₄-induced fibrosis group and a DHZCP-treated fibrosis group. Hematoxylin-eosin and Masson staining were used for histopathological study. Serum enzymes, cytokines and cell proliferation were assayed using commercially available kits. A GAS5 lenti-virus vector was constructed to further investigate the role of GAS5 in the anti-hepatic fibrosis effect of DHZCP in rats.

RESULTS

Our results revealed that the proliferation of hepatic stellate cells cultured in serum derived from rats treated with DHZCP was significantly decreased, compared with cells treated with serum from the untreated rats. DHZCP alleviated the CCl₄-induced hepatic fibrosis. Additionally, DHZCP can restore the expression of GAS5, which was significantly decreased in the CCl₄-induced group, and markedly suppress the expression of p-p38 and p-Erk induced by CCl_4, but not p-Jnk. Cell proliferation was significantly arrested when cells overexpressed GAS5. Thus, DHZCP can inhibit the expression of p-p38 and p-Erk, while GAS5 can only inhibit the expression of p-Erk.

CONCLUSIONS

DHZCP can alleviate hepatic fibrosis by increasing the expression of GAS5 to suppress p-Erk and regulating other factors to inhibit p-p38.

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大黄浙冲丸对大鼠肝纤维化模型长链非编码RNA生长阻滞特异性5的影响

目的探讨长链非编码RNA生长抑制特异性5 (GAS5)在大黄止血丸(DHZCP)诱导大鼠肝纤维化过程中的作用。方法30只成年大鼠分为3组，分别为对照组、ccl4诱导纤维化组和dhzcp治疗纤维化组。采用苏木精-伊红染色和马松染色进行组织病理学研究。使用市售试剂盒检测血清酶、细胞因子和细胞增殖。构建GAS5慢病毒载体，进一步研究GAS5在DHZCP抗大鼠肝纤维化中的作用。结果经DHZCP处理的大鼠血清中培养的肝星状细胞增殖能力明显低于未处理的大鼠血清。DHZCP可减轻ccl4诱导的肝纤维化。此外，DHZCP能恢复CCl4诱导组显著降低的GAS5的表达，并能显著抑制CCl4诱导的p-p38和p-Erk的表达，但对p-Jnk无抑制作用。当细胞过表达GAS5时，细胞增殖明显受阻。因此，DHZCP可以抑制p-p38和p-Erk的表达，而GAS5只能抑制p-Erk的表达。结论sdhzcp通过增加GAS5的表达抑制p-Erk，调节其他因子抑制p-p38来减轻肝纤维化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

2.40

自引率

3.80%

发文量

32269

审稿时长

2 months

期刊介绍： Journal of Traditional Chinese Medicine（JTCM） is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.